In the present survey, interspecific somatic protoplast fusion between a dihaploid cultivated potato Solanum L. tuberosum (BF15) and a wild species Solanum verneı¨(V3) has been performed using the PEG method. Five putative hybrids were first selected. Among them, only two were retained. DNA analysis, using flow cytometry, showed that the first hybrid (VB2) was dihaploid (2n=2x=24 chromosomes), whereas the second (VB1) was hexaploid (2n=6x=72 chromosomes). In the greenhouse, these putative hybrids showed that they were able of raising and producing large tubers but with a modified shape compared to parental ones. Furthermore, they harbored an intermediate leaf morphology compared to their parents. The hybrid nature of these plants was first confirmed according to their esterase and peroxidase isoenzyme patterns. The RAPD analysis of genomic DNA and microsatellite based amplification (I-SSR) showed that both clones VB1 and VB2 are asymmetric somatic hybrids. They seem to have eliminated the major part of V3 parental nucleus but not the totality. The analysis of chloroplast DNA suggests that both hybrid plastomes were the result of a recombination between parental ones.
In this paper, we extend the Kirchhoff-Love model to thermal buckling and post-buckling analysis of functionally graded structures. The kernel idea of the proposed model consists of the consideration of large displacements and finite rotation to accurately model the thermal effects on buckling and post-buckling behavior of such structures. Both uniform and nonuniform temperature distributions are considered. Material properties of the FG structures are graded in the thickness direction and assumed to obey a power law distribution of the volume fraction of the constituents. The effectiveness and usefulness of the proposed model are highlighted through different numerical examples, and the effects of the volume fraction exponent, thermal loads, length-to-thickness ratio, boundary conditions and geometrical parameters on the buckling and post-buckling behavior of FGM structures are also examined.
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