Novel technologies in doubled haploid line development

Ren, Jiaojiao; Wu, Penghao; Trampe, Benjamin; Tian, Xiaolong; Lübberstedt, Thomas; Chen, Shaojiang

doi:10.1111/pbi.12805

Cited by 119 publications

(110 citation statements)

References 108 publications

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“…Other advantage of DHs in crop breeding is the increase of the genetic gain; DH plants are regenerated from individual microspores and therefore, due to meiotic recombination, they contain new gene combinations which represent new recombinant products of the parental genomes fixed in homozygous state. Besides these advantages, DHs can be combined with other breeding approaches like mutation or gene transformation, leading to greatly accelerate cultivar development (Prem et al, 2004;Gurushidze et al, 2014;Dwivedi et al, 2015;Ren et al, 2017;Rustgi et al, 2017). For all these reasons, microspore embryogenesis in vitro systems are widely used by plant nursery and seed companies to rapidly generate new isogenic lines for breeding programs.…”

Section: Introductionmentioning

confidence: 99%

“…In cereals, the rate of spontaneous diploidization is high, whereas in vegetables and trees it is much lower and chemical treatments with drugs, such as colchicine, oryzalin, amiprophosmethyl, trifluralin or pronamide, should be applied during DHs production (Jain et al, 1996a;Barnabás et al, 1999;Pintos et al, 2007;Murovec and Bohanec, 2012). The frequency of spontaneous genome doubling has been reported up to 40-50% in Brassica napus, 40% in maize, 60% in rice, and 90% in barley and rye (Castillo et al, 2009;Prem et al, 2012;Ren et al, 2017), with nuclear fusion as the main mechanism for spontaneous diplodization in cereal species (Kasha et al, 2001(Kasha et al, , 2005Testillano et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Microspore embryogenesis: targeting the determinant factors of stress-induced cell reprogramming for crop improvement

Testillano¹

2019

Journal of Experimental Botany

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Under stress, isolated microspores are reprogrammed in vitro towards embryogenesis, producing doubled haploid plants, useful biotechnological tools in plant breeding as a source of new genetic variability, fixed in homozygous plants in only one generation. Stress-induced cell death and low rates of cell reprogramming are major factors that reduce the process yield. Knowledge gained in recent years has revealed that microspore embryogenesis initiation and progression involve a complex network of factors, whose roles are not yet well understood. Autophagy and cell-death proteases are crucial players in the response to stress, while cell reprogramming and totipotency acquisition are regulated by hormonal and epigenetic mechanisms. Auxin biosynthesis, transport and action are required for microspore embryogenesis. Initial stages involve DNA hypomethylation, H3K9 demethylation, and H3/H4 acetylation. Cell wall remodelling, with pectin de-methylesterification and AGP expression, is necessary for embryo development. We will review recent findings regarding the determinant factors underlying stress-induced microspore embryogenesis, focusing on the role of autophagy, cell death, auxin, chromatin modifications, and cell wall. Recent reports show that treatments with small modulators of autophagy, proteases and epigenetic marks reduce cell death and enhance embryogenesis initiation in several crops, opening up new possibilities for improving in vitro embryo production in breeding programs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microspore embryogenesis: targeting the determinant factors of stress-induced cell reprogramming for crop improvement

Testillano¹

2019

Journal of Experimental Botany

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“…This is consistent with patterns observed in older allopolyploids that exhibit subgenome dominance -dominant subgenome retaining a greater number of genes (33) . Lastly, because the resynthesized B. napus lines were made with doubled haploids, each of the independent lines started out genetically identical (34) . This permitted us to examine and compare the establishment of subgenome dominance across independently derived polyploid lines without the added influence of allelic variation segregating between different lines.…”

Section: Introductionmentioning

confidence: 99%

Replaying the evolutionary tape to investigate subgenome dominance in allopolyploid Brassica napus

