Analysis of endocrine disruption effect of Roundup ® in adrenal gland of male rats

Abstract

The effect of Roundup® on adrenal gland steroidogenesis and signaling pathway associated with steroid production was investigated. Doses of 10, 50, 100 and 250 mg/kg bw/d Roundup® were administered for two weeks to adult male rats. The 10 mg/kg bw/d dose which reduced circulatory corticosterone levels, but did not change food consumption and body weight, was selected for further study. The expression of cholesterol receptor (low density lipoprotein receptor), de novo cholesterol synthesis enzyme (3-hydroxy-3-methylglutaryl-coenzyme A synthase), hormone-sensitive lipase, steroidogenic acute regulatory protein (StAR) mRNA and phosphorylated form was decreased. Adrenocorticotropic hormone receptor (ACTH), melanocortin-2 receptor, expression was not changed but circulatory ACTH levels and adrenal cortex protein kinase A (PKA) activity were reduced. Surprisingly, exogenous ACTH treatment rescued steroidogenesis in Roundup®-treated animals. Apoptosis was evident at 250 mg/kg bw/d, but not at 10 mg/kg bw/d dose. These results suggest that Roundup® may be inhibitory to hypothalamic–pituitary axis leading to reduction in cyclic adenosine monophosphate (cAMP)/PKA pathway, StAR phosphorylation and corticosterone synthesis in the adrenal tissue.

Death as a Drug Side Effect in FAERS: Is Glyphosate Contamination a Factor?

“…This condition manifests as fatigue, anorexia, sweating, anxiety, shaking, nausea, heart palpitations and weight loss." ( [29], p. 1084). Based on this list, we created a table showing how these symptoms distributed among KIDNEYFAILURE1 and KIDNEYFAILURE2.…”

“…Guided by a recent paper studying the effects of small doses of Roundup ® on rats [29], we decided to investigate how our two renal failure subsets distributed with respect to symptoms related to adrenal insufficiency. Pandey et al [29] showed that 10 mg/kg doses of Roundup ® administered over a two-week period to male rats resulted in a marked drop in steroidogenesis in the adrenal glands.…”

“…Pandey et al [29] showed that 10 mg/kg doses of Roundup ® administered over a two-week period to male rats resulted in a marked drop in steroidogenesis in the adrenal glands. They showed that this was related to a reduced supply of adrenocorticotrophic hormone (ACTH) from the pituitary gland, which caused reduced expression of steroidogenic acute regulatory protein (StAR) in the adrenal glands.…”

“…TRASYLOL is remarkably over- Table 9. Counts and scores for the KIDNEYFAILURE1 set (kidney failure with associated oedema, skin, or gut issues), compared to KIDNEYFAILURE2 (the remainder of the KIDNEY FAILURE cases), for various symptoms linked to adrenal insufficiency, as defined in [29]. The scores are computed as previously defined.…”

“…By subselecting for oedema along with conditions relating to the skin and gut, we were able to obtain a subpopulation with kidney failure that also exhibited characteristic symptoms of adrenal insufficiency. Adrenal insufficiency has recently been linked to low-dose glyphosate exposure [29].…”