Characterizing the Mechanism of Quetiapine Distribution in Lipid-Core Nanocapsules Pseudo-Phases Using a Validated Lc/Uv Method

Pharmacokinetic Investigation of Quetiapine Transport across Blood–Brain Barrier Mediated by Lipid Core Nanocapsules Using Brain Microdialysis in Rats

“…Total drug content and encapsulation efficiency of QTP in QLNC formulation were accessed using a validated LC/UV method . For total drug content measurement, QTP was extracted from QLNC through dilution in methanol (1:100) followed by ultrasonic bath for 20 min.…”

“…QTP hemifumarate salt was converted into the quetiapine free base through a liquid−liquid extraction procedure with ethyl acetate/ammoniun hydroxide saturated solution (50%, v/ v). 17 The ethyl acetate phase was concentrated under reduced pressure to remove the solvents, and the faint yellow oily material was placed into a desiccator cabinet protected from light. Samples were analyzed 72 h after the extraction procedure by infrared spectroscopy to confirm fumaric acid removal (data not shown).…”

“…Total drug content and encapsulation efficiency of QTP in QLNC formulation were accessed using a validated LC/UV method. 17 For total drug content measurement, QTP was extracted from QLNC through dilution in methanol (1:100) followed by ultrasonic bath for 20 min. Aliquots of 1000 μL of the obtained samples were centrifuged at 12.000 rpm for 10 min, the supernatant was filtered (0.45 μm), and 35 μL was injected into the LC system.…”

“…Therefore, the present work aims to evaluate if LNC type III carry the drug through the BBB. For that purpose, QTP was chosen as test compound due to its physicochemical properties (logD 7,4 and logP), which lead to the formation of a LCN with higher percentage of the drug distributed into the core of the particle . Furthermore, QTP is described as a substrate for efflux and influx transporters at BBB, , which might provide information regarding ability of LNC to bypass membrane transporters.…”

Validation of A High Performance Liquid Chromatography Method to Quantify Thymol in Nanocapsules of Bioactive Essential Oil from Lippia Sidoides

“…Thus the essential oil of L. sidoides was encapsulated in polymeric nanocapsules in order to decrease the toxic effects and maintain or increase its therapeutic efficiency, because this is one of the purposes of nanoencapsulation [10]. High-performance liquid chromatography is one of the quantification techniques commonly used for drug analysis in the pharmaceutical industry, and has been a widely used methodology in the analysis of conveyed drugs in nanocarriers [11][12][13] For essential oils, gas chromatography analysis techniques are generally employed, however, thymol, which is the major component and the pharmacological marker in L. sidoides essential oil, has an absorption spectra in the ultraviolet region. This fact allows the quantification of thymol in essential oils, plant extracts and nanocarriers by HPLC-UV and HPLC-DAD, as has been reported in several studies.…”