3D microextrusion-inkjet hybrid printing of structured human skin equivalents

Lee, Hwa‐Rim; Park, Ju An; Kim, Seongju; Jo, Youngmin; Kang, Dayoon; Jung, Sungjune

doi:10.1016/j.bprint.2021.e00143

Cited by 21 publications

(11 citation statements)

References 37 publications

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“…Nowadays, in parallel with the advance of technology, direct printing of living cells and biomaterials have opened up new possibilities for 3D tissue engineering and regenerative medicine [ 36 ]. The final product for the 3D-bioscaffolds is in the form of a hydrogel.…”

Section: 3d-bioprinting For Chronic Woundmentioning

confidence: 99%

Cellular Interaction of Human Skin Cells towards Natural Bioink via 3D-Bioprinting Technologies for Chronic Wound: A Comprehensive Review

Masri

Mazlan

Zulkiflee

et al. 2022

IJMS

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Skin substitutes can provide a temporary or permanent treatment option for chronic wounds. The selection of skin substitutes depends on several factors, including the type of wound and its severity. Full-thickness skin grafts (SGs) require a well-vascularised bed and sometimes will lead to contraction and scarring formation. Besides, donor sites for full-thickness skin grafts are very limited if the wound area is big, and it has been proven to have the lowest survival rate compared to thick- and thin-split thickness. Tissue engineering technology has introduced new advanced strategies since the last decades to fabricate the composite scaffold via the 3D-bioprinting approach as a tissue replacement strategy. Considering the current global donor shortage for autologous split-thickness skin graft (ASSG), skin 3D-bioprinting has emerged as a potential alternative to replace the ASSG treatment. The three-dimensional (3D)-bioprinting technique yields scaffold fabrication with the combination of biomaterials and cells to form bioinks. Thus, the essential key factor for success in 3D-bioprinting is selecting and developing suitable bioinks to maintain the mechanisms of cellular activity. This crucial stage is vital to mimic the native extracellular matrix (ECM) for the sustainability of cell viability before tissue regeneration. This comprehensive review outlined the application of the 3D-bioprinting technique to develop skin tissue regeneration. The cell viability of human skin cells, dermal fibroblasts (DFs), and keratinocytes (KCs) during in vitro testing has been further discussed prior to in vivo application. It is essential to ensure the printed tissue/organ constantly allows cellular activities, including cell proliferation rate and migration capacity. Therefore, 3D-bioprinting plays a vital role in developing a complex skin tissue structure for tissue replacement approach in future precision medicine.

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Section: 3d-bioprinting For Chronic Woundmentioning

confidence: 99%

Cellular Interaction of Human Skin Cells towards Natural Bioink via 3D-Bioprinting Technologies for Chronic Wound: A Comprehensive Review

Masri

Mazlan

Zulkiflee

et al. 2022

IJMS

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“…Significant progress in creating improved melanoma models has been made [ 76 ]. A repertoire of 3D in vitro models, such as spheroids [ 77 ] organoids [ 78 , 79 , 80 ], microfluidic platforms [ 81 ], human skin equivalents (HSEs) [ 50 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 ], and bioprinting [ 1 , 83 , 86 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 ] have been developed or have the potential to be used for the investigation of melanoma. Combinatorial strategies based on the principles of tissue engineering and bioprinting technologies are emerging as a viable route to creating biomimetic platforms for disease modelling.…”

Section: 3d In Vitro Models For Melanoma Modellingmentioning

confidence: 99%

“…This has been mainly motivated by the unprecedented ability of these technologies in depositing cells, biomaterials, and bioactive molecules in predefined 3D locations with high precision and reproducibility [ 145 , 146 , 147 ]. Bioprinted HSEs, exhibiting varying levels of biological function and complexity at structural, material, and cellular level, has been fabricated using different bioprinting technologies including vat polymerisation [ 148 , 149 ], inkjet [ 87 , 108 , 150 , 151 ], laser-assisted [ 152 , 153 ], and extrusion [ 84 , 87 , 88 , 97 , 107 , 116 , 154 ]. In vitro models of skin have experienced important advances as demonstrated by the bioprinting of multilayer tissue constructs comprising multiple cells, such as fibroblasts, endothelial cells, adipocytes, pericytes, stem cells, induced pluripotent stem cells (iPSCs), melanocytes, and keratinocytes [ 1 , 86 , 93 , 97 , 105 , 108 ].…”

