Obtaining Stem Cell Spheroids from Foreskin Tissue and the Effect of &lt;i&gt;Corchorus olitorius&lt;/i&gt; L. on Spheroid Proliferation

Becer, Eda; Soykut, Günsu; Kabadayı, Hilal; Mammadov, Emil; Çalış, İhsan; Vatansever, Hafize Seda

doi:10.4274/tjps.galenos.2019.05658

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…It also acts as an important pathogenic/diagnostic model of microorganisms and as a model for the examination of medications [24]. Several studies revealed that human foreskin cells could generate stem cells for tissue repair and renewal, and for the treatment of diseases [11,25,26]. Willingness to donate tissue for research has been examined by several studies from different parts of the world.…”

Section: Discussionmentioning

confidence: 99%

Attitude towards donation of the excised foreskin after circumcision surgery for research: A study from Madinah, Saudi Arabia

Khabour,

Mahallawi,

Ali

et al. 2023

PLoS ONE

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Studies have shown the possibility of using the part of the foreskin removed after circumcision in the field of scientific and therapeutic research. Donations of tissues and organs are always associated with ethical challenges posed by bioethicists and societies to ensure the appropriate use of these tissues/organs. The purpose of this study was to understand the attitudes and awareness of parents/guardians regarding donation of excised foreskin to research and medical use. The study was based on a questionnaire and included 133 parents/guardians who visited Uhud Children’s Hospital in Madinah, Saudi Arabia for newborn male circumcision. The results showed a high willingness (61.7%) to donate the extracted foreskin to research. The willingness to donate the extracted foreskin to research associated with undergraduate degree (P = 0.018), male sex (P = 0.011), high income (P = 0.029), and participation in previous research studies (P = 0.002). About 41.8% were convinced that written informed consent should be obtained before circumcision surgery, 38.1% (n = 51) were convinced that written informed consent should be taken after surgery, while the remaining 19.4% reported that the timing of written informed consent is unimportant. Finally, fear of excision of excess tissue (74.5%), lack of confidence in the research (68.6%), and potential for commercial use (64.7%) were the main barriers to unwillingness to donate the excised foreskin for research. In conclusion, a reasonable portion of Saudis agreed to donate their foreskin for research purposes. There is an urgent need to enhance awareness and attitudes towards tissue donation for research and therapeutic use.

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Section: Discussionmentioning

confidence: 99%

Attitude towards donation of the excised foreskin after circumcision surgery for research: A study from Madinah, Saudi Arabia

Khabour,

Mahallawi,

Ali

et al. 2023

PLoS ONE

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“…The extract was evaporated and lyophilized (Christ Alpha 1-4 LD Plus, Germany) and the final yield of crude extract was found to be 14.8 grams. Extraction composition, LC-MS/MS analysis was done by Becer et al (2020).…”

Section: Extractionmentioning

confidence: 99%

The Effect of Corchorus olitorius L. Extract on Viability of Breast Cancer Cells: A friend or a foe?

Soykut¹,

Becer²,

Çalış³

et al. 2019

Current Perspectives on Medicinal and Aromatic Plants (CUPMAP)

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This Corchorus olitorius L. is known to be a medicinal plant widely consumed in the Eastern Mediterranean region and has anti-bacterial, anti-inflammatory and anti-cancer properties. Breast cancer has one of the highest mortality rates among all cancers. Therefore, the main aim of the study is to evaluate the effectiveness of C. olitorius plant on viability of estrogen receptor (ER) positive breast cancer cell line, MCF-7. C. olitorius leaves first extracted with ethanol, then LC-MS/MS analysis was done for identification of phytochemicals. MTT assay was used for assessment of cell viability of MCF-7 cells. Cells were treated with C. olitorius extracts at five concentrations (5, 10, 20, 50, 100 µg/ml) and four different incubation periods (24, 48, 72, 96 h). LC-MS/MS analysis identified seven phytochemicals in the extract, mainly quercetin and caffeoylquinic acid derivatives. MTT results showed that the extract was only slightly effective in terms of reduction of cell viability at 50 and 100 µg/ml doses which were incubated for 24 and 48 h. Lower concentration doses did not show any effect in cell viability of MCF-7 cells. Longer incubation periods tend to increase cell viability of breast cancer cells. Quercetin identified within the extract might interfere with ER and promote MCF-7 cell proliferation. Therefore, in ER positive breast adenocarcinoma, quercetin intake and doses should carefully be monitored. More studies regarding the relationship between C. olitorius, quercetin and breast cancer should be done for further clarification of the topic.

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Obtaining Stem Cell Spheroids from Foreskin Tissue and the Effect of <i>Corchorus olitorius</i> L. on Spheroid Proliferation

Cited by 2 publications

References 19 publications

Attitude towards donation of the excised foreskin after circumcision surgery for research: A study from Madinah, Saudi Arabia

Attitude towards donation of the excised foreskin after circumcision surgery for research: A study from Madinah, Saudi Arabia

The Effect of Corchorus olitorius L. Extract on Viability of Breast Cancer Cells: A friend or a foe?

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