Effect of Propolis on Streptococcus mutans Biofilm Formation

Djais, Ariadna Adisattya; Putri, Nadhifa; Jemmy, Jemmy; Putri, Andin Rahmania; Darwita, Risqa Rina; Bachtiar, B. M.

doi:10.4034/pboci.2019.191.138

Cited by 9 publications

(7 citation statements)

References 18 publications

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“…In our study, we observed a lower efficacy of propolis extracts in C. pseudotuberculosis consolidated biofilms, most likely because bacteria in biofilms are inherently more tolerant to antimicrobial treatment when compared directly with planktonic cells of the same strain ( 53 ), and concentrations used herein were based on MIC 100 values obtained for planktonic cells. This result is similar to the finding by Djais et al ( 54 ), in which all concentrations of propolis extracts tested did not disrupt the Streptococcus mutans biofilm. Djais et al ( 54 ) attribute that increased in vitro production of biofilm can be related to the presence of sucrose, glucan, fructose, and other polysaccharides in the TSB culture medium, which provide the bacteria with enough substrate to initiate the formation of dental biofilms; thus, the in vitro C pseudotuberculosis greater resistance to the extracts can be also linked to factors related to bacterial culture in TSB broth.…”

Section: Discussionsupporting

confidence: 92%

“…This result is similar to the finding by Djais et al (54), in which all concentrations of propolis extracts tested did not disrupt the Streptococcus mutans biofilm. Djais et al (54) attribute that increased in vitro production of biofilm can be related to the presence of sucrose, glucan, fructose, and other polysaccharides in the TSB culture medium, which provide the bacteria with enough substrate to initiate the formation of dental biofilms; thus, the in vitro C pseudotuberculosis greater resistance to the extracts can be also linked to factors related to bacterial culture in TSB broth.…”

Section: Pseudotuberculosis Strainmentioning

confidence: 99%

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Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm

et al. 2021

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Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis in small ruminants, a chronic disease characterized by the development of granulomas in superficial and visceral lymph nodes as well as in several organs. An important characteristic of the infection with this bacterium is the formation of a biofilm and the absence of effective antibiotic therapy against the disease. From this scenario, the objective of this study was to evaluate the susceptibility of C. pseudotuberculosis to conventional antibiotics and to red, green, and brown propolis extracts obtained by the supercritical and ethanolic extraction methods as well as its activity in the bacterial biofilm. The results of the sensitivity test using antibiotics indicated a sensitivity of C. pseudotuberculosis strains to the antimicrobial agents. The ethanolic extract of green propolis and the supercritical red propolis extract showed the best antibacterial activities against planktonic C. pseudotuberculosis. A lower antimicrobial activity of the brown propolis extract was identified. Propolis extracts were effective in interfering with the formation of the C. pseudotuberculosis biofilm but had little activity on the consolidated biofilm. In conclusion, propolis extracts are more effective against C. pseudotuberculosis in the planktonic stage, being able to interfere with the formation of bacterial biofilm. However, the action of propolis extracts in a sessile and structured microbial biofilm is reduced.

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Section: Discussionsupporting

confidence: 92%

Section: Pseudotuberculosis Strainmentioning

confidence: 99%

Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm

et al. 2021

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“…Regarding balsam content, the values ranged from 0.57% to 1.58%. pH values comprised between 4.05 and 5.2 are one of the factors inhibiting the growth of microorganisms in propolis, and the values were similar with what was reported elsewhere [29][30][31]. Regarding moisture, which is an indication of the quality of propolis, all propolis samples showed values within the limit established by the Brazilian legislation (not more than 8%) [32].…”

Section: Physicochemical Characterization Of Propolis Samplessupporting

confidence: 86%

In Vitro Evaluation of Palestinian Propolis as a Natural Product with Antioxidant Properties and Antimicrobial Activity against Multidrug-Resistant Clinical Isolates

