A calcium-dependent lipid binding protein, OsANN10, is a negative regulator of osmotic stress tolerance in rice

Gao, Shuxin; Song, Tao; Han, Jianxun; He, Mengli; Zhang, Qian; Zhu, Ying; Zhang, Zhu

doi:10.1016/j.plantsci.2020.110420

Cited by 20 publications

(26 citation statements)

References 57 publications

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“…Based on bioinformatics, there are ten putative annexin genes in the rice genome, and we previously reported that OsANN1 , OsANN3 and OsANN10 are involved in the response to heat, drought and osmotic stress [ 36 – 38 ]. To obtain a more comprehensive understanding of the function of rice annexin, a putative annexin family gene (LOC_Os05g31750) was cloned from Oryza sativa L. spp.…”

Section: Resultsmentioning

confidence: 99%

“…Excessive accumulation of ROS can cause toxic effects on proteins, lipids and nucleic acids, and the antioxidant defense system consisting of enzymatic and nonenzymatic antioxidants is essential for scavenging excess ROS [ 17 , 18 ]. Evidence has shown that plant annexins have peroxidase activity and respond to abiotic stress by regulating the production of ROS [ 30 , 37 , 38 , 45 ]. OsANN1 -overexpressing plants eliminated excess ROS by increasing peroxidase activity, thereby improving the tolerance of rice to heat stress [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…Rice annexin OsANN3 regulates the drought stress response in an ABA-dependent pathway [ 36 ]. Both OsANN1 and OsANN10 confer resistance to abiotic stress in rice by modulating ROS and/or lipid peroxidation levels [ 37 , 38 ]. To date, AtANN1 is the most widely studied plant annexin, and it functions in Arabidopsis in response to a variety of abiotic stresses (such as osmotic stress, drought, salt, ABA, cold, heat and hydrogen peroxide) [ 39 – 44 ].…”

Section: Introductionmentioning

confidence: 99%

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OsANN4 modulates ROS production and mediates Ca2+ influx in response to ABA

Zhang

Song

et al. 2021

BMC Plant Biol

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Background Plant annexins are calcium- and lipid-binding proteins that have multiple functions, and a significant amount of research on plant annexins has been reported in recent years. However, the functions of annexins in diverse biological processes in rice are largely unclear. Results Herein, we report that OsANN4, a calcium-binding rice annexin protein, was induced by abscisic acid (ABA). Under ABA treatment, the plants in which OsANN4 was knocked down by RNA interference showed some visible phenotypic changes compared to the wild type, such as a lower rooting rate and shorter shoot and root lengths. Moreover, the superoxide dismutase (SOD) and catalase (CAT) activities of the RNAi lines were significantly lower and further resulted in higher accumulation of O2.- and H2O2 than those of the wild-type. A Non-invasive Micro-test Technology (NMT) assay showed that ABA-induced net Ca2+ influx was inhibited in OsANN4 knockdown plants. Interestingly, the phenotypic differences caused by ABA were eliminated in the presence of LaCl3 (Ca2+ channel inhibitor). Apart from this, we demonstrated that OsCDPK24 interacted with and phosphorylated OsANN4. When the phosphorylated serine residue of OsANN4 was substituted by alanine, the interaction between OsANN4 and OsCDPK24 was still observed, however, both the conformation of OsANN4 and its binding activity with Ca2+ might be changed. Conclusions OsANN4 plays a crucial role in the ABA response, partially by modulating ROS production, mediating Ca2+ influx or interacting with OsCDPK24.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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OsANN4 modulates ROS production and mediates Ca2+ influx in response to ABA

Zhang

Song

et al. 2021

BMC Plant Biol

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“…Wheat annexin, TdAnn6 , participates in resistance to salt and osmotic stress by regulating antioxidant mechanisms [ 28 ]. OsAnn10 is a negative regulator of resistance to osmotic stress in rice ( Oryza sativa ) [ 47 ], while OsAnn1 enhances heat stress tolerance [ 48 ]. The cotton ( Gossypium hirsutum ) annexin GhANN8b participates in the regulating of Ca 2+ and Na + fluxes in plant salt tolerance.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of Cassava MeAnn2 Enhances the Salt and IAA Tolerance of Transgenic Arabidopsis

