High resolution effect-directed analysis of steroid hormone (ant)agonists in surface and wastewater quality monitoring

Houtman, Corine J.; Broek, Rob ten; Oorschot, Yvonne van; Kloes, D.; Oost, Ron van der; Rosielle, Martine; Lamoree, M.H.

doi:10.1016/j.etap.2020.103460

Cited by 26 publications

(14 citation statements)

References 55 publications

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“…In a further optimisation step of the platform for even higher resolution, the fractions were directly split after the LC separation, with an aliquot going to mass spectrometry analysis and the other collected in 384-well plates that could be used directly for the bioassay (Houtman et al, 2020). No concentrationresponse curve could be run but almost 300 fractions per sample provided something like a 'bioassay chromatogram' (Figure 13.7).…”

Section: Effect-directed Analysismentioning

confidence: 99%

Bioanalytical Tools in Water Quality Assessment

Neale¹,

Leusch²,

Escher³

2021

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The first edition of Bioanalytical Tools in Water Quality Assessment was released in 2012. The field has exploded since and the second edition updates and reviews the application of bioanalytical tools for water quality assessment including surveillance monitoring. The book focuses on applications to water quality assessment ranging from wastewater to drinking water, including recycled water, as well as treatment processes and advanced water treatment. Emerging applications for other environmental matrices are also included. Bioanalytical Tools in Water Quality Assessment, Second Edition, not only demonstrates applications but also fills in the background knowledge in toxicology/ecotoxicology needed to appreciate these applications. Each chapter summarises fundamental material in a targeted way so that information can be applied to better understand the use of bioanalytical tools in water quality assessment. The book can be used by lecturers teaching academic and professional courses and also by risk assessors, regulators, experts, consultants, researchers and managers working in the water sector. It can also be a reference manual for environmental engineers, analytical chemists and toxicologists. ISBN: 9781789061970 (Paperback) ISBN: 9781789061987 (eBook)

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Section: Effect-directed Analysismentioning

confidence: 99%

Bioanalytical Tools in Water Quality Assessment

Neale¹,

Leusch²,

Escher³

2021

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Section: Resultsmentioning

confidence: 99%

“…The advantage of this approach is an enhancement in sample throughput. 48 On the other hand, this approach affects chromatographic peak shapes and causes retention time shifts between the MS and bioassay chromatograms (Figure S9) that require correction. MS/MS measurement, in positive and negative ion modes, give comparable results to those obtained with multiple technical replicates.…”

Section: Identification Of Features In Bioactivementioning

confidence: 99%

High-Performance Data Processing Workflow Incorporating Effect-Directed Analysis for Feature Prioritization in Suspect and Nontarget Screening

Jonkers

Meijer

Vlaanderen

et al. 2022

Environ. Sci. Technol.

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Effect-directed analysis (EDA) aims at the detection of bioactive chemicals of emerging concern (CECs) by combining toxicity testing and high-resolution mass spectrometry (HRMS). However, consolidation of toxicological and chemical analysis techniques to identify bioactive CECs remains challenging and laborious. In this study, we incorporate state-of-the-art identification approaches in EDA and propose a robust workflow for the high-throughput screening of CECs in environmental and human samples. Three different sample types were extracted and chemically analyzed using a single high-performance liquid chromatography HRMS method. Chemical features were annotated by suspect screening with several reference databases. Annotation quality was assessed using an automated scoring system. In parallel, the extracts were fractionated into 80 micro-fractions each covering a couple of seconds from the chromatogram run and tested for bioactivity in two bioassays. The EDA workflow prioritized and identified chemical features related to bioactive fractions with varying levels of confidence. Confidence levels were improved with the in silico software tools MetFrag and the retention time indices platform. The toxicological and chemical data quality was comparable between the use of single and multiple technical replicates. The proposed workflow incorporating EDA for feature prioritization in suspect and nontarget screening paves the way for the routine identification of CECs in a high-throughput manner.

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“…Globally, many researchers paid special attention to emerging risks associated with natural and synthetic steroid hormones related to their endocrine-disrupting activities observed in receiving surface waters [47,48]. In a related joint South African and Canadian study, the estrogenicity and anti-estrogenicity of EDCs in WWTPs' influents and effluents were assessed using recombinant yeast estrogen receptor binding assays [49].…”

Section: Risk Assessment and Impacts Of Waterborne Cecs On The Environment And Human Healthmentioning

confidence: 99%

Contaminants of Emerging Concern in African Wastewater Effluents: Occurrence, Impact and Removal Technologies

2021

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Worldwide, the pollution of water bodies by contaminants of emerging concern (CECs) such as pharmaceuticals, endocrine disrupting compounds, flame retardants including brominated flame retardants (BFRs) and perfluorochemicals (PFCs), micro plastics, nanomaterials, and algal toxins, to name just a few, is creating a new set of challenges to the conventional wastewater treatment facilities, which demonstrate inefficiency in removing/degrading many CECs. As a consequence, environmentalists started to detect the presence of some of those contaminants at alarming levels in certain countries, with possible negative effects on aquatic species and often increased potential for human health risks through the exposure to the contaminated waters, or the reuse of treated wastewater in agriculture and household use. Such issues are more accentuated in the African continent due to various socio-economic problems giving rise to poor sanitation systems and serious shortages in wastewater treatment plants in many regions, making it difficult to tackle the problem of conventional pollutants, let alone to deal with the more challenging CECs. Thus, in order to effectively deal with this emerging environmental threat, African researchers are working to develop and optimize sound sampling and analytical procedures, risk assessment models, and efficient remediation technologies. In this review, related recent research efforts conducted in African universities and research institutions will be presented and discussed with respect to the occurrence and assessment of CECs in African wastewater effluents, the potential risks to aquatic ecosystems and humans, the tailored remediation techniques, along with some knowledge gaps and new research directions.

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High resolution effect-directed analysis of steroid hormone (ant)agonists in surface and wastewater quality monitoring

Cited by 26 publications

References 55 publications

Bioanalytical Tools in Water Quality Assessment

Bioanalytical Tools in Water Quality Assessment

High-Performance Data Processing Workflow Incorporating Effect-Directed Analysis for Feature Prioritization in Suspect and Nontarget Screening

Contaminants of Emerging Concern in African Wastewater Effluents: Occurrence, Impact and Removal Technologies

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