Effect of Aronia Extract on Collagen Synthesis in Human Skin Cell and Dermal Equivalent

Abstract

The regulation of collagen synthesis, which occurs in fibroblasts in the dermal layer, is a key process in dermis regeneration and skin reconstruction. Herein, we investigated whether Aronia melanocarpa extract affects the human skin condition. We focused on type I collagen synthesis using two different types of model systems: a monolayer of cells and a bioprinted 3D dermal equivalent. The Aronia extract showed no cytotoxicity and increased cell proliferation in neonatal human dermal fibroblasts. Treatment with Aronia extract increased the transcription of COL1A1 mRNA in direct proportion to the extract concentration without causing a decrease in COL1A1 mRNA degradation. Additionally, the Aronia extract inhibited the expression of MMP1 and MMP3, and an increase in type I collagen was observed along with a decrease in MMP1 protein. We also fabricated dermal equivalents from type I collagen (the major component of the dermis) and dermal fibroblasts by bioprinting. In the 3D dermis model, the compressive modulus directly affected by collagen synthesis increased in direct proportion to the Aronia extract concentration, and expression levels of MMP1 and MMP3 decreased in exactly inverse proportion to its concentration. The findings that the Aronia extract increases synthesis of type I collagen and decreases MMP1 and MMP3 expression suggest that this extract may be useful for the treatment of damaged or aged skin.

Inkjet-based bioprinting for tissue engineering

“…Their research, facilitated by the development of 3D skin models, revealed that the Aronia extract enhanced the synthesis of type I collagen and reduced the expression of MMP1 and MMP3. These findings suggest that this extract could be beneficial in treating damaged or aged skin [81].…”

Сок Черноплодной Рябины: 10 Веских Причин Добавить В Свой Рацион

“…Маски из данного богатого витамином C и прочими полезными микроэлементами продукта способствуют ускоренному синтезу коллагена в тканях и рекомендуются специалистами для безопасного и эффективного лечения стареющей и поврежденной дермы. [24] 10. Полезен для спортсменов-бегунов Бег на длинные дистанции может быть опасен для бегунов-любителей -повышенные кардионагрузки увеличивают риски остановки сердца за счет усиления активации тромбоцитов.…”

Consumption of Chokeberry Bio-Products Improves Specific Metabolic Parameters and Increases the Plasma Antioxidant Status

“…Additionally, it has been shown that the consumption of chokeberry juice may have hypotensive, lipid-lowering, and hypoglycemic effects, as well as reduce the levels of liver enzymes [ 18 , 19 , 20 ]. Studies on animals or cell cultures report chokeberry’s hepatoprotective [ 21 , 22 ], anti-inflammatory [ 23 , 24 , 25 ], neuroprotective [ 26 , 27 ], antidepressant [ 28 ], anti-aging [ 29 ], anticancer [ 30 ], antiviral [ 31 ], and antibacterial effects [ 32 ]. Because of their contents of valuable health-promoting ingredients, chokeberry products can be used both in the prevention and support of the treatment for existing metabolic disorders [ 18 ].…”