Mezenkimal kök hücreler kendi kendini yenileyebilme özelliğine sahiptir. Sünnet derisinin kök hücre kaynağı olarak kullanılma potansiyeli vardır. Çalışmanın amacı, yeni doğmuş insan sünnet derisi izole hücrelerinden (hnFSSCs) sferoid oluşumunu elde etmektir. Buna ek olarak, Kıbrıs'a özgü geleneksel bir bitki olan Corchorus olitorius L. (C. olitorius) bitkisinin hnFSSCs sferoidleri üzerindeki apoptotik ve proliferatif etkileri de araştırılmıştır. Gereç ve Yöntemler: Rutin sünnet prosedüründen sonra hücreler izole edildi ve uygun besi yeri ortamında kültüre edildi. Bitki yaprakları etanol ile ekstre edildi ve içerik analizi sıvı kromatografi-kütle spektrometresi (LC-MS/MS) yöntemi ile yapıldı. Sünnet derisi kök hücreleri CD45, CD34, CD90 antikorları kullanılarak immünositokimyasal olarak karakterize edilmiştir. hnFSSC sferoidleri asılı damla tekniği kullanılarak oluşturuldu. Elde edilen sferoidler daha sonra C. olitorius ekstraktı ile 48 saat süre muamele edildikten sonra kaspaz-3 ve Ki-67'nin dağıtımı için immünofloresan boyama yöntemiyle boyandı. Objectives: Mesenchymal stem cells are self-renewing stem cells. The human foreskin has potential to be used as a source of stem cells. The aim of the study was to obtain spheroid formation of human foreskin cells (hnFSSCs) isolated from newborn human foreskin tissue. In addition, the apoptotic and proliferative effects of a traditional plant, Corchorus olitorius L. (C. olitorius), on hnFSSC spheroids were investigated. Materials and Methods: After a routine circumcision procedure the cells were isolated and cultured in suitable medium. The plant leaves was extracted with ethanol and their composition was analyzed by liquid chromatography coupled with mass spectrometry (LC-MS/MS). The foreskin stem cells were characterized immunocytochemically by CD45, CD34, and CD90 antibodies. hnFSSC spheroids were formed using the hanging drop technique. Immunofluorescence staining was used on the obtained spheroids to determine the distribution of caspase-3 and Ki-67 after being treated with C. olitorius extract for 48 h. Results: Immunostaining analysis showed that hnFSSCs were positive for CD45 and CD34 and negative for CD90. According to LC-MS/MS C. olitorius was rich in flavanols and hydrocinnamic acid derivatives. Although the spheroids obtained were loose and floating, the cells interacted with each other. Caspase-3 activity was higher in the control group than in the extract-treated group and Ki-67 was higher in the extract-treated group than in the control group, suggesting that the plant might have the capacity to increase stem cell proliferation due to its rich polyphenolic content. Conclusion: The results suggest that hnFSSCs and spheroids might be used in stem cell generation, tissue repair and renewal as human foreskin tissue has potential to be used as a stem cell source. C. olitorius also increased proliferation of hnFSSCs, showing that polyphenols might increase proliferation of stem cells.

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Objective: To examine the relationship between physical activity level and healthy lifestyle behaviours of white-collar workers. Materials and Methods: The International Physical Activity Questionnaire Short Form (IPAQ-SF) was used to determine the level of physical activity, and the Healthy Lifestyle Behaviours Scale-II (HLBS-II) was used to evaluate the lifestyle behaviours. The researchers recorded anthropometric measurements of the participants via face-to-face method and questionnaires were implemented via online forms. Statistical analysis of the study was performed with the IBM SPSS Statistics version 20.0 program. Descriptive analysis was done, and all the data were presented as means and (±) standard deviation (SD). Statistical significance was set at p<0.05. Results: In total, 229 (mean age: 35.75, 158 female) white-collar workers participated in the study. 90.4% of the participants had either undergraduate or postgraduate degrees. 55.5% of the participants were found to be inactive as stated by the IPAQ-SF scores. According to HLBS-II results, active participants had significantly higher exercise, nutrition, and stress management sub-scale scores (p<0.05). There was no significant difference between self-actualization, interpersonal relationship, and health responsibility scores of active and inactive participants (p>0.05). Overall, active participants with normal BMI had significantly higher HLBS-II scores than overweight and obese participants (p<0.05). Conclusions: Our study shows a positive relationship between physical activity levels and healthy lifestyle behaviours in office work. In order to increase the quality of life of white-collar workers, changing their behaviours such as physical inactivity and unhealthy diet through education intervention on healthy lifestyle holds a vital place.

Colchicum pusillum Sieber is a plant from Colchicaceae family that particularly rich in tropolonic alkaloids, colchicine. The aim of the study is to determine the apoptosis induction effect of Colchicum pusillum extract in Colo-741, metastatic colon cancer cells and if so, to determine by which apoptotic pathway. Colchicum pusillum was collected and extracted with ethanol. Apoptotic activities of Colchicum pusillum were investigated by immunocytochemistry using antibodies directed against to caspase-3, cytochrome-c and FasLigand (FasL) in Colo-741 cells. Immunocytochemical staining results stated that Colchicum pusillum extract at 20 µg/mL dose, increased caspase-3 and FasL activity significantly but was not able to activate cytochrome-c activity. The results suggested that Colchicum pusillum extract induced apoptosis in metastatic colon cancer cells via extrinsic apoptotic pathway.

Bu derleme tarzı yazı Google Akademik, PubMed ve ScienceDirect vb. bilimsel veri tabanlarına 'postbiyotik', 'postbiyotik ve sağlık' anahtar kelimelerinin girilmesi üzerine elde edilen güncel literatürden yola çıkarak postbiyotiklerin sağlık üzerindeki potansiyel etkilerinin değerlendirilmesini hedeflemiştir. Canlı mikroorganizmalar tarafından üretilen postbiyotikler, cansız mikrobiyal hücreler, mikrobiyal fraksiyonlar veya hücre lizatları olarak ifade edilmektedir. Aynı zamanda postbiyotik olarak ifade edilen paraprobiyotikler ve fermente edilmiş bebek formulalarının, konakçı sağlığı üzerinde birçok fizyolojik faydası bulunmaktadır. Çocuklarda, yetişkinlerde ve yaşlılarda anti-inflamatuvar, antimikrobiyal, immünomodülatör, anti-obezojenik, anti-kanser, antioksidan, antihipertansif, hipokolesterolemik gibi sağlığı olumlu yönde etkileyen potansiyel etkiler göstermektedir. Ayrıca postbiyotiklerin diğer mikroorganizmalara (probiyotikler, prebiyotikler, sinbiyotikler) göre ek faydalar sağlayabileceği düşünülmektedir. Postbiyotiklerin bu etkilerini lokal olarak barsak epiteli üzerinden, sistematik olarak organ ve dokular üzerinden gösterdikleri bilinmektedir. Postbiyotiklerin sağlık ile ilişkisini gösteren hayvan ve insan çalışmaları mevcuttur. Postbiyotiklerin etkilerinin detaylı incelendiği (ideal doz, kronik hastalık ilişkisi, olası yan etkileri vb.) in vitro ve in vivo çalışmalara ihtiyaç vardır.