RNA aptamer-based electrochemical aptasensor for C-reactive protein detection using functionalized silica microspheres as immunoprobes

Wang, Junchun; Guo, Jinjin; Zhang, Junjun; Zhang, Wenjuan; Zhang, Yuzhong

doi:10.1016/j.bios.2017.04.014

Cited by 101 publications

(36 citation statements)

References 37 publications

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“…Most of the works presented use the ferrocyanide redox pair for detection [26,[30][31][32][33]35], however they have not been evaluated for reuse. The immunosensor reported in this work has simple methodology, high specificity and selectivity to detect PCR in real serum sample.…”

Section: Resultsmentioning

confidence: 99%

Reusable Immunosensor for Detection of C‐reactive Protein in Human Serum

et al. 2020

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This work describes the preparation of a reusable electrochemical immunosensor for detection of C-reactive protein (CRP), based in modified carbon electrode with reduced graphene oxide and polytyramine as strategy for anchoring the anti-CRP probe. This immunosensor was able to distinguish different concentrations of CRP target in human serum, by differential pulse voltammetry, showing a linear response from 1.09 to 100 μg L À 1. The performance of the immunosensor allowed its application in four consecutive assays, without activity loss. Analyses by atomic force microscopy, scanning electron microscopy and electrochemical impedance spectroscopy corroborate with voltammetric analysis. The immunosensor showed selectivity, stability and reusability, being a promising platform for point-of-care quantification of CRP.

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Section: Resultsmentioning

confidence: 99%

Reusable Immunosensor for Detection of C‐reactive Protein in Human Serum

et al. 2020

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“…Another study reported the development of aptamer-conjugated gold-coated magnetic NPs for intelligent detection of leukemia cancer cells. 37 In addition to cancer detection, aptasensors have been used to trace microRNAs, 38 C-reactive protein, 39 and lysozyme. 40…”

Section: Diagnosis Formentioning

confidence: 99%

<p>Aptamers as Versatile Ligands for Biomedical and Pharmaceutical Applications</p>

Guan¹,

Zhang²

2020

IJN

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Aptamers are a class of targeting ligands that bind exclusively to biomarkers of interest. Aptamers have been identified as candidates for the construction of various smart systems for therapy, diagnosis, bioimaging, and drug delivery due to their high target affinity and specificity. Aptamers are accounted as chemical antibodies that can be readily linked to drugs, sensors, signal enhancers, or nanocarriers for functionalization. Use of aptamer-guided medications, especially nanomedicines, has resulted in encouraging outcomes compared to those use of aptamer-free counterparts. This article reviews recent advances in the use of aptamers as targeting ligands for various biomedical and pharmaceutical purposes. Special interests focus on aptamer-based theranostics, biosensing, bioimaging, drug potentiation, and targeted drug delivery.

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“…The SWV was employed to record the sensing signals generated upon the interaction between a sandwich structure of aptamer-CRP-immuno probe. The developed aptasensor exhibited a large linear range (0.005 ng/mL to 125 ng/mL) for the detection of CRP with excellent LOD at 0.0017 ng/mL [64]. Furthermore, Tertis et al have recently described a label-free electrochemical aptasensor for the sensitive recognition of IL-6 in human serum.…”

Section: Aptasensors For Cvd Biomarkersmentioning

confidence: 99%

Application of Electrochemical Aptasensors toward Clinical Diagnostics, Food, and Environmental Monitoring: Review

Mohamed

Mohan

et al. 2019

Sensors

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Aptamers are synthetic bio-receptors of deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) origin selected by the systematic evolution of ligands (SELEX) process that bind a broad range of target analytes with high affinity and specificity. So far, electrochemical biosensors have come up as a simple and sensitive method to utilize aptamers as a bio-recognition element. Numerous aptamer based sensors have been developed for clinical diagnostics, food, and environmental monitoring and several other applications are under development. Aptasensors are capable of extending the limits of current analytical techniques in clinical diagnostics, food, and environmental sample analysis. However, the potential applications of aptamer based electrochemical biosensors are unlimited; current applications are observed in the areas of food toxins, clinical biomarkers, and pesticide detection. This review attempts to enumerate the most representative examples of research progress in aptamer based electrochemical biosensing principles that have been developed in recent years. Additionally, this account will discuss various current developments on aptamer-based sensors toward heavy metal detection, for various cardiac biomarkers, antibiotics detection, and also on how the aptamers can be deployed to couple with antibody-based assays as a hybrid sensing platform. Aptamers can be used in various applications, however, this account will focus on the recent advancements made toward food, environmental, and clinical diagnostic application. This review paper compares various electrochemical aptamer based sensor detection strategies that have been applied so far and used as a state of the art. As illustrated in the literature, aptamers have been utilized extensively for environmental, cancer biomarker, biomedical application, and antibiotic detection and thus have been extensively discussed in this article.

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RNA aptamer-based electrochemical aptasensor for C-reactive protein detection using functionalized silica microspheres as immunoprobes

Cited by 101 publications

References 37 publications

Reusable Immunosensor for Detection of C‐reactive Protein in Human Serum

Reusable Immunosensor for Detection of C‐reactive Protein in Human Serum

<p>Aptamers as Versatile Ligands for Biomedical and Pharmaceutical Applications</p>

Application of Electrochemical Aptasensors toward Clinical Diagnostics, Food, and Environmental Monitoring: Review

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