Hypoglycemic effect of okra aqueous extract on streptozotocin-induced diabetic rats

Wu, Li; Weng, Minjie; Zheng, Hengguang; Lai, Pufu; Tang, Baosha; Chen, Junchen; Li, Yibin

doi:10.1590/fst.28619

Cited by 22 publications

(16 citation statements)

References 27 publications

(32 reference statements)

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“…Our findings that standardized aqueous crude extract of A. esculentus significantly reduce FPG in animals are similar to those reported in another study [47]. Conversely, the aqueous extract had no significant effect on body weight.…”

Section: Discussionsupporting

confidence: 92%

Total polyphenols and antihyperglycemic activity of aqueous fruits extract of Abelmoschus esculentus: Modeling and optimization of extraction conditions

et al. 2021

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Aqueous fruits extract of Abelmoschus esculentus (L.) Moench (Malvaceae) has been used traditionally in several communities to alleviate elevated blood glucose levels. However, optimized extraction conditions have not been reported. Thus, this study determined the optimal extraction conditions for extracting polyphenols from A. esculentus fruits and evaluated antihyperglycemic activity in vivo. Extraction time, temperature, and solid-to-solvent ratio were optimized using Response Surface Methodology (RSM). Total polyphenols and flavonoids were quantified using the Folin-Ciocalteu and aluminium chloride colorimetric methods, respectively. The fingerprint and quantification of quercetin—a major flavonoid with an antihyperglycemic effect was done using the chromatographic method. The antihyperglycemic activity was determined in a high-fat diet-Streptozotocin rat model. The rats were assigned to five groups (n = 6): Group 1 and 2 were normal and diabetic control received distilled water 1 mL/100g; Treatment group 3 and 4 received standardized A. esculentus fruit extract (AEFE) at a dose of 100 and 200 mg/kg, respectively; Group 5 received 5 mg/kg glibenclamide. All treatments were given orally for 14 days. Measurements of fasting plasma glucose (FPG) and body weight were done weekly. The RSM quadratic model predicted total polyphenols of 22.16 mg GAE/g DW. At optimal conditions of a solid-to-solvent ratio of 5%, extraction time 1 h, and extraction temperature of 70°C, confirmation experiments yield 20.2 [95% CI; 16.7 to 27.6] mg GAE/g DW, implying the model successfully predicted total polyphenols. The extract HPLC fingerprint showed 13 characteristic peaks with 0.45 ± 0.02 μg/g DW of quercetin. Compared with diabetic control, the standardized AEFE reduced FPG level dose-dependently (P < 0.001) with an EC50 of 141.4 mg/kg. Together, at optimal extraction conditions, extract with a high content of total polyphenols and good antihyperglycemic activity can be obtained. Studies are needed to identify additional polyphenolic compounds and determine their antidiabetic effects.

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Section: Discussionsupporting

confidence: 92%

Total polyphenols and antihyperglycemic activity of aqueous fruits extract of Abelmoschus esculentus: Modeling and optimization of extraction conditions

et al. 2021

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“…The method of preparation of okra aqueous extract and animal experiment according to the reference (Wu et al, 2020). Dried okra was extracted with distilled water at 1:25 (W/V).…”

Section: The Okra Aqueous Extract and Animal Experimentsmentioning

confidence: 99%

“…Through the comprehensive utilization of okra, the problems that the fresh okra fruits are different to preserve and are easily aging and affect the fruit quality are solved (Tang et al, 2017;Weng et al, 2017). Previous studies have confirmed that okra pods are rich in mucus (biological polysaccharides, sugar polymers), flavonoids, and dietary fiber, which have the effect of regulating glucose and lipid metabolism, promoting bowel movement, and preventing constipation (Georgiadis et al, 2011;Wu et al, 2020;Yuan et al, 2019). Diabetes mellitus (DM) is one of the most common diseases caused by metabolic disorders in humans.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the effect of okra extract on the diversity of intestinal flora in diabetic rats based on 16S rRNA sequence

Tang

Lai

et al. 2022

Food Sci. Technol

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To study the effect of okra aqueous extract on the intestinal flora of diabetic rats. Taking normal SD rats as CK group, diabetic rats induced by 40 mg/(kg•bw) streptozotocin (STZ) intraperitoneally as M group, normal and diabetic rats gavaged with 500 mg/(kg•bw) okra aqueous extract were CKO and MO groups,respectively. The bacterial flora of the cecum contents of rats was analyzed with 16S rRNA V3-V4 sequencing after 8 weeks of the experiment. Compared with CK, M increased Firmicutes 14.00% and decreased Proteobacteria 19.32% (P <0.01). Compared with M, MO down-regulated Lachnospiraceae 2.64% (such as Blautia, Anaerostipes), up-regulated Peptostreptococcaceae 1.20% in Firmicutes, and down-regulated Desulfovibrioceae 3.78% (such as Desulfovibrio) and up-regulated Burkholderia 5.24% (such as Parasutterella) in Proteobacteria. It is worth noting that MO up-regulated Christensenellaceae (LDA>4), which was negatively correlated with body mass index, inflammation, and metabolic syndrome. The okra aqueous extract reverses the changes of the intestinal flora of STZ-induced diabetic rats, which decreased Firmicutes and increased Proteobacteria by down-regulating Lachnospiraceae and Desulfovibrionaceae, up-regulating Burkholderiaceae, Christensenellaceae. The okra aqueous extract has the potential as a functional food with regulating intestinal microecology balance of diabetes.

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“…The existing studies suggest that the pathological mechanism of diabetic nephropathy is related to abnormal glucose metabolism pathway, changes in kidney hemodynamics, cytokines, inflammatory responses and genetic susceptibility factors and etc, and more and more scholars agree on the inflammatory response theory (Zhang et al, 2018;Dionísio et al, 2020a, b;Grom et al, 2020;Wu et al, 2020). As a natural focal disease, diabetes is accompanied by an obvious inflammatory response, which plays a crucial role in microvascular complications such as diabetic nephropathy, which has been verified in previous laboratory studies (Lovshin et al, 2017).…”

Section: Introductionmentioning

confidence: 80%

Clinical value of thalidomide on kk-rat model through TNF-a mediated inflammation approach

Huang

Duan²,

et al. 2022

Food Sci. Technol

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Hypoglycemic effect of okra aqueous extract on streptozotocin-induced diabetic rats

Cited by 22 publications

References 27 publications

Total polyphenols and antihyperglycemic activity of aqueous fruits extract of Abelmoschus esculentus: Modeling and optimization of extraction conditions

Total polyphenols and antihyperglycemic activity of aqueous fruits extract of Abelmoschus esculentus: Modeling and optimization of extraction conditions

Analysis of the effect of okra extract on the diversity of intestinal flora in diabetic rats based on 16S rRNA sequence

Clinical value of thalidomide on kk-rat model through TNF-a mediated inflammation approach

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