Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry

Rubaye, Ali Al; Nabok, Alexei; Catanante, Gaëlle; Marty, Jean‐Louis; Takács, E.C.; Székacs, András

doi:10.1016/j.snb.2018.01.220

Cited by 37 publications

(34 citation statements)

References 29 publications

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“…A similar approach was employed in the study of aflatoxin B 1 [219]. Recently, TIRE has also been coupled with SPR for human immunodeficincy virus diagnostics [220], mycotoxin recognition [221] as well as micromolar immunoglobulin determination [222]. TIRE has also been explored for applications in bioimaging with the help of AuNPs [223].…”

Section: Ellipsometric Biosensorsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

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Section: Ellipsometric Biosensorsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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“…Immobilization of monoclonal Ab against AFB 1 allowed detection of 0.01 ng/ml of the analyte by shift of refractive index. Total internal reflectance ellipsometry has been applied for detection of OTA bonded to specific aptamers immobilized on gold-coated glass slices [57]. A similar technique was exploited here for detection of AFB 1 and AFM 1 with specific aptamers immobilized on the surface of golden films [58].…”

Section: Other Sensors For Mycotoxin Determinationmentioning

confidence: 99%

Electrochemical Immuno- and Aptasensors for Mycotoxin Determination

Evtugyn

Hianik

2019

Chemosensors

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Modern analysis of food and feed is mostly focused on development of fast and reliable portable devices intended for field applications. In this review, electrochemical biosensors based on immunological reactions and aptamers are considered in the determination of mycotoxins as one of most common contaminants able to negatively affect human health. The characteristics of biosensors are considered from the point of view of general principles of bioreceptor implementation and signal transduction providing sub-nanomolar detection limits of mycotoxins. Moreover, the modern trends of bioreceptor selection and modification are discussed as well as future trends of biosensor development for mycotoxin determination are considered.

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“…Aptamers labelled with redox groups were successfully used for electrochemical detection of OTA [23]. Recently, we used similar anti-OTA aptamers, though without label, in combination with the TIRE method, and achieved the detection of OTA down to 0.01 ng/ml concentrations [24]. In the current work, we used non-labeled aptamers (from Microsynth, Switzerland) for AFT B1 having the following nucleotide sequence: with SH group at 5' (or C5) terminal and label-free terminal 3' (or C3).…”

Section: Bio-receptors For Detection Of Mycotoxinsmentioning

confidence: 99%

[INVITED] Novel optical biosensing technologies for detection of mycotoxins

Nabok

Al-Rubaye

Al-Jawdah

et al. 2019

Optics & Laser Technology

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This work reviews our recent progress in development of novel optical methods of detection of mycotoxins in direct assay with either specific antibodies or aptamers. The main method in this work was the total internal reflection ellipsometry (TIRE) combined with LSPR transducers based on gold nano-structures produced by annealing of thin gold films. The gold nano-islands produced were characterised with SEM, AFM, UV-visible absorption spectroscopy, and spectroscopic ellipsometry. The combination of TIRE and LSPR offers superior refractive index sensitivity as compared to traditional UV-vis absorption spectroscopy. The limitations of LSPR related to a short evanescent field decay length can be overcome using small-size bio-receptors, such as halfantibodies and aptamers. The achieved sensitivity of detection of mycotoxins in 0.01ppb level of concentration is sufficient for the use of this method for analysis of agriculture products, food and feed on the presence of mycotoxins. Even higher sensitivity in sub-ppt level was achieved with another optical biosensor developed recently; it is based on optical planar waveguide operating as polarization interferometer (PI). This method is promising for development of portable, highly sensitive, and simple to use biosensors suitable for point-of-need detection of mycotoxins.

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Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry

Cited by 37 publications

References 29 publications

Recent Progress in Optical Sensors for Biomedical Diagnostics

Recent Progress in Optical Sensors for Biomedical Diagnostics

Electrochemical Immuno- and Aptasensors for Mycotoxin Determination

[INVITED] Novel optical biosensing technologies for detection of mycotoxins

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