Choosing the right type of serum for different applications of human adipose tissue–derived stem cells: influence on proliferation and differentiation abilities

Koellensperger, Eva; Bollinger, Nils; Dexheimer, Verena; Gramley, Felix; Germann, G.; Leimer, Uwe

doi:10.1016/j.jcyt.2014.01.007

Cited by 33 publications

(33 citation statements)

References 34 publications

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“…Moreover, PRP reduced the ADSCs doubling time, and increased the slope value for cell proliferation. Similar effects were also observed in previous studies on ADSC culture (Kocaoemer et al, 2007;Koellensperger et al, 2014;Van Pham et al, 2013a) and in other MSC culture (Iudicone et al, 2014;Pham et al, 2014). The increased proliferation rate is thought to originate from possible combinatorial effects of the growth factor pool within PRP.…”

Section: Discussionsupporting

confidence: 85%

Good manufacturing practice-compliant isolation and culture of human adipose derived stem cells

Pham¹,

Vu²,

Le³

et al. 2014

Biomed Res Ther

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Abstract-Adipose-derived stem cells (ADSCs) are excellent for regenerative medicine. Like mesenchymal stem cells, ADSCs possess multi-potent differentiation capacity that enables them to differentiate into osteoblasts, chondrocytes and adipocytes, as well as trans-differentiation into other cells. ADSC transplantation has gained attention in recent years, especially in vitro expanded ADSC transplantation. This study aimed to provide a new method to in vitro primarily culture and secondary culture of ADSCs that were compliant with good manufacturing practice for clinical applications. Stromal vascular fraction (SVF) was extracted from adipose tissue by commercial kits. SVF was expanded in vitro in medium with non-allogeneic supplements. Cultured ADSCs maintained immune-phenotype, karyotype, and differentiation potential after ten passages. Moreover, ADSCs at 15th passage could not form tumors in NOD/SCID mice. This research produced a suitable protocol for clinical applications of expanded ADSCs.

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Section: Discussionsupporting

confidence: 85%

Good manufacturing practice-compliant isolation and culture of human adipose derived stem cells

Pham¹,

Vu²,

Le³

et al. 2014

Biomed Res Ther

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“…Most of the included studies described protocols relying on the platelets lyse after freeze-thaw cycles to obtain VBDs. Bone marrow mesenchymal stem cells (BMSC) were presented in most of this studies (Table 5); adipose stem cells -ASC (24,(41)(42)(43)(44)(45)(46), dental pulp stem cells -DPSC (47,48), umbilical cord stem cells -UCST (22,25,(49)(50)(51)(52) and orbital fat-derived stem cells (53) were also tested. VBD concentrations added to culture medium ranged from 0.5 to 30% (54)(55)(56).…”

Section: Resultsmentioning

confidence: 99%

Venous Blood Derivatives as FBS-Substitutes for Mesenchymal Stem Cells: A Systematic Scoping Review

et al. 2017

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Although the biological properties of mesenchymal stem cells (MSC) are well-characterized in vitro, MSC clinical application is still far away to be achieved, mainly due to the need of xenogeneic substances for cell expansion, such as fetal bovine serum (FBS). FBS presents risks regarding pathogens transmissions and internalization of animal's proteins, which can unleash antigenic responses in patients after MSC implantation. A wide range of venous blood derivatives (VBD) has been reported as FBS substitutes showing promising results. Thus, the aim of this study was to conduct a systematic scoping review to analyze whether VBD are effective FBS substitutes for MSC ex vivo expansion. The search was performed in SciVerse Scopus TM , PubMed, Web of Science TM , BIREME, Cochrane library up to January 2016. The keywords were selected using MeSH and entry terms. Two independent reviewers scrutinized the records obtained considering specific inclusion criteria. The included studies were evaluated in accordance with a modified Arksey and O' Malley's framework. From 184 found studies, 90 were included. Bone marrow mesenchymal stem cells (BMMSC) were presented in most of these studies. Overall, VBD allowed for either, maintenance of MCS's fibroblast-like morphology, high proliferation, high colony-formation ability and maintenance of multipotency. Besides. MSC expanded in VBD supplements presented higher mitogen activity than FBS. VBD seems to be excellent xeno-free serum for ex vivo expansion of mesenchymal stem cells. However, an accentuated heterogeneity was observed between the carried out protocols for VBD isolation did not allowing for direct comparisons between the included studies.

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“…This can be achieved, in part, by changing to chemically defined, xeno-free or human bloodderived alternatives (4,6). These human blood-derived alternatives include human serum, platelet-rich plasma, platelet-poor plasma and platelet lysate (7)(8)(9)(10). Studies comparing these alternatives make use of guidelines set out by the International Society of Cellular Therapy (ISCT) and International Fat Applied Technology Society for the characterisation of ASCs (11,12).…”

Section: Introductionmentioning

confidence: 99%

“…These guidelines propose a set of criteria that need to be met when expanding ASCs which include their ability to adhere to plastic, express a predefined surface marker profile (immunophenotype) and have the ability to differentiate into adipose tissue, bone and cartilage (11,12). In vitro studies that have used human alternatives for ASC expansion have described faster proliferation, better morphology and a comparable immunophenotype for certain surface markers (7)(8)(9)13,14). In contrast, these studies have also reported differing trilineage differentiation capacities.…”

Section: Introductionmentioning

confidence: 99%

Comparison of human platelet lysate alternatives using expired and freshly isolated platelet concentrates for adipose-derived stromal cell expansion

2018

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Pooled human platelet lysate (pHPL) has been used to expand adipose-derived stromal cells (ASCs) and can be formulated using fresh or expired buffy coats (BCs) which are then resuspended in either plasma or an additive solution. Not much is known about the effects that expired products and additive solutions have on ASC expansion, and the need for quality control and release criteria has been expressed. This pilot study compared proliferation, cell size, morphology and immunophenotype of ASCs expanded in the different pHPL alternatives versus foetal bovine serum (FBS). Quality control criteria were assessed prior to and during the manufacture of the pHPL alternatives. ASCs were then expanded in 1%, 2.5%, 5% or 10% of the different pHPL alternatives or in 10% FBS. Cell size, morphology, cell number and immunophenotype were measured using microscopy and flow cytometry. The majority of the pHPL alternatives were within the recommended ranges for the quality control criteria. ASCs expanded in the pHPL alternatives were smaller in size, displayed a tighter spindle-shaped morphology, increased cell growth and had a similar immunophenotype (with the exception of CD34 and CD36) when compared to ASCs expanded in FBS. Here we report on the effects that expired BC products and additive solutions have on ASC expansion. When taken together, our findings indicate that all of the pHPL alternatives can be considered to be suitable replacements for FBS for ASC expansion, and that expired BC products can be used as an alternative to fresh BC products. KeywordsAdditive solution, adipose-derived stromal cells, expired buffy coats, foetal bovine serum, pooled human platelet lysate History

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Choosing the right type of serum for different applications of human adipose tissue–derived stem cells: influence on proliferation and differentiation abilities

Cited by 33 publications

References 34 publications

Good manufacturing practice-compliant isolation and culture of human adipose derived stem cells

Good manufacturing practice-compliant isolation and culture of human adipose derived stem cells

Venous Blood Derivatives as FBS-Substitutes for Mesenchymal Stem Cells: A Systematic Scoping Review

Comparison of human platelet lysate alternatives using expired and freshly isolated platelet concentrates for adipose-derived stromal cell expansion

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