Daraghmeh

Imtara

2020

Journal of Food Quality

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The present work reveals, for the first time, the antioxidant and antibacterial properties of propolis samples collected from different regions of Palestine. The content on bioactive compounds has been estimated by total phenolic and flavone and flavonol content, while their antioxidant activity has been determined by radical scavenging methods of 1,2-diphenyl-1-picrylhydrazyl radical (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical (ABTS), and ferric reducing power assay (FRAP). The disc diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods were carried out to evaluate the antibacterial activity of Palestinian propolis against multidrug-resistant clinical isolates, including Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus faecalis. The results showed that the total phenolic content ranged from 9.62 mg to 124.94 mg gallic acid equivalent GAE/g of propolis, and the flavone and flavonol content ranged from 1.06 to 75.31 mg quercetin equivalent QE/g of propolis. The samples S6 from Al-Khalil presented the strongest radical scavenging activity toward DPPH, ABTS free radicals, and FRAP assay with IC50 values of 0.02, 0.03, and 0.05 mg/mL, respectively. The results of antibacterial activity indicated that the propolis samples inhibit the growth of Gram-positive strains better than Gram-negative ones. In addition, a strong correlation was observed between the pH, resin, balsam, total phenolic, flavones and flavonol, and total antioxidant capacity (TAC) from one side and the antibacterial activity of propolis samples except on Pseudomonas aeruginosa.

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“…While the amount of pinocembrin, galangin, and chrysin in the propolis was found to be higher than the present study, the CAPE was low. Moreover, Djais et al (9) found to have no effect of propolis on biofilm formation on S. mutans (ATCC 25175).…”

Section: Discussionmentioning

confidence: 99%

Determination of antimicrobial and anti-quorum sensing activities of water and ethanol extracts of propolis

Sorucu¹,

Ceylan

2021

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Quorum Sensing is a special system between bacteria to communicate with the signal molecules. Propolis is a resin collected from plants by honey bees. The present study examined anti-quorum sensing and antimicrobial activities of water and ethanol extracts of propolis. The antimicrobial activities of the propolis extracts were studied against Escherichia coli ATCC 25922 Gram (-), Listeria monocytogenes ATCC 7644, Streptococcus mutans CNCTC 8/77, Staphylococcus aureus ATCC 25923 Gram (+), Candida albicans ATCC 10239 (yeast) by the well diffusion method. Anti-quorum sensing activity, violacein inhibition activity, and anti-swarming activity were studied using Chromobacterium violaceum CV026 and C. violaceum ATCC 112472, and Pseudomonas aeruginosa (PA01) respectively. Both propolis extracts were detected antibacterial activity against Gram (+) bacteria and Candida albicans. However, antibacterial activity against Gram (-) bacteria were not observed. While the antimicrobial activity of water extract propolis was determined more effectively than ethanol extract, anti-quorum sensing and anti-swarming activities less effective. It was also detected that both extracts display violacein inhibition. It was revealed that the antimicrobial, anti-quorum sensing, anti-swarming activity depended on the dose of propolis extracts. Thirty-six phenolic compounds were analysed of propolis extract by HPLC-DAD. The amount and presence of phenolic compounds in the ethanol extract were more than the water extract apart from benzoic acid, cinnamic acid, syringic acid, (±) epigallocatechin, vitexin, rutin, and trans-chalcone. Consequently, the study put forward that the phenolic compounds of propolis have antibacterial, anti-quorum sensing, violacein inhibition, and anti-swarming activities changing depend on directly the extraction solvent and phenolic compounds.

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Effect of Propolis on Streptococcus mutans Biofilm Formation

Cited by 9 publications

References 18 publications

Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm

Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm

In Vitro Evaluation of Palestinian Propolis as a Natural Product with Antioxidant Properties and Antimicrobial Activity against Multidrug-Resistant Clinical Isolates

Determination of antimicrobial and anti-quorum sensing activities of water and ethanol extracts of propolis

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