A Comparative Study of Aspirin Loaded Bovine Serum Albumin Nanoparticles Prepared by Desolvation Technique Using Various Desolvating Agents

Abstract

Aspirin is mainly used in the treatment of rheumatoid arthtitis and ankylosing spondylitis (AS). The dose of aspirin required for the treatment is 3 g / day in divided doses. In order to avoid chances of missing the dose of drug, it is better to formulate sustained release dosage forms. In the present study, aspirin loaded bovine serum albumin (BSA) nanoparticles were prepared by desolvation technique using various desolvating agents such as acetone, ethanol and sodium sulphate. A comparative study was made among the three desolvating agents (acetone, ethanol and sodium sulphate) and two methods (continuous addition method and intermittent addition method) to decide the best desolvating agent and the better method for preparation of aspirin nanoparticles by desolvation method. Continuous and intermittent addition methods were followed for the addition of desolvating agent to the aqueous solution of bovine serum albumin. Bovine serum albumin nanoparticles prepared by intermittent addition of ethanol was showing better results with the mean particle diameter of 209 nm, entrapment efficiency of 50% and loading capacity of 23%. The drug release was slow, extending over a period of 24 h. The curve fitting data revealed that the release followed the first-order kinetics. Higuchis and Peppas plots stated that Fickian diffusion controlled the pattern in all formulations. From the results it can be concluded that the method of intermittent addition was the better method and ethanol was the best desolvating agent for the preparation of aspirin nanoparticles by desolvation technique.

Factors Affecting the Synthesis of Bovine Serum Albumin Nanoparticles Using the Desolvation Method

“…Sailaja 90 also showed that ethanol is the best desolvation agent because it produces nanoparticles with smaller sizes and better entrapment efficiency compared to acetone and sodium sulphate. This study identified two important characteristics of the desolvation agents contributing to the production of large nanoparticles.…”

“…In this context, ethanol can act as a hydrogen donor and acceptor, producing nanoparticles better than acetone. 90 …”

Preparation and Characterization of Nanoparticulate Drug Delivery System for Naproxen Sodium Using Various Desolvating Agents

“…Naproxen sodium and BSA prepared nanoparticles were mixed separately with finely powdered potassium bromide IR [9]. Grind the mixture thoroughly, spread it uniformly in a suitable die and compressed under vacuum pressure of about 8000 metric tons of pressure in a hydraulic press, and the pellets were scanned over a wave number range of 4000 to 400 cm −1 in a FTIR instrument [9]. Mount the resultant disc in a suitable holder in the spectrophotometer [20].…”

“…The percentage entrapment efficiency was calculated with the following formula: Entrapment efficiency [9] = (Amount of drug encapsulated in the formulation/Total amount of drug in the formulation) × 100.…”

“…Loading capacity was calculated from the following formula: Loading capacity [9] = (Total amount of the drug I amount of free drug concentration / Nanoparticles' weight) × 100.…”