Viability and vigour in seeds of Poincianella pyramidalis (Tul.) L. P. Queiroz by tetrazolium test

Abstract

-The Caatinga has a wide diversity of species with potential for exploitation, among which is the catingueira (Poincianella pyramidalis (Tul.) L. P. Queiroz), which is notable for its importance in the areas of timber and forestry. The use of rapid tests in quality control programs for seeds is an essential tool for evaluating physiological quality. The aim of this study was therefore to develop procedures for a tetrazolium test to evaluate viability and vigour in seeds of P. pyramidalis. Two procedures for preconditioning the seeds were tested: between moist paper towels and directly in water. In preparing the seeds, both manual scarification and cutting the seed at the opposite end to the hilum were tested. For staining, two concentrations of tetrazolium solution were used (0.075% and 0.15%) and three staining periods (30, 60 and 90 minutes), with the seeds being sorted into classes of viability and vigour. The experimental design was completely randomised in a 3 x 3 x 2 factorial scheme (harvest year x staining time x tetrazolium concentration), with four replications of 25 seeds. The tetrazolium test was efficient in assessing viability and vigour in seeds of P. pyramidalis. After cutting the opposite end to the hilum, the seeds were preconditioned directly in water for 24 hours at 25 °C, when the integument could be removed. Staining was achieved by immersing the seeds in a 0.075% tetrazolium solution for 90 minutes at 41 °C.

CONSERVATION OF SEEDS OF Myrcianthes pungens (Berg) Legr. IN DIFFERENT PACKAGING IN A CONTROLLED ENVIRONMENT1

“…The germination test has some limitations on its accomplishment, such as delays and possible modifi cations of the results due to the presence of fungi. Thus, it is necessary to use fast and reliable tests to estimate the viability of storage seeds (Cripa et al, 2014), such as the tetrazolium test, which is based on the activity of the dehydrogenase enzymes involved in the respiration process (Sousa et al, 2017).…”

EVALUATION OF VIABILITY OF Tabebuia aurea SEEDS THROUGH TETRAZOLIUM TEST

“…Ducke), which showed greater viability at the tetrazolium salt concentration of 0.075% for 4 hours, at temperatures of 35 or 40 °C (PEREIRA et al, 2020), and for Poincianella pyramidalis (Tul.) L. P. Queiroz, for which the best results were obtained using the tetrazolium salt concentration of 0.075% for 91 minutes at 41 °C (SOUSA et al, 2017). 1 *Means followed by the same uppercase letter (A, B, C) in the row and lowercase letter (a, b, c) in the column do not differ significantly by Tukey test at 5% probability level.…”

VIABILIDADE DE SEMENTES DE Senegalia polyphylla PELO TESTE DO TETRAZÓLIO

“…A pesquisa se torna relevante devido a avaliação da qualidade de sementes ser foco de pesquisas em tecnologia de sementes desde a década de 70, em busca de desenvolver e/ou aperfeiçoar metodologias que possibilitem obter resultados confiáveis quanto à viabilidade das mesmas em tempo inferior ao do teste padrão de germinação (Moore, 1972;Grabe, 1976;Vieira e Carvalho, 1994;McDonald, 1998). Um dos testes com tempo comprovadamente abreviado é o teste de tetrazólio (Oliveira et al, 2005;Ferreira et al, 2007;Deminicis et al, 2009;Sousa et al, 2017;Carvalho et al, 2017), desenvolvido por Delouche et al (1976), que permite, ainda, detectar danos por secagem, insetos, umidade, além de danos mecânicos provenientes da colheita e/ou beneficiamento (Brasil, 2009).…”

“…Em sementes florestais da Caatinga, algumas espécies foram testadas, como a Erythrina velutina Willd (Cunha;Gomes, 2015), a Poincianella pyramidalis (Tul.) L. P. Queiroz (Sousa et al, 2017) e a Anadenanthera peregrina (L.) Speg (Pinho et al, 2011), em que foi identificado que o tempo de imersão na solução e as concentrações testadas são fatores variáveis entre espécies para a condução do teste.…”