Seroprevalence of anti-Leishmania spp. antibodies in rural dogs from the city of Monte Negro, State of Rondônia, Brazil

Aguiar, Daniel Moura de; Oliveira, Trícia Maria Ferreira de Sousa; Cavalcante, Guacyara Tenório; Labruna, Marcelo B.; Camargo, Luís Marcelo Aranha; Machado, Rosângela Zacarias; Gennari, Solange Maria

doi:10.4322/rbpv.01901014

Cited by 6 publications

(5 citation statements)

References 10 publications

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“…Low agreement between the serological tests in our study can also be observed in researches carried out in Monte Negro -Rondônia (RO) State (AGUIAR et al, 2010), between ELISA and IFAT test, in Panorama -São Paulo (SP) State (LOPES et al, 2017), between ELISA and DPP tests. However, also high and moderate agreements have already been observed between the ELISA and IFAT tests in other localities of Brazil, as in Campo do Goycatazes -Rio de Janeiro State (TÁVORA et al, 2007) and Ilha Solteira (SP) (ASSIS et al, 2010;QUEIROZ et al, 2010).…”

Section: Discussionsupporting

confidence: 64%

Canine visceral leishmaniosis in an area of fishing tourism, Bonito Municipality, Mato Grosso do Sul, Central-West Brazil

et al. 2018

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The study aimed to investigate Leishmania infection in a population of dogs (92 animals) in a fishing area of Bonito Municipality, and evaluate comparatively the serological methods used, immunoenzymatic assay (ELISA), indirect immunofluorescence antibody test (IFAT), and rapid Dual Path-Platform test (DPP®). Blood and aspirate of bone marrow samples were used and parasitological investigation was also performed, such as parasite isolation in Neal, Novy, Nicolle (NNN) medium culture, Woo technique, Giemsa stained smears and specific identification by polymerase chain reaction (PCR). IFAT revealed 56/92 seropositive, the ELISA 8/92 and the DPP®41/92. Regarding the agreement between the serological tests by the Kappa index, there was a slight agreement between ELISA/IFAT and ELISA/DPP®, and fair agreement in IFAT/DPP®. The NNN culture was positive in three out of 21 dogs, and identified by PCR as Leishmania infantum chagasi. No samples were positive by the Woo technique. Our results showed low agreements between the serological tests recommended by the Brazilian Ministry of Heath, and it is necessary to associate other diagnostic techniques, such as parasitological tests and PCR, to increase the sensitivity for canine visceral leishmaniosis diagnosis, mainly regarding asymptomatic dogs in endemic areas.

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Section: Discussionsupporting

confidence: 64%

Canine visceral leishmaniosis in an area of fishing tourism, Bonito Municipality, Mato Grosso do Sul, Central-West Brazil

et al. 2018

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“…Aguiar et al (2010) matas, condições de habitação precárias, acúmulo de matéria orgânica e a presença de várias espécies de animais ao redor de casa como caprinos, ovinos, bovinos, suínos, equídeos e aves (Moreira Jr. et al 2003, Mestre & Fontes 2007. Baixas condições sanitárias e animais domésticos, principalmente cães, promovem a atração e concentração do vetor, bem como a prática da caça pelos assentados, normalmente noturna, aumenta o risco de infecção (Lainson & Rangel 2005.…”

Section: Discussionunclassified

Leishmaniose visceral em cães de assentamentos rurais

Silva

Melo

Costa³

et al. 2017

Pesq. Vet. Bras.

