Good manufacturing practice-compliant isolation and culture of human adipose derived stem cells

Pham, Phuc Van; Vu, Ngoc Bich; Le, Dung Minh; Truong, Nhat Chau; Truong, Nhung Hai; Bui, Khanh; Phan, Ngoc Kim

doi:10.7603/s40730-014-0021-6

Cited by 16 publications

(16 citation statements)

References 40 publications

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“…To confirm the differentiation into chondroblasts, Safranin O staining was used; staining was positive. All results were similar to the previous publications Van Pham et al, 2016;Van Pham et al, 2014).…”

Section: Modulatists Bmmscs and Adscs Express The Msc Phenotypesupporting

confidence: 82%

“…ADSCs were isolated following the published protocol ( Van Pham et al, 2014). Briefly, adipose tissue was digested by collagenase using the ADSC Extraction kit (Geneworld Ltd, HCM, VN).…”

Section: Adsc Isolationmentioning

confidence: 99%

“…Mesenchymal stromal cells (MSCs) are multipotent progenitors derived from different sources, including bone marrow (BM) , adipose tissue ( Van Pham et al, 2014), umbilical cord (UC) blood (Sibov et al, 2012), UC (Van Pham et al, 2016), Wharton's jelly (Ducret et al, 2016), and other tissues (Ducret et al, 2016;Pelekanos et al, 2016;Sharpe, 2016). Although, bone marrow-derived MSCs (BMMSCs) are a popular source of MSCs for research and clinical applications, there are now alternative sources of MSCs, including adipose tissue-derived stem cells (ADSCs) and umbilical cord-derived mesenchymal stem cells (UCMSCs).…”

Section: Introductionmentioning

confidence: 99%

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Umbilical cord-derived stem cells (ModulatistTM) show strong immunomodulation capacity compared to adipose tissue-derived or bone marrow-derived mesenchymal stem cells

Pham¹,

Vu²,

Phan³

2016

Biomed Res Ther

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Abstract-Introduction:Mesenchymal stem cells (MSCs) show great promise in regenerative medicine. Clinical applications of MSCs have recently increased significantly, especially for immune diseases. Autologous transplantation is considered a safe therapy. However, its main disadvantages are poor stability and quality of MSCs from patient to patient, and labor-intensive and time-consuming culture procedures. Therefore, allogeneic MSC transplantation has recently emerged as a potential replacement for autologous transplantation. "Off the shelf" MSC products, or socalled "stem cell drugs", have rapidly developed; these products have already been approved in various countries, including Canada, Korea and Japan. This study aims to evaluate a new stem cell product or "drug", termed Modulatist TM , derived from umbilical cord mesenchymal stem cells (UCMSCs), which have strong immunomodulatory properties, compared to bone marrow-derived MSCs (BMMSCs) or adipose tissue-derived stem cells (ADSCs). Methods: Modulatist TM was produced from MSCs derived from whole umbilical cord (UC) tissue (which includes Wharton's jelly and UC), according to GMP compliant procedures. Bone marrow-and adipose tissue-derived MSCs were isolated and proliferated in standard conditions, according to GMP compliant procedures. Immunomodulation mediated by MSCs was assessed by allogenic T cell suppression and cytokine release; role of prostaglandin E2 in the immunomodulation was also evaluated. Results: The results showed that Modulatist TM exhibited stronger immunomodulation than BMMSC and ADSC in vitro. Modulatist TM strongly suppressed allogeneic T cells proliferation and decreased cytokine production, compared to BMMSCs and ADSCs. Conclusion: Modulatist TM is a strong immunomodulator and promising MSC product. It may be useful to modulate or treat autoimmune diseases.

