Portable and low cost fluorescence set-up for in-situ screening of Ochratoxin A

Bueno, Diana; Mishra, Rupesh K.; Hayat, Akhtar; Catanante, Gaëlle; Sharma, Vinay; Castro-Muñoz, Roberto; Marty, Jean‐Louis

doi:10.1016/j.talanta.2016.06.039

Cited by 10 publications

(6 citation statements)

References 27 publications

(33 reference statements)

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“…More recent work (Barthelmebs et al, 2011) achieved the detection limit for OTA of 1 ng/mL using ELISA aptamer assay. The detection limit for OTA detection in aptamer assay was lowered substantially down to single ng/ml using other transducing techniques such as electrochemical, optical and piezo-electrical methods (Yang et al, 2012;Bueno et al, 2016;Catanante et al, 2016;Mishra et al, 2016). Aptamers can be immobilised on the surface of gold via functional thiol group on one end (Balamurugan et al,2008), and may contain either fluorescent groups or redox labels such as methylene blue on the other end as described by (Sheng et al, 2011;Ferapontova and Gothelf, 2009) and therefore can be used in optical or electrochemical biosensors.…”

Section: Introductionmentioning

confidence: 99%

Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry

Rubaye

Nabok

Catanante

et al. 2018

Sensors and Actuators B: Chemical

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The current work is a continuation of our research targeted the development of novel optical sensing technologies for detection of mycotoxins. The method of (TIRE) was developed in the last decade as a combination of spectroscopic ellisometry and SPR and was proved to be a highly sensitive analytical tool in bio-sensing particularly attractive for detection of low molecular weight analytes, such as mycotoxins. The use of aptamers as highly specific artificial molecular receptors to ochratoxin A (OTA) in conjunction with the method Total Internal Reflection Ellipsometry (TIRE) is reported here for the first time. Our results showed a possibility of label-free optical detection of OTA down to 0.01 ppb in concentration in direct assay with specific aptamer. The kinetics of aptamer/OTA binding was studied with dynamic TIRE spectral measurements and allowed evaluating the affinity constant K D = 1.8. 10-8 Mol which is characteristic for highly specific aptamer/OTA binding.

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Section: Introductionmentioning

confidence: 99%

Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry

Rubaye

Nabok

Catanante

et al. 2018

Sensors and Actuators B: Chemical

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“…This presented MNPs-SPEs sensor offers higher sensitivity as compared with other methods in the detection of OTA, including fluorescence methods [ 33 , 34 , 35 ], enzyme immunoassay [ 30 ], fluorescence polarization (FP) analysis [ 36 ], and other different sensor methods [ 37 , 38 , 39 , 40 , 41 ]. Compared with other ultrasensitive techniques, such as fluorescence resonance energy transfer (FRET) aptasensor [ 42 ] and impedimetric aptasensor [ 43 ], our MNPs-SPEs sensor offers a similar detection limit, but in a shorter detection time.…”

Section: Resultsmentioning

confidence: 99%

Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

Zhang

Wang

Xie

et al. 2018

Toxins

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A rapid and sensitive electrochemical biosensor based on magnetic nanoparticles and screen-printed electrodes (MNPs-SPEs sensor) was developed for the detection of ochratoxin A (OTA) in cereal and feed samples. Different types of magnetic nanoparticles-based ELISA (MNPs-ELISA) were optimized, and the signal detection, as well as sensitivity, was enhanced by the combined use of screen-printed electrodes (SPEs). Under the optimized conditions, the calibration curve of the MNPs-SPEs sensor was y = 0.3372x + 0.8324 (R2 = 0.9805). The linear range of detection and the detection limit were 0.01–0.82 ng/mL and 0.007 ng/mL, respectively. In addition, 50% inhibition (IC50) was detectable at 0.10 ng/mL. The limit of detection (LOD) of this MNPs-SPEs sensor in cereal and feed samples was 0.28 μg/kg. The recovery rates in spiked samples were between 78.7% and 113.5%, and the relative standard deviations (RSDs) were 3.6–9.8%, with the coefficient of variation lower than 15%. Parallel analysis of commercial samples (corn, wheat, and feedstuff) showed a good correlation between MNPs-SPEs sensor and liquid chromatography tandem mass spectrometry (LC/MS-MS). This new method provides a rapid, highly sensitive, and less time-consuming method to determine levels of ochratoxin A in cereal and feedstuff samples.

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“…And aptamer-based biosensors are considered a promising molecular diagnostic approach in the future. At present, there are many methods based on aptamer for detection of OTA, such as electrochemical [ 40 , 41 , 42 ], colorimetry [ 43 ], fluorescence [ 44 , 45 ], chemiluminescence [ 46 ] and up-converting luminescence [ 47 ]. Although they have good sensitivity and specificity, they cannot be used for onsite one-step detection with the following disadvantages: a small linear range in tests, complex synthesis and immobilization process of nanoparticles or sensor.…”

Section: Introductionmentioning

confidence: 99%

A Lateral Flow Strip Based Aptasensor for Detection of Ochratoxin A in Corn Samples

et al. 2018

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Ochratoxin A (OTA) is a mycotoxin identified as a contaminant in grains and wine throughout the world, and convenient, rapid and sensitive detection methods for OTA have been a long-felt need for food safety monitoring. Herein, we presented a new competitive format based lateral flow strip fluorescent aptasensor for one-step determination of OTA in corn samples. Briefly, biotin-cDNA was immobilized on the surface of a nitrocellulose filter on the test line. Without OTA, Cy5-labeled aptamer combined with complementary strands formed a stable double helix. In the presence of OTA, however, the Cy5-aptamer/OTA complexes were generated, and therefore less free aptamer was captured in the test zone, leading to an obvious decrease in fluorescent signals on the test line. The test strip showed an excellent linear relationship in the range from 1 ng·mL−1 to 1000 ng·mL−1 with the LOD of 0.40 ng·mL−1, IC15 value of 3.46 ng·mL−1 and recoveries from 96.4% to 104.67% in spiked corn samples. Thus, the strip sensor developed in this study is an acceptable alternative for rapid detection of the OTA level in grain samples.

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Portable and low cost fluorescence set-up for in-situ screening of Ochratoxin A

Cited by 10 publications

References 27 publications

Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry

Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry

Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples

A Lateral Flow Strip Based Aptasensor for Detection of Ochratoxin A in Corn Samples

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