Quercetin Stimulates Bone Marrow Mesenchymal Stem Cell Differentiation through an Estrogen Receptor-Mediated Pathway

Pang, Xin-Gang; Cong, Yu; Bao, Nirong; Li, Yonggang; Zhao, Jianning

doi:10.1155/2018/4178021

Cited by 59 publications

(45 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…20 Another study showed that quercetin enhanced bone marrow MSC proliferation and osteogenic differentiation. 21 This indicates that C. olitorius has the potential to increase stem cell proliferation by its rich polyphenolic content, which might be supportive for stem cell differentiation and better for mimicking in vivo structures and further tissue repair.…”

Section: Discussionmentioning

confidence: 99%

Obtaining Stem Cell Spheroids from Foreskin Tissue and the Effect of <i>Corchorus olitorius</i> L. on Spheroid Proliferation

Becer¹,

Soykut²,

Kabadayı³

et al. 2020

tjps

View full text Add to dashboard Cite

Mezenkimal kök hücreler kendi kendini yenileyebilme özelliğine sahiptir. Sünnet derisinin kök hücre kaynağı olarak kullanılma potansiyeli vardır. Çalışmanın amacı, yeni doğmuş insan sünnet derisi izole hücrelerinden (hnFSSCs) sferoid oluşumunu elde etmektir. Buna ek olarak, Kıbrıs'a özgü geleneksel bir bitki olan Corchorus olitorius L. (C. olitorius) bitkisinin hnFSSCs sferoidleri üzerindeki apoptotik ve proliferatif etkileri de araştırılmıştır. Gereç ve Yöntemler: Rutin sünnet prosedüründen sonra hücreler izole edildi ve uygun besi yeri ortamında kültüre edildi. Bitki yaprakları etanol ile ekstre edildi ve içerik analizi sıvı kromatografi-kütle spektrometresi (LC-MS/MS) yöntemi ile yapıldı. Sünnet derisi kök hücreleri CD45, CD34, CD90 antikorları kullanılarak immünositokimyasal olarak karakterize edilmiştir. hnFSSC sferoidleri asılı damla tekniği kullanılarak oluşturuldu. Elde edilen sferoidler daha sonra C. olitorius ekstraktı ile 48 saat süre muamele edildikten sonra kaspaz-3 ve Ki-67'nin dağıtımı için immünofloresan boyama yöntemiyle boyandı. Objectives: Mesenchymal stem cells are self-renewing stem cells. The human foreskin has potential to be used as a source of stem cells. The aim of the study was to obtain spheroid formation of human foreskin cells (hnFSSCs) isolated from newborn human foreskin tissue. In addition, the apoptotic and proliferative effects of a traditional plant, Corchorus olitorius L. (C. olitorius), on hnFSSC spheroids were investigated. Materials and Methods: After a routine circumcision procedure the cells were isolated and cultured in suitable medium. The plant leaves was extracted with ethanol and their composition was analyzed by liquid chromatography coupled with mass spectrometry (LC-MS/MS). The foreskin stem cells were characterized immunocytochemically by CD45, CD34, and CD90 antibodies. hnFSSC spheroids were formed using the hanging drop technique. Immunofluorescence staining was used on the obtained spheroids to determine the distribution of caspase-3 and Ki-67 after being treated with C. olitorius extract for 48 h. Results: Immunostaining analysis showed that hnFSSCs were positive for CD45 and CD34 and negative for CD90. According to LC-MS/MS C. olitorius was rich in flavanols and hydrocinnamic acid derivatives. Although the spheroids obtained were loose and floating, the cells interacted with each other. Caspase-3 activity was higher in the control group than in the extract-treated group and Ki-67 was higher in the extract-treated group than in the control group, suggesting that the plant might have the capacity to increase stem cell proliferation due to its rich polyphenolic content. Conclusion: The results suggest that hnFSSCs and spheroids might be used in stem cell generation, tissue repair and renewal as human foreskin tissue has potential to be used as a stem cell source. C. olitorius also increased proliferation of hnFSSCs, showing that polyphenols might increase proliferation of stem cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Obtaining Stem Cell Spheroids from Foreskin Tissue and the Effect of <i>Corchorus olitorius</i> L. on Spheroid Proliferation

Becer¹,

Soykut²,

Kabadayı³

et al. 2020

tjps

View full text Add to dashboard Cite

show abstract

“…In spleen and bone marrow, the expression was increased significantly, while in the liver, it decreased significantly (Figure 9). Modulation of Higd2a expression might be related to the effects of quercetin on cellular proliferation in promoting healthy bone marrow mesenchymal stem cell (BMSC) proliferation [66,67]. BMSCs cultured and treated with quercetin (0.1-5 µM and 1-10 µM for the isolation of mouse and rat tissues, respectively) significantly stimulated cells [66,67].…”

Section: Discussionmentioning

confidence: 99%

“…Modulation of Higd2a expression might be related to the effects of quercetin on cellular proliferation in promoting healthy bone marrow mesenchymal stem cell (BMSC) proliferation [66,67]. BMSCs cultured and treated with quercetin (0.1-5 µM and 1-10 µM for the isolation of mouse and rat tissues, respectively) significantly stimulated cells [66,67]. On the other hand, quercetin could have antiproliferative effects [68][69][70][71].…”

Section: Discussionmentioning

confidence: 99%

Biosystem Analysis of the Hypoxia Inducible Domain Family Member 2A: Implications in Cancer Biology