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RESUMO.-Nos assentamentos rurais há maiores concentrações de reservatórios e vetores da leishmaniose visceral canina (LVC) devido às transformações ambientais resultantes da ação antrópica pela ocupação do território, no entanto, não há estudos no Brasil acerca de informações epidemiológicas da LVC em assentamentos rurais. O objetivo do presente trabalho foi determinar a soroprevalência para Leishmania sp. em cães de assentamentos rurais, bem como identificar os fatores de risco associados a infecção. Foram utilizados 306 cães e o diagnóstico sorológico da LVC foi realizado através do ELISA S7. Dos 306 cães investigados, 118 apresentaram anticorpos anti-Leishmania sp., resultando em frequência de 38,6%. Idade de 12 -24 meses (OR=2,97), idade de 24--48 meses (OR=4,83), idade de 4-6 anos (OR=4,40), idade >6 anos (OR=3,62), contato com aves (OR=1,67) e sexo (fêmea) (OR=1,97) foram apontados como fatores de risco para LVC. Cães de assentamentos rurais do semiárido paraibano apresentaram frequência elevada de anticorpos anti-Leishmania sp., o que torna a população desses locais susceptíveis à infecção. Isso evidencia a necessidade de alerta aos órgãos de vigilância epidemiológica para estabelecer medidas de prevenção e controle dessa zoonose, incluindo ações educacionais e sanitárias nesses assentamentos, já que os mesmos estão situados em áreas com características propícias à instalação da LVC. In rural settlements there are higher concentrations of reservoirs and vectors of canine visceral leishmaniasis (CVL) due to environmental changes resulting from human action by occupation of the territory, however, there are no surveys in Brazil on epidemiological information of CVL in these areas. The aim of this study was to determine the seroprevalence of Leishmania sp. in dogs from rural settlements, as well as to identify the risk factors associated with infection. A total of 306 dogs were tested, and the serological diagnosis of CVL was performed by ELISA S7. Of the 306 dogs, 118 presented anti-Leishmania sp. antibodies., resulting in a frequency of 38.6%. The risk factors identified for CVL seropositivity were: age of 12-24 months (OR=2.97), age of 24-48 months (OR=4.83), age of 4-6 years (OR=4.40), age >6 years (OR=3.62), contact with poultry (OR=1.67) and gender (female) (OR=1.97). Dogs from rural settlements in the semiarid of Paraiba showed high anti-Leishmania sp. antibody frequency, what makes the population of those settlements susceptible to infection. This highlights for the need to alert the epidemiological surveillance agencies to stablish prevention and control methods for that zoonosis, including educational and health activities on these settlements, as they are located in areas with favorable characteristics for CVL installation.

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“…The expansion over time (Figure 1), as shown by VL-free areas in the first period (2001)(2002)(2003)(2004)(2005)(2006)) that reported vector, canine, or human cases in the second period (2007-2014), can be explained by simple adaptation of sandflies to varying temperatures and to the peridomiciles 21 , migratory movement of people with VL-contaminated dogs 22 , and to locals at the borders who have reported the disease 23 . Some states that were previously considered disease-free started to report the first autochthonous canine cases; for example Rondônia 24,25 23,34 .…”

Section: Discussionmentioning

confidence: 99%

Changes in the epidemiology of visceral leishmaniasis in Brazil from 2001 to 2014

Reis

Balieiro

Fonseca

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction: Visceral leishmaniasis (VL) is a neglected disease, with territorial expansion and regional differences in Brazil that require explanation. This study aimed to describe changes in the epidemiology of VL in Brazil from 2001 to 2014. Methods: The incidence rates, sociodemographic and clinical data, and case evolution were subgrouped from 2001 to 2006 and from 2007 to 2014 and presented descriptively. Spatial distribution of disease incidence rates and changes in the spatial and temporal pattern were examined. Results: In total, 47,859 VL cases were reported in Brazil between 2001 and 2014, with predominance in the Northeast macroregion (55%), though the incidence rate in this region declined between the two study periods. The State of Tocantins had the highest crude rate (26.2/100,000 inhabitants), which was responsible for VL increasing in the North macroregion. VL predominated in the urban zone (70%), in children under 4 years (34%); however, an increase in the incidence of VL in adults older than 40 years was identified, with 12.3% and 31% in the first and second period, respectively. The mapping of crude rates and autochthonous canine cases showed territorial expansion. The temporal distribution of VL was consistent in Brazil in general, with no pattern observed, but regional differences were found. Conclusions: The incidence of VL is increasing in Brazil. In addition to the State of Tocantins, which had the highest rate, new outbreaks of VL have occurred in the South macroregion of Brazil with small decreases identified in the incidence rate in the Northeast.

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Seroprevalence of anti-Leishmania spp. antibodies in rural dogs from the city of Monte Negro, State of Rondônia, Brazil

Cited by 6 publications

References 10 publications

Canine visceral leishmaniosis in an area of fishing tourism, Bonito Municipality, Mato Grosso do Sul, Central-West Brazil

Canine visceral leishmaniosis in an area of fishing tourism, Bonito Municipality, Mato Grosso do Sul, Central-West Brazil

Leishmaniose visceral em cães de assentamentos rurais

Changes in the epidemiology of visceral leishmaniasis in Brazil from 2001 to 2014

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