Overexpression of Cassava MeAnn2 Enhances the Salt and IAA Tolerance of Transgenic Arabidopsis

Lin, Xue‐Jun; Li, Ruimei; Zhou, Yangjiao; Tang, Fenlian; Wang, Yajie; Lü, Xiaohua; Wang, Shijia; Yao, Yuan; Liu, Jiao; Hu, Xinwen; Guo, Jiao

doi:10.3390/plants10050941

Cited by 7 publications

(5 citation statements)

References 54 publications

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“…However, annexin-regulated transport of plant hormone has rarely beenreported. An annexin in cassava, MeANN2, which is similar to AtANN1, is involved in stress tolerance via regulating auxin signaling(Lin et al 2021). Our results provide direct evidence for the involvement of AtANN3 in regulating auxin transport in response to an extracellular signal.Auxin e ux transporters, especially PINs, play essential roles in polar auxin transport and asymmetric distribution-induced physiological responses.…”

supporting

confidence: 52%

ATANN3 is involved in extracellular ATP-regulated auxin transport and distribution in Arabidopsis thaliana seedlings

Shang

Han

et al. 2022

Preprint

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Extracellular ATP (eATP) exists in the apoplast and plays multiple roles in growth, development, and stress responses. eATP has been revealed to suppresses growth rate and alters growth orientation of root and hypocotyl of Arabidopsis thalianaseedlings by affecting auxin transport in these organs. However, the mechanism of eATP-stimulated auxin distribution remains unclear. Annexins are involved in multiple aspects of plant cellular metabolism, while the role of annexins in response to apoplast signal remains unclear. Here, by using loss-of-function mutants, we investigated the role of several annexins in eATP-regulated root and hypocotyl growth. Since mutants of AtANN3 did not respond to eATP sensitively, the role of AtANN3 in eATP regulated auxin transport was intensively investigated. Firstly, the inhibitory effect of eATP on root or hypocotyl elongation was weakened or impaired in AtANN3 null mutants (atann3-1 and atann3-2). Meanwhile, single-, double- or triple-null mutant of AtANN1, AtANN2or AtANN4 responded to eATP in same manner and degree with Col-0. The distribution of DR5-GUS and DR5-GFP indicated that eATP-induced asymmetric distribution of auxin in root tip or hypocotyl cells, which appeared in wild type controls, were lacking in atann3-1seedlings. Further, eATP-induced asymmetric distribution of PIN2-GFP in root tip cells or PIN3-GFP in hypocotyl cells were reduced in atann3-1 seedlings. Based on these results, we suggest that AtANN3 may be involved in eATP-regulated seedling growth through regulating auxin transport in vegetative organs.

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supporting

confidence: 52%

ATANN3 is involved in extracellular ATP-regulated auxin transport and distribution in Arabidopsis thaliana seedlings

Shang

Han

et al. 2022

Preprint

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“…Some annexins have been revealed to be involved in the regulation of auxin transport and distribution. For instance, AtANN1 and AtANN2 are involved in unilateral blue-light-induced asymmetric distribution of auxin and PINs in etiolated hypocotyls [ 78 ] and MeANN2 in cassava, which is similar to AtANN1, is involved in stress tolerance via regulating auxin signaling [ 79 ]. Our results provide direct evidence for the involvement of AtANN3 in regulating auxin transport in response to an extracellular signal.…”

Section: Discussionmentioning

confidence: 99%

ATANN3 Is Involved in Extracellular ATP-Regulated Auxin Distribution in Arabidopsis thaliana Seedlings

Han

Xia

et al. 2023

Plants

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Extracellular ATP (eATP) plays multiple roles in plant growth and development, and stress responses. It has been revealed that eATP suppresses growth and alters the growth orientation of the root and hypocotyl of Arabidopsis thaliana by affecting auxin transport and localization in these organs. However, the mechanism of the eATP-stimulated auxin distribution remains elusive. Annexins are involved in multiple aspects of plant cellular metabolism, while their role in response to apoplastic signals remains unclear. Here, by using the loss-of-function mutations, we investigated the role of AtANN3 in the eATP-regulated root and hypocotyl growth. Firstly, the inhibitory effects of eATP on root and hypocotyl elongation were weakened or impaired in the AtANN3 null mutants (atann3–1 and atann3–2). Meanwhile, the distribution of DR5-GUS and DR5-GFP indicated that the eATP-induced asymmetric distribution of auxin in the root tips or hypocotyl cells occurred in wild-type control plants, while in atann3–1 mutant seedlings, it was not observed. Further, the eATP-induced asymmetric distribution of PIN2-GFP in root-tip cells or that of PIN3-GFP in hypocotyl cells was reduced in atann3–1 seedlings. Finally, the eATP-induced asymmetric distribution of cytoplasmic vesicles in root-tip cells was impaired in atann3–1 seedlings. Based on these results, we suggest that AtANN3 may be involved in eATP-regulated seedling growth by regulating the distribution of auxin and auxin transporters in vegetative organs.

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“…Then, a dual-wavelength fluorescent protein excitation light source (Luyor-3415RG, Luyor Corporation, Shanghai, China) was used to irradiate the germinated seedlings. Positive transgenic Arabidopsis seedlings were selected, as they showed green fluorescence under light, while the wild type and transgenic lines with too-low gene expression showed a red color [59].…”

Section: Fluorescence Detection Of Transgenic Arabidopsismentioning

confidence: 99%

MeGLYI-13, a Glyoxalase I Gene in Cassava, Enhances the Tolerance of Yeast and Arabidopsis to Zinc and Copper Stresses

Li,

Tang,

Che

et al. 2023

Plants

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Although zinc and copper are the two essential nutrients necessary for plant growth, their excessive accumulation in soil not only causes environmental pollution but also seriously threatens human health and inhibits plant growth. The breeding of plants with novel zinc or copper toxicity tolerance capacities represents one strategy to address this problem. Glyoxalase I (GLYI) family genes have previously been suggested to be involved in the resistance to a wide range of abiotic stresses, including those invoked by heavy metals. Here, a MeGLYI-13 gene cloned from a cassava SC8 cultivar was characterized with regard to its potential ability in resistance to zinc or copper stresses. Sequence alignment indicated that MeGLYI-13 exhibits sequence differences between genotypes. Transient expression analysis revealed the nuclear localization of MeGLYI-13. A nuclear localization signal (NLS) was found in its C-terminal region. There are 12 Zn2+ binding sites and 14 Cu2+ binding sites predicted by the MIB tool, of which six binding sites were shared by Zn2+ and Cu2+. The overexpression of MeGLYI-13 enhanced both the zinc and copper toxicity tolerances of transformed yeast cells and Arabidopsis seedlings. Taken together, our study shows the ability of the MeGLYI-13 gene to resist zinc and copper toxicity, which provides genetic resources for the future breeding of plants resistant to zinc and copper and potentially other heavy metals.

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Overexpression of Cassava MeAnn2 Enhances the Salt and IAA Tolerance of Transgenic Arabidopsis

Cited by 7 publications

References 54 publications

ATANN3 is involved in extracellular ATP-regulated auxin transport and distribution in Arabidopsis thaliana seedlings

ATANN3 is involved in extracellular ATP-regulated auxin transport and distribution in Arabidopsis thaliana seedlings

ATANN3 Is Involved in Extracellular ATP-Regulated Auxin Distribution in Arabidopsis thaliana Seedlings

MeGLYI-13, a Glyoxalase I Gene in Cassava, Enhances the Tolerance of Yeast and Arabidopsis to Zinc and Copper Stresses

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