Somatic hybrids between potato Solanum tuberosum and wild species Solanum verneï exhibit a recombination in the plastome

Abstract

In the present survey, interspecific somatic protoplast fusion between a dihaploid cultivated potato Solanum L. tuberosum (BF15) and a wild species Solanum verneı¨(V3) has been performed using the PEG method. Five putative hybrids were first selected. Among them, only two were retained. DNA analysis, using flow cytometry, showed that the first hybrid (VB2) was dihaploid (2n=2x=24 chromosomes), whereas the second (VB1) was hexaploid (2n=6x=72 chromosomes). In the greenhouse, these putative hybrids showed that they were able of raising and producing large tubers but with a modified shape compared to parental ones. Furthermore, they harbored an intermediate leaf morphology compared to their parents. The hybrid nature of these plants was first confirmed according to their esterase and peroxidase isoenzyme patterns. The RAPD analysis of genomic DNA and microsatellite based amplification (I-SSR) showed that both clones VB1 and VB2 are asymmetric somatic hybrids. They seem to have eliminated the major part of V3 parental nucleus but not the totality. The analysis of chloroplast DNA suggests that both hybrid plastomes were the result of a recombination between parental ones.

Progress in plant protoplast research

“…In Triticum aestivum + Setaria italica hybrids, cpDNA coexistence as well as recombination occur . It was also observed in Solanum tuberosum + verneï (Trabelsi et al 2005), Solanum berthaultii + tuberosum (Bidani et al 2007) and Bupleurum + Swertia (Jiang et al 2012).…”

“…GISH could also be used for monitoring genomic stabilization. If chromosome elimination occurs preferentially rather than randomly, e.g., in Solanum (Trabelsi et al 2005), it is a convenient tool to evaluate the effects of fusion and regeneration related parameters, and thus to optimize these parameters in an effort to stimulate or impede regeneration of particular genome types. A particular possible application of cytogenetic tools is the study of meiosis after somatic fusion to define chromosome affinity and phylogenetic relationship between the original fusion partners.…”

“…Intergenomic chloroplast recombination occurs rarely in higher plants, as opposed to the high level of mitochondrial recombination (Trabelsi et al 2005). The latter occurs after various fusions Iovene et al 2007;Yamagishi et al 2008).…”

Protoplast fusion in the genus Gentiana: genomic composition and genetic stability of somatic hybrids between Gentiana kurroo Royle and G. cruciata L.

“…These markers include the most popular, namely, RAPD (Xu et al 1993;De Filippis et al 1996;Szczerbakowa et al 2003;Guan et al 2010), microsatellites SSRs (Fu et al 2003;Cappelle et al 2007;Dambier et al 2011), AFLP (Brewer et al 1999;Guo et al 2002;Fu et al 2004;Tu et al 2008), ISSR (Scarano et al 2002;Trabelsi et al 2005;Xiao et al 2009) and, more recently, DArT markers (Smyda-Dajmund et al 2016). …”

Híbridos somáticos obtenidos por fusión de protoplastos entre Solanum tuberosum L. subsp. tuberosum y la especie silvestre Solanum circaeifolium Bitter

“…This can be done via morphological, biochemical, cytological and molecular markers (Pinto et al, 1995), and increasingly sophisticated molecular discrimination methods have recently been reported (Provan et al, 1996;Matthews et al, 1999;Harding and Millam, 2000;Trabelsi et al, 2005;Guo et al, 2007).…”