Lin

Zhou

et al. 2021

Plants

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Annexins are a superfamily of soluble calcium-dependent phospholipid-binding proteins that have considerable regulatory effects in plants, especially in response to adversity and stress. The Arabidopsis thaliana AtAnn1 gene has been reported to play a significant role in various abiotic stress responses. In our study, the cDNA of an annexin gene highly similar to AtAnn1 was isolated from the cassava genome and named MeAnn2. It contains domains specific to annexins, including four annexin repeat sequences (I–IV), a Ca2+-binding sequence, Ca2+-independent membrane-binding-related tryptophan residues, and a salt bridge-related domain. MeAnn2 is localized in the cell membrane and cytoplasm, and it was found to be preferentially expressed in the storage roots of cassava. The overexpression of MeAnn2 reduced the sensitivity of transgenic Arabidopsis to various Ca2+, NaCl, and indole-3-acetic acid (IAA) concentrations. The expression of the stress resistance-related gene AtRD29B and auxin signaling pathway-related genes AtIAA4 and AtLBD18 in transgenic Arabidopsis was significantly increased under salt stress, while the Malondialdehyde (MDA) content was significantly lower than that of the control. These results indicate that the MeAnn2 gene may increase the salt tolerance of transgenic Arabidopsis via the IAA signaling pathway.

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“…These results indicate potential differences between periods and changes in P content in the phosphorylation modification of Pi homeostasis. As CaLB, annexins (such as AtANN1, OsANN1, OsANN3, and OsANN10) may be involved in Ca 2+ channel regulation, peroxidase, and ATPase/GTPase activities in response to abiotic stress (Richards et al, 2013;Qiao et al, 2015;Li et al, 2019;Gao et al, 2020). In this study, annexin 4 (HORVU1Hr1G057170.11) had significantly increased phosphorylation levels under the Pi resupply condition.…”

Section: Phosphoproteins Involved In Signal Transductionmentioning

confidence: 58%

Global Profiling of Phosphorylation Reveals the Barley Roots Response to Phosphorus Starvation and Resupply

Wang

et al. 2021

Front. Plant Sci.

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Phosphorus (P) deficiency is a major threat to the crop production, and for understanding the response mechanism of plant roots, P stress may facilitate the development of crops with increased tolerance. Phosphorylation plays a critical role in the regulation of proteins for plant responses to biotic and abiotic stress; however, its functions in P starvation/resupply are largely unknown for barley (Hordeum vulgare) growth. Here, we performed a global review of phosphorylation in barley roots treated by P starvation/resupply. We identified 7,710 phosphorylation sites on 3,373 proteins, of which 76 types of conserved motifs were extracted from 10,428 phosphorylated peptides. Most phosphorylated proteins were located in the nucleus (36%) and chloroplast (32%). Compared with the control, 186 and 131 phosphorylated proteins under P starvation condition and 156 and 111 phosphorylated proteins under P resupply condition showed significant differences at 6 and 48 h, respectively. These proteins mainly participated in carbohydrate metabolism, phytohormones, signal transduction, cell wall stress, and oxidases stress. Moreover, the pathways of the ribosome, RNA binding, protein transport, and metal binding were significantly enriched under P starvation, and only two pathways of ribosome and RNA binding were greatly enriched under Pi resupply according to the protein–protein interaction analysis. The results suggested that the phosphorylation proteins might play important roles in the metabolic processes of barley roots in response to Pi deficiency/resupply. The data not only provide unique access to phosphorylation reprogramming of plant roots under deficiency/resupply but also demonstrate the close cooperation between these phosphorylation proteins and key metabolic functions.

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A calcium-dependent lipid binding protein, OsANN10, is a negative regulator of osmotic stress tolerance in rice

Cited by 20 publications

References 57 publications

OsANN4 modulates ROS production and mediates Ca2+ influx in response to ABA

OsANN4 modulates ROS production and mediates Ca2+ influx in response to ABA

Overexpression of Cassava MeAnn2 Enhances the Salt and IAA Tolerance of Transgenic Arabidopsis

Global Profiling of Phosphorylation Reveals the Barley Roots Response to Phosphorus Starvation and Resupply

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