2,4,6-trinitrobenzene sulfonic acid-induced chronic colitis with fibrosis and modulation of TGF-β1 signaling

Loeuillard, Emilien; Bertrand, Julien; Herranen, Anni; Melchior, Chloé; Guérin, Charlène; Coëffier, Moı̈se; Aziz, Moutaz; Déchelotte, Pierre; Savoye, Guillaume; Marion‐Letellier, Rachel

doi:10.3748/wjg.v20.i48.18207

Cited by 19 publications

(20 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TNBS is a haptenizing agent that induces a Th1-type response in the murine colon, whereas DSS is thought to have a toxic effect on the intestinal epithelial cells, which compromises the integrity of the mucosal barrier and induces inflammation independent of the acquired immune system 19 , 28 - 30 . While both of these chemical agents have been used to create chronic-like IBD phenotypes 19 , 31 , we found that the mortality rates were lower in animals ingesting DSS in the drinking water compared to mice exposed with repetitive rectal TNBS administrations (unpublished results). After the DSS exposure, we allowed the animals 2-weeks recovery time which phenotypically resulted in a quiescent phase of chronic colitis.…”

Section: Discussionmentioning

confidence: 79%

Assessment of Inflammation in an Acute on Chronic Model of Inflammatory Bowel Disease with Ultrasound Molecular Imaging

Machtaler¹,

Knieling²,

Luong³

et al. 2015

Theranostics

View full text Add to dashboard Cite

Background: Ultrasound (US) molecular imaging has shown promise in assessing inflammation in preclinical, murine models of inflammatory bowel disease. These models, however, initiated acute inflammation on previously normal colons, in contrast to patients where acute exacerbations are often in chronically inflamed regions. In this study, we explored the potential of dual P- and E-selectin targeted US imaging for assessing acute inflammation on a murine quiescent chronic inflammatory background.Methods: Chronic colitis was induced using three cycles of 4% DSS in male FVB mice. Acute inflammation was initiated 2 weeks after the final DSS cycle through rectal administration of 1% TNBS. Mice at different stages of inflammation were imaged using a small animal ultrasound system following i.v. injection of microbubbles targeted to P- and E-selectin. In vivo imaging results were correlated with ex vivo immunofluorescence and histology.Results: Induction of acute inflammation resulted in an increase in the targeted US signal from 5.5 ± 5.1 arbitrary units (a.u.) at day 0 to 61.0 ± 45.2 a.u. (P < 0.0001) at day 1, 36.3 ± 33.1 a.u. at day 3, returning to levels similar to control at day 5. Immunofluorescence showed significant increase in the percentage of P- and E-selectin positive vessels at day 1 (P-selectin: 21.0 ± 7.1% of vessels; P < 0.05; E-selectin: 16.4 ±3.7%; P < 0.05) compared to day 0 (P-selectin: 10.3 ± 5.7%; E-selectin: 7.3 ± 7.0%).Conclusions: Acute inflammation can be accurately measured in a clinically relevant murine model of chronic IBD using ultrasound molecular imaging with a dual P- and E- selectin-targeted contrast agent.

show abstract

Section: Discussionmentioning

confidence: 79%

Assessment of Inflammation in an Acute on Chronic Model of Inflammatory Bowel Disease with Ultrasound Molecular Imaging

Machtaler¹,

Knieling²,

Luong³

et al. 2015

Theranostics

View full text Add to dashboard Cite

show abstract

“…3F). TGF-β is well known to play an important role in intestinal fibrosis by inducing collagen production in fibroblast and intestinal smooth muscle cells [34]. mRNA analysis of TGF-β showed a reduced level in TOKI treated mice when compared with the DSS control mice (Fig.…”

Section: Discussionmentioning

confidence: 92%

Toki-shakuyaku-san, a Japanese kampo medicine, reduces colon inflammation in a mouse model of acute colitis

Sreedhar

Arumugam

Thandavarayan

et al. 2015

International Immunopharmacology

View full text Add to dashboard Cite

“…The usage of DSS is dictated by its strong, rapid, and dose-dependent UC-like effect [ 72 ]. TNBS is widely used to trigger a CD-like colitis [ 73 ]. These models do not fully reflect human IBD; nevertheless, up to now, they are the best known colitis stimuli [ 74 ].…”

Section: Probiotics Treatment and Protection From Ibdmentioning

confidence: 99%

The Effectiveness of Probiotics in the Treatment of Inflammatory Bowel Disease (IBD)—A Critical Review

2020

View full text Add to dashboard Cite

Inflammatory bowel disease (IBD), which affects millions of people worldwide, includes two separate diseases: Crohn’s disease (CD) and ulcerative colitis (UC). Although the background (chronic inflammatory state) and some of the symptoms of CD and UC are similar, both diseases differ from each other. It is becoming clear that a combination of many factors, in particular genetic background, host immune response and microbial reduced diversity status are associated with IBD. One potential strategy to prevent/treat IBD is gut modulation by probiotics. Over the last twenty years, many publications have focused on the role of probiotics in the course of IBD. The review discusses the utility of different strains of probiotics, especially Bifidobacterium spp., in all factors potentially involved in the etiology of IBD. The probiotic modulatory properties among different study models (cell lines, animal models of colitis, clinical study) are discussed and probiotic usefulness is assessed in relation to the treatment, prevention, and remission of diseases.

show abstract

2,4,6-trinitrobenzene sulfonic acid-induced chronic colitis with fibrosis and modulation of TGF-β1 signaling

Abstract: Rats with TNBS-induced chronic colitis exhibited colon fibrosis associated with higher TGF-β signaling. Proteasome inhibition by bortezomib had no effect on fibrosis in our experimental conditions.

Cited by 19 publications

References 21 publications

Assessment of Inflammation in an Acute on Chronic Model of Inflammatory Bowel Disease with Ultrasound Molecular Imaging

Assessment of Inflammation in an Acute on Chronic Model of Inflammatory Bowel Disease with Ultrasound Molecular Imaging

Toki-shakuyaku-san, a Japanese kampo medicine, reduces colon inflammation in a mouse model of acute colitis

The Effectiveness of Probiotics in the Treatment of Inflammatory Bowel Disease (IBD)—A Critical Review

Contact Info

Product

Resources

About