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Section: Modulatists Bmmscs and Adscs Express The Msc Phenotypesupporting

confidence: 82%

“…ADSCs were isolated following the published protocol ( Van Pham et al, 2014). Briefly, adipose tissue was digested by collagenase using the ADSC Extraction kit (Geneworld Ltd, HCM, VN).…”

Section: Adsc Isolationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Umbilical cord-derived stem cells (ModulatistTM) show strong immunomodulation capacity compared to adipose tissue-derived or bone marrow-derived mesenchymal stem cells

Pham¹,

Vu²,

Phan³

2016

Biomed Res Ther

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“…They could also be differentiated into mesodermal origin cells such as osteoblasts and adipocytes. These results were presented by our group in the previous published study (Van Pham et al, 2014). …”

Section: Adsc Isolationsupporting

confidence: 75%

“…The digested adipose tissue suspension was centrifuged at 3500 rpm for 10 min to obtain the stromal vascular fraction (SVF). Finally, these SVF cells were washed and cultured as previous published procedure at 37°C, 5% CO2 (Van Pham et al, 2014). Medium was refreshed every 3 days.…”

Section: Adsc Isolation and Proliferationmentioning

confidence: 99%

Production of islet-like insulin-producing cell clusters in vitro from adiposederived stem cells

Dang¹,

Bui²,

Pham³

et al. 2015

Biomed Res Ther

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Abstract-Diabetes mellitus is a high incidence disease that has increased rapidly in recent years. Many new therapies are being studied and developed in order to find an effective treatment. An ideal candidate is stem cell therapy. In this study, we investigated the differentiation of adipose derived stem cells (ADSCs) into pseudo-islets in defined medium in vitro, to produce large quantities of insulin-producing cells (IPCs) for transplantation. ADSCs isolated from adipose tissue were induced to differentiate into islet-like insulin-producing cell clusters in vitro by inducing medium DMEM/F12 containing nicotinamide, N2, B27, bFGF, and insulin-transferrin-selenite (ITS). Differentiated cells were analyzed for properties of IPCs, including storage of Zn 2+ by dithizone staining, insulin production by ELISA and immunochemistry, and beta cell-related gene expression by reverse transcriptase PCR. The results showed that after 2 weeks of differentiation, the ADSCs aggregated into cell clusters, and after 4 weeks they formed islets, 50-400 micrometers in diameter. These islet cells exhibited characteristics of pancreatic beta cells as they were positive for dithizone staining, expressed insulin in vitro and C-peptide in the cytoplasm, and expressed pancreatic beta cellspecific genes, including Pdx-1, NeuroD, and Ngn3. These results demonstrate that ADSCs can be used to produce a large number of functional islets for research as well as application.

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Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review

Dongen

Tuin

Spiekman

et al. 2017

J Tissue Eng Regen Med

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Intraoperative application of the stromal vascular fraction (SVF) of adipose tissue requires a fast and efficient isolation procedure of adipose tissue. This review was performed to systematically assess and compare procedures currently used for the intraoperative isolation of cellular SVF (cSVF) and tissue SVF (tSVF) that still contain the extracellular matrix. Pubmed, EMBASE and the Cochrane central register of controlled trials databases were searched for studies that compare procedures for intraoperative isolation of SVF (searched 28 September 2016). Outcomes of interest were cell yield, viability of cells, composition of SVF, duration, cost and procedure characteristics. Procedures were subdivided into procedures resulting in a cSVF or tSVF. Thirteen out of 3038 studies, evaluating 18 intraoperative isolation procedures, were considered eligible. In general, cSVF and tSVF intraoperative isolation procedures had similar cell yield, cell viability and SVF composition compared to a nonintraoperative (i.e. culture laboratory-based collagenase protocol) control group within the same studies. The majority of intraoperative isolation procedures are less time consuming than nonintraoperative control groups, however. Intraoperative isolation procedures are less time-consuming than nonintraoperative control groups with similar cell yield, viability of cells and composition of SVF, and therefore more suitable for use in the clinic. Nevertheless, none of the intraoperative isolation procedures could be designated as the preferred procedure to isolate SVF.

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Good manufacturing practice-compliant isolation and culture of human adipose derived stem cells

Cited by 16 publications

References 40 publications

Umbilical cord-derived stem cells (ModulatistTM) show strong immunomodulation capacity compared to adipose tissue-derived or bone marrow-derived mesenchymal stem cells

Umbilical cord-derived stem cells (ModulatistTM) show strong immunomodulation capacity compared to adipose tissue-derived or bone marrow-derived mesenchymal stem cells

Production of islet-like insulin-producing cell clusters in vitro from adiposederived stem cells

Comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review

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