Validation of A High Performance Liquid Chromatography Method to Quantify Thymol in Nanocapsules of Bioactive Essential Oil from Lippia Sidoides

Louchard, Bianca Oliveira; Costa, Larissa Coelho; Silva, Angelo Roncalli Alves e; Leal, Luzia Kalyne Almeida Moreira

doi:10.15406/ijcam.2017.10.00330

IJCAM

2017

DOI: 10.15406/ijcam.2017.10.00330

|View full text |Cite

Validation of A High Performance Liquid Chromatography Method to Quantify Thymol in Nanocapsules of Bioactive Essential Oil from Lippia Sidoides

Bianca Oliveira Louchard¹,

Larissa Coelho Costa²,

Angelo Roncalli Alves e Silva³

et al.

Abstract: Materials and Methods Reagents, solvents and materials Thymol (99.5 % purity), PLGA (Poly D, L-lactide-co-Glycolyde 50:50) and poloxamer 188 (Poly(ethylene glycol)block-poly(propylene glycol)-block-poly(ethylene glycol) were purchased from Sigma Aldrich (São Paulo, USA), acetonitrile grade HPLC and acetone grade P.A. were purchased from Tedia (New Janeiro BR), water purified through milli-q filter system (Milipore, Brazil), phosphatidylcholine (Lipoid S-100) were purchased from Lipoid (Newark, USA), essential … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2020

2021

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 19 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

Desarrollo y validación de un método de cromatografía gaseosa para la determinación de mentol, salicilato de metilo, timol y ácido benzoico en solución antiséptica

Gola

Velázquez²,

Guerra

et al. 2020

Rev. Colomb. Cienc. Quím. Farm.

View full text Add to dashboard Cite

Se desarrolló y validó un método por cromatografía gaseosa para la cuantificación simultánea de mentol (MT), salicilato de metilo (SM), timol (TM) y ácido benzoico (AB) en una solución antiséptica bucal. Se utilizó una columna DB-WAX y dietilenglicol como estándar interno. Se determinó la linealidad en un intervalo de concentraciones de 64,00 a 96,00 μg/mL (MT), 80,00 a 120,00 μg/mL (SM), 96,00 a 144,00 μg/mL (TM) y 48,00 a 72,00 μg/mL (AB), obteniendo coeficientes de correlación superiores a 0,999, y coeficientes de variación de los factores de respuestas de 1,18, 1,95, 3,52 y 1,48%, respectivamente. Se establecieron límites de detección de 0,51, 1,14, 3,34 y 1,402 ng/mL para el MT, SM, TM y AB, respectivamente, mientras los límites de cuantificación fueron de 1,45, 3,43, 9,73 y 4,36 ng/mL en cada caso. Los porcentajes de recuperación fueron de 100,03, 99,31, 99,92 y 100,12; con coeficientes de variación de 0,42%, 0,79%, 0,66% y 0,76% para cada caso. El método fue lineal, exacto, preciso y selectivo para la determinación de los analitos en el control de calidad.

show abstract

Desarrollo y validación de un método de cromatografía gaseosa para la determinación de mentol, salicilato de metilo, timol y ácido benzoico en solución antiséptica

Gola

Velázquez²,

Guerra

et al. 2020

Rev. Colomb. Cienc. Quím. Farm.

View full text Add to dashboard Cite

show abstract

Voltammetric Determination of Isopropylmethylphenols in Herbal Spices

et al. 2021

View full text Add to dashboard Cite

Thymol and carvacrol—the components of herbal spices—are known for their broad biological activity as antimicrobials and antioxidants. For this reason, it is important to develop new methods for their determination in plant material. A simple, rapid, and sensitive method for determination of total content of these analytes in herbal spices using differential pulse voltammetry (DPV) has been developed. The basis of the research is the oxidation process of isopropylmethylphenols on a platinum microelectrode in glacial acetic acid containing acetonitrile (20%, v/v) and 0.1 mol L−1 sodium perchlorate as the supporting electrolyte. Linear voltammetric responses for thymol and carvacrol were obtained in a wide concentration range from 0.39–1105 and 0.47–640 µg mL−1, with a low detection limit of 0.04 and 0.05 µg mL−1, respectively. The analysis was performed using the multiple standard addition method. The results of the voltammetric determination are in good agreement with the data of the standard chromatographic method. To the best of our knowledge, this is the first presentation of an electrochemical procedure to determine these compounds in these environmental and electrode materials.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Validation of A High Performance Liquid Chromatography Method to Quantify Thymol in Nanocapsules of Bioactive Essential Oil from Lippia Sidoides

Cited by 2 publications

References 19 publications

Desarrollo y validación de un método de cromatografía gaseosa para la determinación de mentol, salicilato de metilo, timol y ácido benzoico en solución antiséptica

Desarrollo y validación de un método de cromatografía gaseosa para la determinación de mentol, salicilato de metilo, timol y ácido benzoico en solución antiséptica

Voltammetric Determination of Isopropylmethylphenols in Herbal Spices

Contact Info

Product

Resources

About