A 4-bp Insertion at ZmPLA1 Encoding a Putative Phospholipase A Generates Haploid Induction in Maize

Liu, Chenxu; Li, Xiang; Meng, Dexuan; Zhong, Yu; Chen, Chen; Dong, Xin; Xu, Xiaowei; Chen, Baojian; Li, Wei; Li, Liang; Tian, Xiaolong; Zhao, Haiming; Song, Weibin; Luo, Haishan; Zhang, Qinghua; Lai, Jinsheng; Jin, Weiwei; Yan, Jianbing; Chen, Shaojiang

doi:10.1016/j.molp.2017.01.011

Cited by 242 publications

(192 citation statements)

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“…This candidate gene was named NOT LIKE DAD ( NLD ) because the PK6 allele induces maternal embryos without paternal contribution. Parallel independent studies named the same gene MATRILINEAL ( MTL ) and Phospholipase A1 ( ZmPLA1 ) (Kelliher et al , ; Liu et al , ).…”

Section: Resultsmentioning

confidence: 99%

Loss of pollen‐specific phospholipase NOT LIKE DAD triggers gynogenesis in maize

et al. 2017

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Gynogenesis is an asexual mode of reproduction common to animals and plants, in which stimuli from the sperm cell trigger the development of the unfertilized egg cell into a haploid embryo. Fine mapping restricted a major maize QTL (quantitative trait locus) responsible for the aptitude of inducer lines to trigger gynogenesis to a zone containing a single gene () coding for a patatin-like phospholipase A. In all surveyed inducer lines, carries a 4-bp insertion leading to a predicted truncated protein. This frameshift mutation is responsible for haploid induction because complementation with wild-type abolishes the haploid induction capacity. Activity of the promoter is restricted to mature pollen and pollen tube. The translational NLD::citrine fusion protein likely localizes to the sperm cell plasma membrane. In roots, the truncated protein is no longer localized to the plasma membrane, contrary to the wild-type NLD protein. In conclusion, an intact pollen-specific phospholipase is required for successful sexual reproduction and its targeted disruption may allow establishing powerful haploid breeding tools in numerous crops.

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Section: Resultsmentioning

confidence: 99%

Loss of pollen‐specific phospholipase NOT LIKE DAD triggers gynogenesis in maize

et al. 2017

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“…When this publication was in production, three articles (Kelliher et al 2017; Gilles et al 2017; Liu et al 2017) were published about cloning the gene underlying qhir1 QTL that codes for a sperm specific phospholipase and triggers haploid induction.…”

Section: Resultsmentioning

confidence: 99%

Dissection of a major QTL qhir1 conferring maternal haploid induction ability in maize

et al. 2017

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Key messageAmong the qhir11 and qhir12 sub-regions of a major QTL qhir1, only qhir11 has significant effect on maternal haploid induction, segregation distortion and kernel abortion.AbstractIn vivo haploid induction in maize can be triggered in high frequencies by pollination with special genetic stocks called haploid inducers. Several genetic studies with segregating populations from non-inducer x inducer crosses identified a major QTL, qhir1, on chromosome 1.04 contributing to in vivo haploid induction. A recent Genome Wide Association Study using 51 inducers and 1482 non-inducers also identified two sub-regions within the qhir1 QTL region, named qhir11 and qhir12; qhir12 was proposed to be mandatory for haploid induction because the haplotype of qhir11 was also present in some non-inducers and putative candidate genes coding for DNA and amino acid binding proteins were identified in the qhir12 region. To characterize the effects of each sub-region of qhir1 on haploid induction rate, F2 recombinants segregating for one of the sub-regions and fixed for the other were identified in a cross between CML269 (non-inducer) and a tropicalized haploid inducer TAIL8. To quantify the haploid induction effects of qhir11 and qhir12, selfed progenies of recombinants between these sub-regions were genotyped. F3 plants homozygous for qhir11 and/or qhir12 were identified, and crossed to a ligueless tester to determine their haploid induction rates. The study revealed that only the qhir11 sub-region has a significant effect on haploid induction ability, besides causing significant segregation distortion and kernel abortion, traits that are strongly associated with maternal haploid induction. The results presented in this study can guide fine mapping efforts of qhir1 and in developing new inducers efficiently using marker assisted selection.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-017-2873-9) contains supplementary material, which is available to authorized users.

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“…Novel edits in MTL result in 6.7% haploid offspring. A 4‐bp (CGAG) insertion in the fourth exon of ZmPLA1 in CAU5 (a haploid inducer derived from Stock 6) compared to the B73 reference genome was shown to be the cause of the haploid induction phenotype using CRISPR/Cas9 gene editing (Liu et al ., ). In the ZmPLA1 knockout lines, the average HIR is approximately 2%, which is close to the HIR of stock 6, indicating that the ZmPLA1 knockout method can be used to create haploid inducers.…”

Section: Haploidization Via Chromosome Eliminationmentioning

confidence: 97%

Novel technologies in doubled haploid line development

Ren

Trampe

et al. 2017

Plant Biotechnology Journal

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118

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Summaryhaploid inducer line can be transferred (DH) technology can not only shorten the breeding process but also increase genetic gain. Haploid induction and subsequent genome doubling are the two main steps required for DH technology. Haploids have been generated through the culture of immature male and female gametophytes, and through inter‐ and intraspecific via chromosome elimination. Here, we focus on haploidization via chromosome elimination, especially the recent advances in centromere‐mediated haploidization. Once haploids have been induced, genome doubling is needed to produce DH lines. This study has proposed a new strategy to improve haploid genome doubling by combing haploids and minichromosome technology. With the progress in haploid induction and genome doubling methods, DH technology can facilitate reverse breeding, cytoplasmic male sterile (CMS) line production, gene stacking and a variety of other genetic analysis.

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A 4-bp Insertion at ZmPLA1 Encoding a Putative Phospholipase A Generates Haploid Induction in Maize

Cited by 242 publications

References 9 publications

Loss of pollen‐specific phospholipase NOT LIKE DAD triggers gynogenesis in maize

Loss of pollen‐specific phospholipase NOT LIKE DAD triggers gynogenesis in maize

Dissection of a major QTL qhir1 conferring maternal haploid induction ability in maize

Novel technologies in doubled haploid line development

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