Dissection of a major QTL qhir1 conferring maternal haploid induction ability in maize

Nair, Sudha; Molenaar, Willem S.; Melchinger, Albrecht E.; Prasanna, Boddupalli M.; Martinez, Leocadio; López, Luis Antonio Tamayo; Chaikam, Vijay

doi:10.1007/s00122-017-2873-9

Cited by 22 publications

(31 citation statements)

References 34 publications

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“…Ten ears were randomly selected from each entry in each replication. EnAR and EmAR were determined in each ear as described earlier ( Xu et al, 2013 ; Nair et al, 2017a ). For comparison of degree of EnAR and EmAR among the inducers with different levels of HIR (determined based on the lg testcrosses in AF16A), inducers were categorized into three classes, namely (a) inducers with low HIR (<5%), (b) inducers with moderate HIR (5–8%), and (c) inducers with high HIR (with >8% HIR).…”

Section: Methodsmentioning

confidence: 99%

“…Considering the time and resources involved in assessment of HIR, it would also be efficient to use any morphological traits known to be associated with high HIR to select plants or families that can lead to identification of inducers with high HIR. Recently it was shown that ears of individual plants and families with high HIR showed increased rates of endosperm and embryo abortion compared to plants or families with low or no HIR ( Prigge et al, 2012b ; Xu et al, 2013 ; Nair et al, 2017a ). It is also well known that haploid plants do occur in the progenies of inducers.…”

Section: Introductionmentioning

confidence: 99%

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Marker-Assisted Breeding of Improved Maternal Haploid Inducers in Maize for the Tropical/Subtropical Regions

et al. 2018

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For efficient production of doubled haploid (DH) lines in maize, maternal haploid inducer lines with high haploid induction rate (HIR) and good adaptation to the target environments is an important requirement. In this study, we present second-generation Tropically Adapted Inducer Lines (2GTAILs), developed using marker assisted selection (MAS) for qhir1, a QTL with a significant positive effect on HIR from the crosses between elite tropical maize inbreds and first generation Tropically Adapted Inducers Lines (TAILs). Evaluation of 2GTAILs for HIR and agronomic performance in the tropical and subtropical environments indicated superior performance of 2GTAILs over the TAILs for both HIR and agronomic performance, including plant vigor, delayed flowering, grain yield, and resistance to ear rots. One of the new inducers 2GTAIL006 showed an average HIR of 13.1% which is 48.9% higher than the average HIR of the TAILs. Several other 2GTAILs also showed higher HIR compared to the TAILs. While employing MAS for qhir1 QTL, we observed significant influence of the non-inducer parent on the positive effect of qhir1 QTL on HIR. The non-inducer parents that resulted in highest mean HIR in the early generation qhir1+ families also gave rise to highest numbers of candidate inducers, some of which showed transgressive segregation for HIR. The mean HIR of early generation qhir1+ families involving different non-inducer parents can potentially indicate recipient non-inducer parents that can result in progenies with high HIR. Our study also indicated that the HIR associated traits (endosperm abortion rate, embryo abortion rate, and proportion of haploid plants among the inducer plants) can be used to differentiate inducers vs. non-inducers but are not suitable for differentiating inducers with varying levels of haploid induction rates. We propose here an efficient methodology for developing haploid inducer lines combining MAS for qhir1 with HIR associated traits.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Marker-Assisted Breeding of Improved Maternal Haploid Inducers in Maize for the Tropical/Subtropical Regions

et al. 2018

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“…Out of two qhir1 sub-regions, the region containing qhir11 had a significant effect on the generation of maternal haploids independent of the qhir12 allele, whereas the “inducer” qhir12 allele did not cause haploid induction higher than the spontaneous rate observed in case of wild-type cobs. In addition, the region containing qhir11 was reported to display segregation distortion and kernel abortion, which are characteristic for haploidy inducer lines (Nair et al 2017 ).…”

Section: Generation Of Haploids Via Intraspecific Hybridizationmentioning

confidence: 99%

State-of-the-art and novel developments of in vivo haploid technologies

et al. 2018

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The ability to generate (doubled) haploid plants significantly accelerates the crop breeding process. Haploids have been induced mainly through the generation of plants from cultivated gametophic (haploid) cells and tissues, i.e., in vitro haploid technologies, or through the selective loss of a parental chromosome set upon inter- or intraspecific hybridization. Here, we focus our review on the mechanisms responsible for the in vivo formation of haploids in the context of inter- and intraspecific hybridization. The application of a modified CENH3 for uniparental genome elimination, the IG1 system used for paternal as well as the BBM-like and the patatin-like phospholipase essential for maternal haploidy induction are discussed in detail.

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“…Quantitative trait loci (QTLs) are phenotypically defined genomic regions that contribute to allelic variation for a biological trait (Kumar et al ., ). Genes with a major effect can be experimentally mapped and characterized by establishing correlation between quantitative trait value and allelic states at linked genetic markers (Witzig et al ., ; Martin et al ., ; Nair et al ., ). Wondji et al .…”

Section: Introductionmentioning

confidence: 97%

A cluster of CYP6 gene family associated with the major quantitative trait locus is responsible for the pyrethroid resistance in Culex pipiens pallen

Zou

Guo

Shen

et al. 2019

Insect Molecular Biology

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The emergence and rapid spread of insecticide resistance in several mosquito species has become a significant obstacle in management of mosquito‐borne diseases, including deltamethrin resistance in Culex pipiens pallens. Previous study identified a major deltamethrin resistance quantitative trait locus (DR‐6) that alone explained 62% of the genetic variance. In this study, the marker L4B1.102 and L4B1.175 associated with the DR‐6 were characterized. We searched for potential candidate genes in the flank region of two markers in the genome sequence and showed that a cluster of CYP6 cytochrome P450 genes (CYP6BB4, CYP6BB3, CYP6CC2, CYP6P14, CYP6BZ2, CYP6AA9, CYP6AA8, CYP6AA7) was in the vicinity of DR‐6. Significant differences in the expression of these P450s in the larval and adult stages were identified in the resistant strains compared with the susceptible strain. For CYP6AA9 and CYP6BB4, the correlation analysis showed a highly positive correlation between relative gene expression quantification and the resistance level in different strains. Knockdown of CYP6BB4 increased the sensitivity of mosquitoes to deltamethrin. We identified that the deltamethrin resistance was in a cluster of CYP6 genes in C. pipiens pallens, and CYP6BB4 may play a significant role in the development of deltamethrin resistance.

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Dissection of a major QTL qhir1 conferring maternal haploid induction ability in maize

Cited by 22 publications

References 34 publications

Marker-Assisted Breeding of Improved Maternal Haploid Inducers in Maize for the Tropical/Subtropical Regions

Marker-Assisted Breeding of Improved Maternal Haploid Inducers in Maize for the Tropical/Subtropical Regions

State-of-the-art and novel developments of in vivo haploid technologies

A cluster of CYP6 gene family associated with the major quantitative trait locus is responsible for the pyrethroid resistance in Culex pipiens pallen

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