Roles of alcohol desolvating agents on the size control of bovine serum albumin nanoparticles in drug delivery system

Sun, Si; Xiao, Qian‐Ru; Wei, Xiawei; Jiang, Yong

doi:10.1016/j.jddst.2018.07.018

Cited by 20 publications

(13 citation statements)

References 31 publications

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“…Pei et al, assumed that differences in alcohols’ viscosity can influence the desolvation of gelatin [ 43 ]. As a rule, smaller nanoparticles were obtained using ethanol, which is in line with the previous reports [ 51 ]. This difference, though, was more distinct at the gelatin concentration of 18 mg/mL.…”

Section: Resultssupporting

confidence: 90%

“…It has been reported that the desolvation of α-lactalbumin by isopropyl alcohol provided the largest nanoparticles [ 50 ]. Moreover, a lower volume of isopropyl alcohol is required to completely desolvate bovine serum albumin in comparison with ethanol [ 51 ]. A more complex relationship between properties of non-solvent and its influence on the size of nanoparticles, not limited to the difference of dielectric constants, was demonstrated by Mohammad-Beigi et al [ 52 ].…”

Section: Resultsmentioning

confidence: 99%

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Modified Desolvation Method Enables Simple One-Step Synthesis of Gelatin Nanoparticles from Different Gelatin Types with Any Bloom Values

et al. 2021

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Gelatin nanoparticles found numerous applications in drug delivery, bioimaging, immunotherapy, and vaccine development as well as in biotechnology and food science. Synthesis of gelatin nanoparticles is usually made by a two-step desolvation method, which, despite providing stable and homogeneous nanoparticles, has many limitations, namely complex procedure, low yields, and poor reproducibility of the first desolvation step. Herein, we present a modified one-step desolvation method, which enables the quick, simple, and reproducible synthesis of gelatin nanoparticles. Using the proposed method one can prepare gelatin nanoparticles from any type of gelatin with any bloom number, even with the lowest ones, which remains unattainable for the traditional two-step technique. The method relies on quick one-time addition of poor solvent (preferably isopropyl alcohol) to gelatin solution in the absence of stirring. We applied the modified desolvation method to synthesize nanoparticles from porcine, bovine, and fish gelatin with bloom values from 62 to 225 on the hundreds-of-milligram scale. Synthesized nanoparticles had average diameters between 130 and 190 nm and narrow size distribution. Yields of synthesis were 62–82% and can be further increased. Gelatin nanoparticles have good colloidal stability and withstand autoclaving. Moreover, they were non-toxic to human immune cells.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Modified Desolvation Method Enables Simple One-Step Synthesis of Gelatin Nanoparticles from Different Gelatin Types with Any Bloom Values

et al. 2021

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show abstract

“…Pei et al assumed that differences in alcohols' viscosity can influence the desolvation of gelatin (Pei, 2021). As a rule, smaller nanoparticles were obtained using ethanol, which is in line with the previous reports (Sun, 2018). This difference, though, was more distinct at the gelatin concentration of 18 mg/ml.…”

Section: The Second Part Of Optimization Experimentssupporting

confidence: 75%

“…It has been reported that the desolvation of α-lactalbumin by isopropyl alcohol provided the largest nanoparticles (Arroyo-Maya, 2012). moreover, a lower volume of isopropyl alcohol is required to completely desolvate BSA in comparison with ethanol (Sun, 2018). A more complex relationship between properties of non-solvent and its influence on the size of nanoparticles, not limited to the difference of dielectric constants, was demonstrated by Mohammad-Beigi et al (Mohammad-Beigi, 2016).…”

Section: The Second Part Of Optimization Experimentsmentioning

confidence: 99%

Modified desolvation method enables simple one-step synthesis of gelatin nanoparticles from different gelatin types with any bloom values

Khramtsov

Burdina

Lazarev

et al. 2021

Preprint

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Gelatin nanoparticles found numerous applications in drug delivery, bioimaging, immunotherapy, and vaccine development as well as in biotechnology and food science. Synthesis of gelatin nanoparticles is usually made by a two-step desolvation method, which, despite providing stable and homogeneous nanoparticles, has many limitations, namely complex procedure, low yields, and poor reproducibility of the first desolvation step. Herein, we present a modified one-step desolvation method, which enables the quick, simple, and reproducible synthesis of gelatin nanoparticles. Using the proposed method one can prepare gelatin nanoparticles from any type of gelatin with any bloom number, even with the lowest ones, which remains unattainable for the traditional two-step technique. The method relies on quick one-time addition of poor solvent (preferably isopropyl alcohol) to gelatin solution in the absence of stirring. We applied the modified desolvation method to synthesize nanoparticles from porcine, bovine, and fish with bloom values from 62 to 225 on the hundreds-of-milligram scale. Synthesized nanoparticles had average diameters between 130 and 190 nm and narrow size distribution. Yields of synthesis were 62-82% and can be further increased. Gelatin nanoparticles have good colloidal stability and withstand autoclaving. Moreover, they were non-toxic to human immune cells.

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“…The increase in the rate of dissolution of the drug was demonstrated [22]. The effectiveness of BSA as a drug carrier was also shown earlier [23,24]. The use of BSA allows reducing cytotoxicity of the drug by a factor of 9 [25].…”

Section: Introductionmentioning

confidence: 54%

Formation of microspherical particles of albumin with model drug using spray drying process

2019

Biointerface Res Appl Chem

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Spray drying method enables to produce microspherical particles with narrow size distribution. Production of such microparticles is relevant for the development of the advanced systems for inhalation delivery of drugs. The aerosol particles diameter must be between 1 and 3 μm. Composite microparticles based on bovine serum albumin and model drug phenacetin were produced in the present work using the spray-drying method. The absence of a crystalline phase of the drug in composite microparticles was verified using several physicochemical methods. The average radius of the produced microparticles was found to be 1 μm based on the results from scanning electron microscopy. The rate of the dissolution of the drug included in microparticles is faster than that of crystalline phenacetin. The obtained results can be used for the development of the method for preparation of composite microparticles based on protein molecules using the spray-drying technique.

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Roles of alcohol desolvating agents on the size control of bovine serum albumin nanoparticles in drug delivery system

Cited by 20 publications

References 31 publications

Modified Desolvation Method Enables Simple One-Step Synthesis of Gelatin Nanoparticles from Different Gelatin Types with Any Bloom Values

Modified Desolvation Method Enables Simple One-Step Synthesis of Gelatin Nanoparticles from Different Gelatin Types with Any Bloom Values

Modified desolvation method enables simple one-step synthesis of gelatin nanoparticles from different gelatin types with any bloom values

Formation of microspherical particles of albumin with model drug using spray drying process

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