Bird

Niederhuth

et al. 2019

Preprint

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One of the parental diploid genomes (subgenomes) in an allopolyploid often exhibits higher gene expression levels compared to the other subgenome(s) in the nucleus. However, the genetic basis and deterministic fate of subgenome expression dominance remains poorly understood. We examined the establishment of subgenome expression dominance in six isogenic resynthesized Brassica napus (rapeseed) allopolyploid lines over the first ten generations, and uncovered consistent expression dominance patterns that were biased towards the Brassica oleracea 'C' subgenome across each of the independent lines and generations. The number and direction of gene dosage changes from homoeologous exchanges (HEs) was highly variable between lines and generations, however, we recovered HE hotspots overlapping with those in multiple natural B. napus cultivars. Additionally, we found a greater number of 'C' subgenome regions replacing 'A' subgenome regions among resynthesized lines with rapid reduction in pollen counts and viability. Furthermore, DNA methylation differences between subgenomes mirrored the observed gene expression bias towards the 'C' subgenome in all lines and generations. Gene-interaction network analysis indicated an enrichment for network interactions and several biological functions for 'C' subgenome biased pairs, but no enrichment was observed for 'A' subgenome biased pairs. These findings demonstrate that "replaying the evolutionary tape" in allopolyploids results in repeatable and predictable subgenome expression dominance patterns based on preexisting genetic differences among the parental species.

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“…However, challenges such as crop duration, photoperiod and temperature sensitivity hinder multiple generations per year. Double haploids (Ren et al 2017), in vitro culturing of immature embryos (Wang et al 2009), embryo rescue technique (Rizal et al 2014), simplified biotron technique (Tanaka et al 2016) and others have been used for rapid generation cycling in many crops, but no such successes have occurred in chickpea. The first report on rapid generation advancement in chickpea provided evidence for three generations per year in short-season environments (Gaur et al 2007).…”

Section: Speed Breeding For Fast-forwarding Genetic Gainsmentioning

confidence: 99%

Integrating genomics for chickpea improvement: achievements and opportunities

et al. 2020

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Key message Integration of genomic technologies with breeding efforts have been used in recent years for chickpea improvement. Modern breeding along with low cost genotyping platforms have potential to further accelerate chickpea improvement efforts. Abstract The implementation of novel breeding technologies is expected to contribute substantial improvements in crop productivity. While conventional breeding methods have led to development of more than 200 improved chickpea varieties in the past, still there is ample scope to increase productivity. It is predicted that integration of modern genomic resources with conventional breeding efforts will help in the delivery of climate-resilient chickpea varieties in comparatively less time. Recent advances in genomics tools and technologies have facilitated the generation of large-scale sequencing and genotyping data sets in chickpea. Combined analysis of high-resolution phenotypic and genetic data is paving the way for identifying genes and biological pathways associated with breeding-related traits. Genomics technologies have been used to develop diagnostic markers for use in marker-assisted backcrossing programmes, which have yielded several molecular breeding products in chickpea. We anticipate that a sequence-based holistic breeding approach, including the integration of functional omics, parental selection, forward breeding and genome-wide selection, will bring a paradigm shift in development of superior chickpea varieties. There is a need to integrate the knowledge generated by modern genomics technologies with molecular breeding efforts to bridge the genome-to-phenome gap. Here, we review recent advances that have led to new possibilities for developing and screening breeding populations, and provide strategies for enhancing the selection efficiency and accelerating the rate of genetic gain in chickpea.

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Novel technologies in doubled haploid line development

Cited by 119 publications

References 108 publications

Microspore embryogenesis: targeting the determinant factors of stress-induced cell reprogramming for crop improvement

Microspore embryogenesis: targeting the determinant factors of stress-induced cell reprogramming for crop improvement

Replaying the evolutionary tape to investigate subgenome dominance in allopolyploid Brassica napus

Integrating genomics for chickpea improvement: achievements and opportunities

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