Steroid hormonal bioactivities, culprit natural and synthetic hormones and other emerging contaminants in waste water measured using bioassays and UPLC-tQ-MS

Houtman, Corine J.; Broek, Rob ten; Brouwer, Abraham

doi:10.1016/j.scitotenv.2018.02.273

Cited by 41 publications

(22 citation statements)

References 48 publications

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“…Up to 99.7% removal of estrogenic activity was observed, which fits with previous studies where between 80 and >99% removal was reported (e.g. Jugan et al., 2009 ; Jalova et al., 2013 ; Escher et al., 2014 ; Hamilton et al., 2016 ; Houtman et al., 2018 ; Nivala et al., 2018 ). Removal of GR activity could only be calculated for WWTP T1, with most samples either cytotoxic or inactive in GR GeneBLAzer.…”

Section: Resultssupporting

confidence: 91%

“…Between 67 and 69% removal of GR activity was observed. Poor to moderate removal of GR activity has previously been reported, with between -7 and 66% removal efficacy observed ( Bain et al., 2014 ; Roberts et al., 2015 ; Houtman et al., 2018 ). Oxidative stress response removal varied between WWTPs and over time, with between 10 and 90% removal efficacy observed (average of 60%) in all plants except P1 and P2.…”

Section: Resultsmentioning

confidence: 87%

“…While in vitro bioassays are recommended for water quality monitoring ( Brack et al., 2019 ), they have also been applied to evaluate treatment efficacy by wastewater, advanced water and drinking water treatment processes (e.g. Escher et al., 2011 ; Escher et al., 2014 ; Conley et al., 2017 ; Houtman et al., 2018 ). Surface waters receiving wastewater effluent have previously been shown to induce effects in both in vitro and in vivo assays (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Wastewater treatment efficacy evaluated with in vitro bioassays

et al. 2020

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Bioassays show promise as a complementary approach to chemical analysis to assess the efficacy of wastewater treatment processes as they can detect the mixture effects of all bioactive chemicals in a sample. We investigated the treatment efficacy of ten Australian wastewater treatment plants (WWTPs) covering 42% of the national population over seven consecutive days. Solid-phase extracts of influent and effluent were subjected to an in vitro test battery with six bioassays covering nine endpoints that captured the major modes of action detected in receiving surface waters. WWTP influents and effluents were compared on the basis of population- and flow-normalised effect loads, which provided insights into the biological effects exhibited by the mixture of chemicals before and after treatment. Effect removal efficacy varied between effect endpoints and depended on the treatment process. An ozonation treatment step had the best treatment efficacy, while WWTPs with only primary treatment resulted in poor removal of effects. Effect removal was generally better for estrogenic effects and the peroxisome proliferator-activated receptor than for inhibition of photosynthesis, which is consistent with the persistence of herbicides causing this effect. Cytotoxicity and oxidative stress response provided a sum parameter of all bioactive chemicals including transformation products and removal was poorer than for specific endpoints except for photosynthesis inhibition. Although more than 500 chemicals were analysed, the detected chemicals explained typically less than 10% of the measured biological effect, apart from algal toxicity, where the majority of the effect could be explained by one dominant herbicide, diuron. Overall, the current study demonstrated the utility of applying bioassays alongside chemical analysis to evaluate loads of chemical pollution reaching WWTPs and treatment efficacy.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

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Wastewater treatment efficacy evaluated with in vitro bioassays

et al. 2020

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“…Samples were extracted using Oasis HLB solid phase extraction (SPE) cartridges in portions of 1 L (surface water and control samples) or 0.4 L (WWTP effluent) per cartridge. Compounds were eluted with 1:1 methanol (MeOH) and ethylacetate as described in (Houtman et al, 2018). For each sample, three extracts were prepared in this way.…”

Section: Extractionmentioning

confidence: 99%

High resolution effect-directed analysis of steroid hormone (ant)agonists in surface and wastewater quality monitoring

Houtman

Broek

Oorschot

et al. 2020

Environmental Toxicology and Pharmacology

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“…The presence of synthetic hormones in water is associated with the use of contraception, hormonal drugs, and additives to animal fodder. As conventional wastewater treatment plants are unable to remove these substances completely, municipal wastewater and sewage from pharmaceutical plants are the main sources of hormones in the aquatic environment .…”

Section: Introductionmentioning

confidence: 99%

Molecularly imprinted polymer film grafted from porous silica for efficient enrichment of steroid hormones in water samples

Szczukocki

Krawczyk

Juszczak

et al. 2019

J of Separation Science

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Steroid hormones as endocrine disrupting compounds can interfere with the functioning of hormonal systems of organisms and thus affect the health and reproduction of humans and wildlife. Unfortunately, these types of harmful endocrine disrupting compounds have been found in a variety of environmental samples at very low concentrations. Therefore, a simple, fast, and efficient method for enrichment of water samples is needed. A molecularly imprinted solid‐phase extraction combined with high performance liquid chromatography coupled with diode array detection was developed for the determination of six steroid hormones, such as estrone, 17‐β‐estradiol, estriol, 17‐α‐ethinylestradiol, progesterone, and testosterone in water samples. The recoveries obtained in the proposed method were in the range of 78.7–101.3%. Matrix effect below 20% suggests that the quantitative and qualitative results of the analysis were not significantly affected by the matrix. The results show that molecularly imprinted polymers based on spherical silica gel had the potential to be a highly innovative and selective sorbent. The proposed method was proved to be applicable for molecularly imprinted solid‐phase extraction in selective and reliable extraction and enrichment of steroid hormones in environmental water samples.

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Steroid hormonal bioactivities, culprit natural and synthetic hormones and other emerging contaminants in waste water measured using bioassays and UPLC-tQ-MS

Cited by 41 publications

References 48 publications

Wastewater treatment efficacy evaluated with in vitro bioassays

Wastewater treatment efficacy evaluated with in vitro bioassays

High resolution effect-directed analysis of steroid hormone (ant)agonists in surface and wastewater quality monitoring

Molecularly imprinted polymer film grafted from porous silica for efficient enrichment of steroid hormones in water samples

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