In vitro enamel erosion and abrasion-inhibiting effect of different fluoride varnishes

Alexandria, Adílis Kalina; Vieira, Thiago Isidro; Pithon, Matheus Melo; Fidalgo, Tatiana Kelly da Silva; Fonseca‐Gonçalves, Andréa; Valença, Ana Maria Gondim; Cabral, Lúcio Mendes; Maia, Lucianne Cople

doi:10.1016/j.archoralbio.2017.01.010

Cited by 40 publications

(45 citation statements)

References 28 publications

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“…After 6 h of immersion in artificial saliva (1.5 mmol/L Ca, 0.9 mmol/L P, 150 mmol/L KCl, 0.05 mg F/mL in 0.1 mol/L Tris buffer, pH 7.0, 25 mL/sample) (12,13), the layer of varnish was removed using a scalpel blade and acetone with water (1:1) (13-15), the total removal was checked microscopically (´40).…”

Section: Treatment and Erosive Cyclingmentioning

confidence: 99%

“…After 4 days of experiment, the layer of acid-resistant nail varnish was removed using acetone with water (1:1) (13)(14)(15), allowing the comparison between the unexposed and exposed areas.…”

Section: Profilometric Analysismentioning

confidence: 99%

“…The images were analyzed by Nanovea Professional 3D software (Nanovea PS50 Optical, NANOVEA Inc.) to determine tooth structure loss (TSL) and surface roughness (Sa), according to previous studies (13,17).…”

Section: Profilometric Analysismentioning

confidence: 99%

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Fluoride Varnishes against Dental Erosion Caused by Soft Drink Combined with Pediatric Liquid Medicine

et al. 2017

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The present study evaluated the effect of NaF and CPP-ACP/NaF varnishes to reduce erosion produced by soft drink (SD) combined or not with pediatric liquid medicine. Enamel specimens were pre-treated with fluoride varnish, according to the following groups: NaF varnish (Duraphat®) or CPP-ACP/NaF varnish (MI varnish TM ). Two types of erosive cycles were made: by soft drink erosion (SDE) or by pediatric liquid medicine plus soft drink erosion (PLM/SDE). Bovine enamel specimens were randomly assigned in six groups (n=10): G1=NaF + SDE; G2=CPP-ACP/NaF + SDE; G3=Distilled and deionized (DD) water + SDE; G4=NaF + PLM/SDE; G5=CPP-ACP/NaF + PLM/SDE and G6=DD water + PLM/SDE. Before treatments, the sample surface was divided in two areas (unexposed area-UA and exposed area-EA). The specimens were evaluated by 3D non-contact profilometry technique to determinate tooth structure loss (TSL) and surface roughness (Sa). Scanning electron microscopy (SEM) analysis was also performed. After SDE, G2 presented the lowest TSL values compared to G3 (p=0.008). G1 and G2 did not differ between them (p=0.203) and no groups differed among them despite Sa. Regarding TSL and Sa, G4 and G5 differed from G6 (p=0.0001), but not between them (p=1.00). Examining 3D and SEM images, the greatest differences between UA and EA were observed for G3 and G6. CPP-ACP/ NaF varnish seems to be a promising treatment to reduce enamel loss from the erosion produced by a soft drink. Both varnishes also showed capacity to reduce TSL and Sa after erosion by soft drink combined to pediatric liquid medicine.

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Section: Treatment and Erosive Cyclingmentioning

confidence: 99%

Section: Profilometric Analysismentioning

confidence: 99%

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Fluoride Varnishes against Dental Erosion Caused by Soft Drink Combined with Pediatric Liquid Medicine

et al. 2017

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“…16 The EDS analysis was performed with Kontron, a link and automatic image analyzer system, which provided the following double-way measurements: evaluation of the total area of the block and evaluation of three equidistant points (1-mm distance between pointscenter, left, and right) on each selected block. The results were represented by the mean of the measured values.…”

Section: Cross-sectional Microhardness Analysismentioning

confidence: 99%

“…16 Subsequently, a plastic mesh was used to cover the blocks in order to allow dental biofilm accumulation, and a 20% sucrose solution was applied to provide a carbohydrate challenge. Volunteers were instructed to remove the appliances from the oral cavity and to apply one drop of the sucrose solution on each enamel block 10 times/ day 17 ( Figure 1).…”

Section: In Situ Protocolmentioning

confidence: 99%

In situ effect of titanium tetrafluoride varnish on enamel demineralization

et al. 2017

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The effect of a 4% titanium tetrafluoride (TiF 4 ) varnish on enamel demineralization was evaluated. Twelve volunteers participated in this double-blind, randomized crossover study. Six enamel specimens were positioned in intraoral appliances throughout four treatment stages: 4% TiF 4 varnish (experimental varnish), 5% sodium fluoride (NaF) varnish (Duraphat ® ), placebo varnish, and negative control (deionized water). After 24 h, the varnishes were removed and plaques were allowed to accumulate. A 20% sucrose solution was dripped onto enamel blocks (10x/day). Enamel alterations were analyzed by surface microhardness (SMH), percentage of surface loss (%SML), cross-sectional microhardness (CSMH), scanning electron microscopy (SEM), and energy dispersive X-ray spectrometry (EDS). Student's paired t-test was used for SMH analysis and repeated-measures analysis of variance (ANOVA) for %SML and CSMH (∆Z) analyses (p-value=0.05). The TiF 4 varnish group had lower %SML than the placebo and control groups (p=0.044 and p=0.003, respectively), thus showing its capacity to inhibit surface demineralization. TiF 4 and NaF varnishes demonstrated a protective effect against mineral loss on the enamel subsurface. Both were statistically different from the control group when CSMH was analyzed (p=0.000). A titanium dioxide film was observed on enamel surfaces of the TiF 4 group SEM images. EDS confirmed the presence of titanium in all TiF 4 samples. The 4% TiF 4 varnish is a promising compound capable of reacting with enamel to protect it against surface and subsurface demineralization.

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Effect of TiF4 varnish on microbiological changes and caries prevention: in situ and in vivo models

et al. 2018

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In vitro enamel erosion and abrasion-inhibiting effect of different fluoride varnishes

Cited by 40 publications

References 28 publications

Fluoride Varnishes against Dental Erosion Caused by Soft Drink Combined with Pediatric Liquid Medicine

Fluoride Varnishes against Dental Erosion Caused by Soft Drink Combined with Pediatric Liquid Medicine

In situ effect of titanium tetrafluoride varnish on enamel demineralization

Effect of TiF4 varnish on microbiological changes and caries prevention: in situ and in vivo models

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