Integrated metabolomics and transcriptomics reveal glyphosate based‐herbicide induced reproductive toxicity through disturbing energy and nucleotide metabolism in mice testes

Qı, Lei; Li, Yupeng; Dong, Yanmei; Ma, Shu-Li; Li, Gang

doi:10.1002/tox.23808

Cited by 4 publications

(2 citation statements)

References 49 publications

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“…Toxicity assessment, through metabolomics analysis of urine or blood samples collected to detect the physiological changes and toxic damage caused by the substance [164][165][166]. The main advantage of using metabolomics in toxicology is that alterations in metabolism are terminal events to genetic, transcriptomic, and proteomic levels.…”

Section: Toxicity Assessmentmentioning

confidence: 99%

“…Thus, metabolomics facilitates the understanding of direct cellular phenotypes that are induced by toxic insults. In addition, since changes in extracellular metabolites mirror the intracellular metabolic scenario, metabolomics analysis of biological fluids obtained through non-invasive or minimally invasive techniques suffices for the evaluation of target organ toxicities [166][167][168]. Another advantage of metabolomics is that information on a large number of metabolites could be obtained through a single measurement, an oftentimes-basic requirement for rapidly understanding the level of drug toxicity.…”

Section: Toxicity Assessmentmentioning

confidence: 99%

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Review on analytical technologies and applications in metabolomics

MENG,

LIU,

et al. 2024

BIOCELL

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Over the past decade, the swift advancement of metabolomics can be credited to significant progress in technologies such as mass spectrometry, nuclear magnetic resonance, and multivariate statistics. Currently, metabolomics garners widespread application across diverse fields including drug research and development, early disease detection, toxicology, food and nutrition science, biology, prescription, and chinmedomics, among others.Metabolomics serves as an effective characterization technique, offering insights into physiological process alterations in vivo. These changes may result from various exogenous factors like environmental conditions, stress, medications, as well as endogenous elements including genetic and protein-based influences. The potential scientific outcomes gleaned from these insights have catalyzed the formulation of innovative methods, poised to further broaden the scope of this domain. Today, metabolomics has evolved into a valuable and widely accepted instrument in the life sciences. However, comprehensive reviews focusing on the sample preparation and analytical methodologies employed in metabolomics within the life sciences are surprisingly scant. This review aims to fill that gap, providing an overview of current trends and recent advancements in metabolomics. Particular emphasis is placed on sample preparation, sophisticated analytical techniques, and their applications in life science research.

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Section: Toxicity Assessmentmentioning

confidence: 99%

Section: Toxicity Assessmentmentioning

confidence: 99%

Review on analytical technologies and applications in metabolomics

MENG,

LIU,

et al. 2024

BIOCELL

View full text Add to dashboard Cite

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A mixture of glyphosate and 2,4‐D herbicides enhances the deleterious reproductive outcomes induced by Western diet in obese male mice

Valente,

de Matos Manoel,

Reis

et al. 2023

Environmental Toxicology

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The consumption of Western diet (WD) – enriched in fats and sugars – is associated with overweight, obesity and male reproductive disorders. In addition to WD intake, crops and dairy products display residues of herbicides, including glyphosate and 2,4‐D that are widely applied worldwide. The concomitant exposure to WD and herbicides – mimicking contemporary scenarios – is not fully investigated. Thus, we evaluated the effects of glyphosate and 2,4‐D, alone or in mixture, on WD‐induced alterations in the male genital system. Male C57BL6J mice were submitted to WD (chow containing 20% lard, 0.2% cholesterol, 20% sucrose, and high sugar solution with 23.1 and 18.9 g/L of D‐fructose and D‐glucose) for 6 months. Concomitantly to WD, the animals received glyphosate (0.05, 5, or 50 mg/kg/day), 2,4‐D (0.02, 2 or 20 mg/kg/day) or their mixture (0, 05 + 0.02, 5 + 2, or 50 + 20 mg/kg/day) by intragastrical administration (5×/week). Doses were based on Acceptable Daily Intake (ADIs) or No Observed Adverse Effect Level (NOAEL) values. Herbicide exposure did not alter the WD‐induced obesity, hypercholesterolemia and hyperglycemia. WD induced sperm cell abnormalities, reduced the number, volume and area of Leydig cells, enhanced the frequency of epididymal abnormalities, decreased the proliferation in both germinal and epididymal epithelia, and reduced the number of androgen receptor (AR) positive epididymal cells. Remarkably, the herbicide mixtures promoted such WD‐induced effects: increased the frequency of sperm cell and epididymal abnormalities (absence of sperm, cytoplasmic vacuoles, and clear cell hypertrophy) (5 + 2 and 50 + 20 doses); decreased Leydig cell nuclei volume and area (5 + 2 and 50 + 20 doses), reduced epididymal cell proliferation (all mixtures), and AR expression (50 + 20 dose). In addition, herbicide mixtures reduced serum testosterone levels (5 + 2 and 50 + 20 doses). Our findings indicate that the mixture of glyphosate and 2,4‐D herbicides, mimicking environmentally relevant scenarios, promotes WD‐induced changes in the male genital system.

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