[35] Comparative studies on different methods of malonaldehyde determination

Bird, Ranjana P.; Draper, H. H.

doi:10.1016/s0076-6879(84)05038-2

Cited by 492 publications

(239 citation statements)

References 20 publications

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“…As diferenças foram estatisticamente significativas tanto entre o grupo total de pacientes com ARJ (p<0,0001), como entre os subgrupos de doença poliarticular (p<0,01), oligoarticular (p<0,01) e sistêmica (p<0,0001), quando comparados aos controles (Tabela 3). O malondialdeído (MDA) é um produto gerado na oxigenação enzimática do ácido araquidônico e um produto da degradação oxidativa dos lipídios 21,22 . Em nossos pacientes, a concentração plasmática do MDA foi significativamente mais elevada quando comparada aos controles.…”

Section: Resultsunclassified

The role of oxidative stress in inflammation in patients with juvenile rheumatoid arthritis

Ramos¹,

Ramos²,

Dominguez³

2000

J Pediatr (Rio J)

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Objective: To determine the level of cellular oxidative stress blood markers and the enzymatic system of antioxidant defense establishing the oxidative profile in patients with Juvenile Rheumatoid Arthritis.Methods: Case-control study that included 64 patients (46 of female sex) with Juvenile Rheumatoid Arthritis (JRA) following clinical control in the Pediatric Rheumatology Service of the Vall d'Hebron Hospital, Barcelona, Spain. The patients were separated in three subtypes based on the pattern of onset within the first six months of disease: polyarticular, pauciarticular and systemic. The control group included 60 patients (38 of female sex) following clinical control to diseases of non inflammatory nature, in the same hospital. The plasmatic levels of malondialdehyde (MDA), lipoperoxide (LPO), hydroperoxide (HPX), carbonile groups (CG) of proteins and gluthathione and the enzymatic activities of Superoxide dismutase (SOD), gluthathione peroxidase (GSH-Px) and gluthathione reductase were determined.Results: The group of patients with JRA presented high concentrations of lipid peroxidation products, evaluated by determining the plasmatic levels of MDA, LPO, and HPX; oxidative damage of the circulate protein, determined by CG contents of plasma proteins; elevation of enzymatic activity of SOD and GSH-Red; decrease of GSH-Px activity and GSH levels.Conclusions: Our results show the presence of molecular damage determined by oxygen free radicals in the JRA patients. The SOD activity and the changes of gluthathione redox enzymatic cycle confirm the decrease of capacity of cellular defense system against the induced toxicity of oxidative stress in these patients. Resultados: Comparados aos controles, os pacientes portadores de ARJ apresentaram concentrações elevadas dos produtos derivados da lipoperoxidação, avaliada pela determinação dos níveis plasmáticos do MDA, HPX e LPO; dano oxidativo das proteínas circulantes, determinado pelo conteúdo de GC de proteínas plasmá-ticas; elevação da atividade enzimática da SOD e GSH-Red; e diminuição da atividade da GSH-Px e dos níveis do GSH. J. pediatr. (Rio J.Conclusões: Estes resultados demonstram a presença de dano molecular determinado pelos radicais livres de oxigênio em pacientes com ARJ. A atividade da SOD e as alterações do ciclo enzimático glutatião-redox confirmam uma diminuição da capacidade dos sistemas de defesa intracelular contra a toxicidade induzida pelo estresse oxidativo nestes pacientes. Introdução A geração de radicais livres ocorre como parte do processo fisiológico do metabolismo aeróbico, secundária à produção de radicais reativos de oxigênio. Desse modo, o metabolismo celular produz radicais livres em condições fisiológicas, e estes radicais ativos podem ter vários efeitos J. pediatr. (Rio J.

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Section: Resultsunclassified

The role of oxidative stress in inflammation in patients with juvenile rheumatoid arthritis

Ramos¹,

Ramos²,

Dominguez³

2000

J Pediatr (Rio J)

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“…Serum Malondialdehyde (MDA) and serum protein carbonyls measured by thibarbituric corrosive receptive substance assay (TBARS) and ELISA pack technique 13 . Serum superoxide dismutase (SOD) was measured by hindrance of auto oxidation of adrenaline by superoxide dismutase method and serum catalase was measured by goth's method 14,15 .…”

Section: Biochemical Assaysmentioning

confidence: 99%

Pro oxidants and Antioxidants Levels in Chronic Renal Failure Patients Treated by Dialysis

Kundoor

Mohanty

Narsini

et al. 2017

AJPRHC

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To assess adjustments in star oxidant and cancer prevention agent state in pre and post hemodialysis patients experiencing ceaseless renal disease. The study was led on 100 perpetual renal disappointment patients going to outpatient division of nephrology dialysis unit in relationship with bureau of organic chemistry at Kamineni establishment of therapeutic Sciences, Narketpally, Nalgonda district, Telangana. Age between 30-70 years of either sex measuring their estimated oxidant levels of serum malondialdehyde, serum protein carbonyls and anti-oxidant levels of serum superoxide dismutase, serum catalase prior and then afterward the dialysis session coordinated controls. In the present study, we have observed significant increase in levels of pro-oxidants serum malondialdehyde and serum protein carbonyls fixation and there was reduction in chemical action of cell reinforcement serum superoxide dismutase and serum catalase. Our study highlights the need of screening of cancerous agents in renal Patients in regular intervals for better treatment outcomes and also to improve quality of life of the patients.

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“…The thiobarbituric acid reactive substances (TBARS) were determined as described elsewhere (Bird and Draper 1984). White chocolate samples (5 g) were placed in a 50-ml flask, dissolved in chloroform and transferred to 100 ml volumetric flask and the volume was completed with chloroform.…”

Section: Thiobarbituric Acid Reactive Substances (Tbars)mentioning

confidence: 99%

Changes in the color of white chocolate during storage: potential roles of lipid oxidation and non-enzymatic browning reactions

2011

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Three different samples of white chocolate were prepared: a sample with a synthetic antioxidant, another with casein peptides as natural antioxidant, and a third sample without any kind of antioxidant. Parameters associated with lipid oxidation and non-enzymatic browning were evaluated in the different samples of white chocolate during 10 months storage at 20 and 28°C. Acidity, thiobarbituric acid reactive substances and peroxide values increased with the incubation time. Samples stored at 20°C often showed lower values for these parameters than those stored at 28°C, although the differences were not always significant. The values for water activity increased from 0.4 to 0.53-0.57 during the period of 10 months. The color parameter a* was increased in samples stored at 28°C from month 5, and the parameter b* was lower in samples containing antioxidants from month 2. The addition of antioxidants did not significantly influence most the parameters studied, suggesting that the main parameters governing the alterations of white chocolate during its shelf-life was the storage temperature and increase in water activity.

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[35] Comparative studies on different methods of malonaldehyde determination

Cited by 492 publications

References 20 publications

The role of oxidative stress in inflammation in patients with juvenile rheumatoid arthritis

The role of oxidative stress in inflammation in patients with juvenile rheumatoid arthritis

Pro oxidants and Antioxidants Levels in Chronic Renal Failure Patients Treated by Dialysis

Changes in the color of white chocolate during storage: potential roles of lipid oxidation and non-enzymatic browning reactions

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