Bioanalytical Tools in Water Quality Assessment

Neale, Peta A.; Leusch, Frederic; Escher, Beate I.

doi:10.2166/9781789061987

Cited by 62 publications

(108 citation statements)

References 231 publications

(491 reference statements)

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“…5,6 In vitro cell-based reporter gene bioassays are highly sensitive, target specific modes of actions, allow high-throughput applications, and can be used for the effect characterization of the chemical mixture present in an environmental sample. 7 However, the bioavailability of chemicals in the bioassay system can be influenced by various processes, such as binding to medium constituents (lipids and proteins), 8,9 sorption to the well plate, 10,11 and, in the case of biota samples, binding to co-extracted lipids 2,12 that form droplets on the medium surface. Co-dosed lipids add an additional partitioning phase to the bioassay system and can thus reduce the bioavailability of chemicals.…”

Section: ■ Introductionmentioning

confidence: 99%

Influence of Co-Dosed Lipids from Biota Extracts on the Availability of Chemicals in In Vitro Cell-Based Bioassays

Reiter

Jahnke

König

et al. 2020

Environ. Sci. Technol.

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Extraction of chemicals from biota leads to co-extraction of lipids. When dosing such extracts into in vitro bioassays, co-dosed lipids act as an additional phase that can reduce the bioavailability of the chemicals and the apparent sensitivity of the assay. Equilibrium partitioning between medium, cells, and co-dosed lipids was described with an existing equilibrium partitioning model for cell-based bioassays extended by an additional lipid phase. We experimentally investigated the influence of co-dosed lipids on the effects elicited by four test chemicals of different hydrophobicity in two bioassays, indicative of the aryl hydrocarbon receptor and oxidative stress response (AREc32). The partitioning model explained the effect of the test chemicals in the presence of spiked triolein within a factor of 0.33−5.83 between the measured and predicted effect concentration (EC). We applied the model to marine mammal blubber extracted with silicone. Extracts dosed in the AREc32 bioassay showed a linear increase of apparent EC with increasing lipid fraction. The partitioning model was used to interpret the role of the co-extracted lipid. A quantitative lipid correction of bioassay results in the presence of co-dosed lipids was possible for known compounds and defined mixtures, while we could only estimate a range for mixtures of unknown chemicals.

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Section: ■ Introductionmentioning

confidence: 99%

Influence of Co-Dosed Lipids from Biota Extracts on the Availability of Chemicals in In Vitro Cell-Based Bioassays

Reiter

Jahnke

König

et al. 2020

Environ. Sci. Technol.

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“…Effect-based tests that covered reactive, non-specific, and specific modes of actions were applied (Escher et al 2021).…”

Section: Effect-based In Vitro Methodsmentioning

confidence: 99%

“…Effect-based tests that covered reactive, non-specific, and specific modes of actions were applied (Escher et al 2021 ). The methods assessed activation of AhR, androgenicity (AR), estrogenicity (ER), oxidative stress (Nrf2), and genotoxicity (micronucleus test) (Table 2 ).…”

Section: Methodsmentioning

confidence: 99%

“…Thus, alternative methods for toxicity testing would be useful (Montazeri et al 2013 ). Effect-based methods, often based on cultured mammalian cells that have been modified to respond to key molecular events early in toxicity pathways, have proven to be valuable to evaluate highly complex mixtures (Escher et al 2021 ; Rosenmai et al 2017 ; Selin et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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An in vitro-based hazard assessment of liquid smoke food flavourings

