5′-Anchored simple-sequence repeat primers are useful for analysing potato somatic hybrids

Matthews, David E.; McNicoll, Jean; Harding, Keith; Millam, S.

doi:10.1007/s002990050735

Cited by 23 publications

(18 citation statements)

References 10 publications

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“…Other ISSR primers were used and no one led to an intermediate pattern between parental ones. The primer sequences were chosen according to published data on solanaceous plant characterization (Prevost and Wilkinson, 1999;Matthews et al, 1999).…”

Section: Resultsmentioning

confidence: 99%

Somatic hybrids between potato Solanum tuberosum and wild species Solanum verneï exhibit a recombination in the plastome

Trabelsi

Gargouri-Bouzid

Vedel

et al. 2005

Plant Cell Tiss Organ Cult

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In the present survey, interspecific somatic protoplast fusion between a dihaploid cultivated potato Solanum L. tuberosum (BF15) and a wild species Solanum verneı¨(V3) has been performed using the PEG method. Five putative hybrids were first selected. Among them, only two were retained. DNA analysis, using flow cytometry, showed that the first hybrid (VB2) was dihaploid (2n=2x=24 chromosomes), whereas the second (VB1) was hexaploid (2n=6x=72 chromosomes). In the greenhouse, these putative hybrids showed that they were able of raising and producing large tubers but with a modified shape compared to parental ones. Furthermore, they harbored an intermediate leaf morphology compared to their parents. The hybrid nature of these plants was first confirmed according to their esterase and peroxidase isoenzyme patterns. The RAPD analysis of genomic DNA and microsatellite based amplification (I-SSR) showed that both clones VB1 and VB2 are asymmetric somatic hybrids. They seem to have eliminated the major part of V3 parental nucleus but not the totality. The analysis of chloroplast DNA suggests that both hybrid plastomes were the result of a recombination between parental ones.

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Section: Resultsmentioning

confidence: 99%

Somatic hybrids between potato Solanum tuberosum and wild species Solanum verneï exhibit a recombination in the plastome

Trabelsi

Gargouri-Bouzid

Vedel

et al. 2005

Plant Cell Tiss Organ Cult

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“…However, as a method that combines most of the advantages of microsatellites (SSRs) and amplified fragment length polymorphism (AFLP) to the universality of random amplified polymorphic DNA (RAPD), ISSR-PCR can regenerate highly polymorphic, but reproducible, band profiles (Reddy et al 2002). In recent years, ISSR analysis was carried out frequently on identifying true hybrids in the stage of seedling (such as Matthews et al 1999;Scarano et al 2002). The present results indicated that ISSR can rapidly and reliably confirm the hybridity of the offspring in two cross combinations at early seedling stage (Figs.…”

Section: Discussionmentioning

confidence: 99%

Production of interspecific hybrids between Lilium longiflorum and L. lophophorum var. linearifolium via ovule culture at early stage

et al. 2009

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Interspecific hybridization was carried out between Lilium longiflorum and L. lophophorum var. linearifolium by using the cut style method of pollination, as a contrast, intraspecific hybridization between L. longiflorum 'Gelria' and L. longiflorum was also made, but no mature seeds and offspring were obtained from the two combinations under in vivo condition. Ovules excised from each carpel 5-35 days after pollination (DAP) were cultured on B5 or half-strength B5 medium containing sucrose at different concentrations in vitro. In L. longiflorum 9 L. lophophorum var. linearifolium, only 1.17% of ovules excised at 10 DAP developed into seedlings, and in L. longiflorum 'Gelria' 9 L. longiflorum, only 0.99% of ovules excised at 25 DAP developed into seedlings; none of the ovules excised at other different DAP in the two cross combinations produced any seedlings. The results showed that interspecific hybridization had a more serious post-fertilization barrier than the intraspecific hybridization, and that a lower concentration (3%) of sucrose led to better embryo development and higher percentage of seedlings in ovule cultures. All hybrid seedlings obtained were successfully transplanted to soil and grew normally. The progenies investigated were identified as true hybrids based on inter-simple sequence repeat (ISSR) analysis.

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“…O marcador molecular ISSR tem se mostrado uma poderosa ferramenta para análise da diversidade genética, bem como para a caracterização de acessos e cultivares de diversas espécies, como o cacau e espécies do gênero Solanum (Charters & Wilkinson, 2000;Isshiki et al, 2008). Por se tratar de um marcador multiloco que não requer conhecimento prévio do DNA a ser avaliado (Gupta et al, 1994), o ISSR é uma técnica de baixo custo, de fácil uso e de grande reprodutibilidade (Matthews et al, 1999). O ISSR utiliza uma sequência simples repetida como oligonucleotídeo iniciador (Olii) para amplificar um fragmento de DNA delimitado por dois microssatélites invertidos, o que gera um alto nível de polimorfismo.…”

Section: Introductionunclassified

Variabilidade genética entre acessos do gênero Manihot por meio de marcadores moleculares ISSR

Silva

Alves²,

Martins

et al. 2011

Pesq. agropec. bras.

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Genetic variation among accessions of the genus Manihot by ISSR markersAbstract -The objective of this work was to evaluate the genetic diversity within and among accessions of Manihot by using ISSR markers. Five species and two varieties of Manihot, besides two species of the genus Croton, used as the out-group, were analyzed by using 20 oligonucleotide (Olii) ISSR UBC primers. To analyze the similarity index between species and accessions, the Jaccard and simple matching coefficients were used. The 20 Olii tested were highly polymorphic in all species analyzed, and 89.7% of the loci were polymorphic. A higher genetic variability is observed among different species of Manihot, such as M. dichotoma var. undulata and M. caerulescens, than among individuals of the same species, such as M. dichotoma and M. dichotoma var. undulata.

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5′-Anchored simple-sequence repeat primers are useful for analysing potato somatic hybrids

Cited by 23 publications

References 10 publications

Somatic hybrids between potato Solanum tuberosum and wild species Solanum verneï exhibit a recombination in the plastome

Somatic hybrids between potato Solanum tuberosum and wild species Solanum verneï exhibit a recombination in the plastome

Production of interspecific hybrids between Lilium longiflorum and L. lophophorum var. linearifolium via ovule culture at early stage

Variabilidade genética entre acessos do gênero Manihot por meio de marcadores moleculares ISSR

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