EVALUATION OF VIABILITY OF Tabebuia aurea SEEDS THROUGH TETRAZOLIUM TEST

Abstract

The viability of T. aurea seeds is evaluated through the germination test, taking 21 days, which makes it difficult to obtain quick information about the viability of its seeds. In this context, using the tetrazolium test as an evaluation method would be appropriate because it provides faster and more reliable information for several species. In view of the above, the objective of this study was to adapt the tetrazolium test methodology to evaluate the viability of T. aurea seeds. Initially, the imbibition curve was constructed to determine the appropriate hydration period for the seeds and the germination test was conducted, both for evaluating the initial quality and for comparison with the tetrazolium test results. For the tetrazolium test, the seeds were hydrated for 24 hours, then their coats were removed and the seeds were immersed in tetrazolium solutions. The concentrations used were 0.05, 0.075 and 0.1% for three staining periods 2, 4 and 6 hours, at 35 and 40 °C, in the absence of light. The experimental design was completely randomized in a 3 x 3 + 1 factorial scheme (three concentrations of tetrazolium solution x three staining periods + one control = germination test) with four replicates of 25 seeds. Tetrazolium test was adequate to evaluate the viability of T. aurea seeds using the concentration of 0.05% for four hours at 40 °C.

Physiological Quality of Stored Dipteryx alata Vogel Seeds: Tetrazolium Test and Radiographic Imaging Standardization

“…In a study by Brito et al (2020), in order to evaluate the vigor of Tabebuia aurea seeds under conditions similar to those described for baru seeds, the authors showed that the best concentration was 0.05% of the tetrazolium salt for four hours of soaking. For these authors, the tetrazolium test has become increasingly efficient in the methodologies for assessing the vigor of forest species.…”