et al. 2021

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Liquid smoke products are widely used as a food additive to create a desired smoke flavour. These products may contain hazardous chemicals generated during the wood-burning process. However, the toxic effects of these types of hazardous chemicals constituting in the commercially available products are largely unknown. Therefore, a test battery of cell-based in vitro methods, covering different modes of actions of high relevance to human health, was applied to study liquid smoke products. Ten liquid smoke flavourings were tested as non-extracted and extracted. To assess the potential drivers of toxicity, we used two different solvents. The battery of in vitro methods covered estrogenicity, androgenicity, oxidative stress, aryl hydrocarbon receptor activity and genotoxicity. The non-extracted samples were tested at concentrations 0.002 to 1 μL liquid smoke flavouring/mL culture medium, while extracted samples were tested from 0.003 to 200 μL/mL. Genotoxicity was observed for nearly all non-extracted and all hexane-extracted samples, in which the former had higher potency. No genotoxicity was observed for ethyl acetate-extracted samples. Oxidative stress was activated by almost all extracted and non-extracted samples, while approximately half of the samples had aryl hydrocarbon receptor and estrogen receptor activities. This study used effect-based methods to evaluate the complex mixtures of liquid smoke flavourings. The increased bioactivities seen upon extractions indicate that non-polar chemicals are driving the genotoxicity, while polar substances are increasing oxidative stress and cytotoxic responses. The differences in responses indicate that non-extracted products contain chemicals that are able to antagonize toxic effects, and upon extraction, the protective substances are lost.

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“…Moreover, chemicals exist as complex mixtures in most impacted surface waters, posing risks that may differ from those predicted on the basis of their individual components, due to interactive effects. As a result, there is increasing emphasis on the development and application of tools that directly measure biological impacts of environmental contaminants on a broad spectrum of end points, ranging from molecular (e.g., receptor binding and altered gene transcription) to apical (e.g., decreased reproduction, morbidity, and behavioral). , Metabolomics, which measures the full complement of detectable endogenous metabolites (i.e., the metabolome) in a tissue or biofluid, is one of the most promising of these bioeffects tools, and as such, it is increasingly being employed in environmental toxicology . This is due in part to the large number of metabolites that can be assessed (hundreds to thousands) and, as a result, to the broad range of toxic responses that can be captured in an untargeted fashion.…”

Section: Introductionmentioning

confidence: 99%

Untargeted Lipidomics for Determining Cellular and Subcellular Responses in Zebrafish (Danio rerio) Liver Cells Following Exposure to Complex Mixtures in U.S. Streams

Zhen

Teng

Mosley

et al. 2021

Environ. Sci. Technol.

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Surface waters often contain a variety of chemical contaminants potentially capable of producing adverse outcomes in both humans and wildlife due to impacts from industrial, urban, and agricultural activity. Here, we report the results of a zebrafish liver (ZFL) cell-based lipidomics approach to assess the potential ecotoxicological effects of complex contaminant mixtures using water collected from eight impacted streams across the United States mainland and Puerto Rico. We initially characterized the ZFL lipidome using high resolution mass spectrometry, resulting in the annotation of 508 lipid species covering 27 classes. We then identified lipid changes induced by all streamwater samples (nonspecific stress indicators) as well as those unique to water samples taken from specific streams. Subcellular impacts were classified based on organelle-specific lipid changes, including increased lipid saturation (endoplasmic reticulum stress), elevated bis(monoacylglycero)phosphate (lysosomal overload), decreased ubiquinone (mitochondrial dysfunction), and elevated ether lipids (peroxisomal stress). Finally, we demonstrate how these results can uniquely inform environmental monitoring and risk assessments of surface waters.

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Bioanalytical Tools in Water Quality Assessment

Cited by 62 publications

References 231 publications

Influence of Co-Dosed Lipids from Biota Extracts on the Availability of Chemicals in In Vitro Cell-Based Bioassays

Influence of Co-Dosed Lipids from Biota Extracts on the Availability of Chemicals in In Vitro Cell-Based Bioassays

An in vitro-based hazard assessment of liquid smoke food flavourings

Untargeted Lipidomics for Determining Cellular and Subcellular Responses in Zebrafish (Danio rerio) Liver Cells Following Exposure to Complex Mixtures in U.S. Streams

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