Highly selective enrichment of aflatoxin B1 from edible oil using polydopamine-modified magnetic nanomaterials

Abstract

Aflatoxin B 1 (AFB 1 ) is a highly toxic mycotoxin that enters the human body through the food chain and poses a serious threat to human health. In this paper, polydopamine (PDA)-coated Fe 3 O 4 magnetic nanoparticles (Fe 3 O 4 @PDA MNPs) were prepared by the co-precipitation method to enrich aflatoxin from edible oil. Transmission electron microscopy (TEM), Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and vibrating sample magnetometer were used to characterize the Fe 3 O 4 @PDA MNPs. Using the obtained Fe 3 O 4 @PDA MNPs as an adsorbent, a simple method for enriching AFB 1 from samples by magnetic solid phase extraction (MSPE) combined with fluorescence rapid detection was developed. The effects of the ratio of Fe 3 O 4 MNPs to PDA, adsorption dosage, sample volume, adsorption time, and elution time on enrichment of AFB 1 were investigated to determine the optimal experimental conditions. This method has good intraday and daytime precision.

Recent Progress on Techniques in the Detection of Aflatoxin B1 in Edible Oil: A Mini Review

“…Many commonly used methods require sample preparation due to the different matrices of edible oil. Currently, a variety of methods are available for the extraction and isolation of mycotoxins from oil, such as liquid–liquid extraction or partitioning (LLE), frequently reported in the literature [ 101 , 102 , 103 , 104 , 105 ]; solid–phase extraction (SPE) [ 105 , 106 , 107 , 108 , 109 ]; immune affinity columns (IACs) [ 81 , 94 ]; IAC combined with dispersive liquid–liquid microextraction (DLLME) [ 91 ]; multifunctional cleanup columns [ 110 ]; the QuEChERS system [ 90 ]; gel permeation chromatography (GPC) [ 111 ]; immune assay extraction; and low-temperature cleanup (LTC) [ 112 , 113 , 114 , 115 ]. However, each method has its advantages and limitations.…”

High‐performance fabricated nano‐adsorbents as emerging approach for removal of mycotoxins: a review

“…In addition to the removal behaviour, the massive progress in the application of novel nano‐adsorbents in the extraction and enrichment of mycotoxins offered loading capabilities to various chromatographic methods for accurate determination of multi‐mycotoxins (Turan & Sahin, 2016; Sheng et al, 2021; Ma et al, 2021b). It can be predicted that the development of nano‐adsorbents will effectively promote the progress of precise detection technology for food safety.…”

The occurrence of aflatoxin M1 in doogh, kefir, and kashk in Hamadan, Iran

“…There are different types of aflatoxins, such as aflatoxin B 1 (AFB 1 ), B 2 (AFB 2 ), G 1 (AFG 1 ), and G 2 (AFG 2 ), although AFB 1 is the most toxic known mycotoxin (Sheng et al, 2021;Heshmati et al, 2021a). Aflatoxin M 1 (AFM 1 ) is a hydroxylated metabolite of AFB 1 created in animals that consumed foods containing AFB 1 and secreted in the milk (Nejad et al, 2019;Gonçalves et al, 2022;Heshmati et al, 2020;Jafari et al, 2021;Mohammadi et al, 2022).…”