Section: Bioprinting Skin and Melanoma Modelsmentioning

confidence: 99%

3D Bioprinting: An Enabling Technology to Understand Melanoma

Fernandes

Vyas

Lim

et al. 2022

Cancers

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Melanoma is a potentially fatal cancer with rising incidence over the last 50 years, associated with enhanced sun exposure and ultraviolet radiation. Its incidence is highest in people of European descent and the ageing population. There are multiple clinical and epidemiological variables affecting melanoma incidence and mortality, such as sex, ethnicity, UV exposure, anatomic site, and age. Although survival has improved in recent years due to advances in targeted and immunotherapies, new understanding of melanoma biology and disease progression is vital to improving clinical outcomes. Efforts to develop three-dimensional human skin equivalent models using biofabrication techniques, such as bioprinting, promise to deliver a better understanding of the complexity of melanoma and associated risk factors. These 3D skin models can be used as a platform for patient specific models and testing therapeutics.

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“…Preparation of Bioink for the Dermis Model. The dermal layer matrix was prepared by applying the microextrusionbased method, with slight modifications, to generate dermal equivalent, as described previously [33]. In brief, type I collagen (Dalim Tissen, Republic of Korea) was dissolved in 0.1% acetic acid to obtain a 0.75% (w/v) acidic aqueous solution.…”

Section: Immunoblot Analysismentioning

confidence: 99%

“…Immunohistochemical Analysis. Immunohistochemistry was carried out using cross-sectioned tissues as previously described [33]. Briefly, Aronia extract-treated 3D dermis models were dehydrated 5 min and then fixed in 4% paraformaldehyde solution (Biosesang, Republic of Korea).…”

Section: Compressive Modulus Of Aronia Extract-treatedmentioning

confidence: 99%

Effect of Aronia Extract on Collagen Synthesis in Human Skin Cell and Dermal Equivalent

Lee

Ryu

Lee

et al. 2022

Oxidative Medicine and Cellular Longevity

Self Cite

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The regulation of collagen synthesis, which occurs in fibroblasts in the dermal layer, is a key process in dermis regeneration and skin reconstruction. Herein, we investigated whether Aronia melanocarpa extract affects the human skin condition. We focused on type I collagen synthesis using two different types of model systems: a monolayer of cells and a bioprinted 3D dermal equivalent. The Aronia extract showed no cytotoxicity and increased cell proliferation in neonatal human dermal fibroblasts. Treatment with Aronia extract increased the transcription of COL1A1 mRNA in direct proportion to the extract concentration without causing a decrease in COL1A1 mRNA degradation. Additionally, the Aronia extract inhibited the expression of MMP1 and MMP3, and an increase in type I collagen was observed along with a decrease in MMP1 protein. We also fabricated dermal equivalents from type I collagen (the major component of the dermis) and dermal fibroblasts by bioprinting. In the 3D dermis model, the compressive modulus directly affected by collagen synthesis increased in direct proportion to the Aronia extract concentration, and expression levels of MMP1 and MMP3 decreased in exactly inverse proportion to its concentration. The findings that the Aronia extract increases synthesis of type I collagen and decreases MMP1 and MMP3 expression suggest that this extract may be useful for the treatment of damaged or aged skin.

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3D microextrusion-inkjet hybrid printing of structured human skin equivalents

Cited by 21 publications

References 37 publications

Cellular Interaction of Human Skin Cells towards Natural Bioink via 3D-Bioprinting Technologies for Chronic Wound: A Comprehensive Review

Cellular Interaction of Human Skin Cells towards Natural Bioink via 3D-Bioprinting Technologies for Chronic Wound: A Comprehensive Review

3D Bioprinting: An Enabling Technology to Understand Melanoma

Effect of Aronia Extract on Collagen Synthesis in Human Skin Cell and Dermal Equivalent

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