Salazar

Yáñez

Elorza

et al. 2020

Genes

View full text Add to dashboard Cite

The expression of HIGD2A is dependent on oxygen levels, glucose concentration, and cell cycle progression. This gene encodes for protein HIG2A, found in mitochondria and the nucleus, promoting cell survival in hypoxic conditions. The genomic location of HIGD2A is in chromosome 5q35.2, where several chromosomal abnormalities are related to numerous cancers. The analysis of high definition expression profiles of HIGD2A suggests a role for HIG2A in cancer biology. Accordingly, the research objective was to perform a molecular biosystem analysis of HIGD2A aiming to discover HIG2A implications in cancer biology. For this purpose, public databases such as SWISS-MODEL protein structure homology-modelling server, Catalogue of Somatic Mutations in Cancer (COSMIC), Gene Expression Omnibus (GEO), MethHC: a database of DNA methylation and gene expression in human cancer, and microRNA-target interactions database (miRTarBase) were accessed. We also evaluated, by using Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR), the expression of Higd2a gene in healthy bone marrow-liver-spleen tissues of mice after quercetin (50 mg/kg) treatment. Thus, among the structural features of HIG2A protein that may participate in HIG2A translocation to the nucleus are an importin α-dependent nuclear localization signal (NLS), a motif of DNA binding residues and a probable SUMOylating residue. HIGD2A gene is not implicated in cancer via mutation. In addition, DNA methylation and mRNA expression of HIGD2A gene present significant alterations in several cancers; HIGD2A gene showed significant higher expression in Diffuse Large B-cell Lymphoma (DLBCL). Hypoxic tissues characterize the “bone marrow-liver-spleen” DLBCL type. The relative quantification, by using qRT-PCR, showed that Higd2a expression is higher in bone marrow than in the liver or spleen. In addition, it was observed that quercetin modulated the expression of Higd2a gene in mice. As an assembly factor of mitochondrial respirasomes, HIG2A might be unexpectedly involved in the change of cellular energetics happening in cancer. As a result, it is worth continuing to explore the role of HIGD2A in cancer biology.

show abstract

“…Apigenin and luteolin promoted human hematopoietic stem cell differentiation [141] and were confirmed safe for developing vertebrates in terms of neurobehavior [142]. [138] into bone cells Increased Rat BM-MSCs cultures [129], mice BM-MSC cultures [130] Decreased Human BM cell cultures [133] into β-cells Increased Rat BM-MSCs cultures [131] into hepatic cells Increased Human BM cell cultures [134] into fat tissue cells Increased Human BM cell cultures [133] Proinflammatory cytokines Decreased Rat model of spinal cord injury [132] Anti-inflammatory cytokines Increased Rat model of spinal cord injury [132] Angiogenic factor secretion Increased Rat BM-MSC cultures [135] BDNF secretion Increased Human embryonic NSCs culture [138] BDNF: Brain-derived neurotrophic factor, BM: Bone marrow, hHSC: Human hematopoietic stem cells, MSC: Mesenchymal stem cells, NSC: Neural stem cells, hPSCs: Human pluripotent stem cells.…”

Section: Past and Future Perspectivesmentioning

confidence: 96%

“…Quercetin has multiple effects (Table 4). It enhanced the differentiation of BM-MSCs into bone cells in rat [129] and mice models [130]. In vitro, quercetin promoted the differentiation of BM-MSCs into β-cells with increased insulin secretion [131].…”

Section: Past and Future Perspectivesmentioning

confidence: 99%

Autologous Cord Blood in Children with Cerebral Palsy: A Review

Boruczkowski

Pujal²,

Zdolińska-Malinowska

2019

IJMS

View full text Add to dashboard Cite

The aim of this narrative review is to report on the current knowledge regarding the clinical use of umbilical cord blood (CB) based on articles from PubMed and clinical trials registered on ClinicalTrials.gov. An increasing amount of evidence suggests that CB may be used for both early diagnostics and treatment of cerebral palsy. The acidity of CB and its biochemical parameters, including dozens of cytokines, growth factors, and other metabolites (such as amino acids, acylcarnitines, phosphatidylcholines, succinate, glycerol, 3-hydroxybutyrate, and O-phosphocholine) are predictors of future neurodevelopment. In addition, several clinical studies confirmed the safety and efficacy of CB administration in both autologous and allogeneic models, including a meta-analysis of five clinical trials involving a total of 328 participants. Currently, nine clinical trials assessing the use of autologous umbilical CB in children diagnosed with hypoxic-ischemic encephalopathy or cerebral palsy are in progress. The total population assessed in these trials exceeds 2500 patients.

show abstract

Quercetin Stimulates Bone Marrow Mesenchymal Stem Cell Differentiation through an Estrogen Receptor-Mediated Pathway

Cited by 59 publications

References 51 publications

Obtaining Stem Cell Spheroids from Foreskin Tissue and the Effect of <i>Corchorus olitorius</i> L. on Spheroid Proliferation

Obtaining Stem Cell Spheroids from Foreskin Tissue and the Effect of <i>Corchorus olitorius</i> L. on Spheroid Proliferation

Biosystem Analysis of the Hypoxia Inducible Domain Family Member 2A: Implications in Cancer Biology

Autologous Cord Blood in Children with Cerebral Palsy: A Review

Contact Info

Product

Resources

About