Application of platelet-rich plasma in the treatment of chronic skin ulcer - Case report

Pinto, Jony Arrais; Pizani, Natassia Soares; Kang, Hye Chung; Silva, Luis Augusto Knecht

doi:10.1590/abd1806-4841.20143004

Cited by 16 publications

(19 citation statements)

References 5 publications

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“…Successful completion of this trial will provide evidence of the best treatment strategy for patients with chronic ulcers. Results from this study can lead to a better understanding of the role of HA as a scaffold and that of the combined technique with PRP as a bio-stimulator in chronic ulcer treatment [2,14]. Overall, the results demonstrated that Group 2 (PRP + HA) represents a positive system for injured cells and tissue regeneration.…”

Section: Discussionmentioning

confidence: 84%

“…A correct approach is fundamental [1]. The Wound Bed Preparation guideline advises that after diagnosis the expert should correct the biological state of the ulcer micro-environment [2] based on TIME principles (Tissue, Infection, Moisture balance, Epidermal) [3]. The most important issue is to change the two discriminant factors: super-infection and exudation.…”

Section: Introductionmentioning

confidence: 99%

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Wound Healing: In Vitro and In Vivo Evaluation of a Bio-Functionalized Scaffold Based on Hyaluronic Acid and Platelet-Rich Plasma in Chronic Ulcers

Angelis

D'Autilio

Orlandi

et al. 2019

JCM

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Chronic ulcers are characterized by loss of substance without a normal tendency towards spontaneous healing. The Wound Bed Preparation Guideline advises that after diagnosis, the expert should correct the biological state of the ulcer micro-environment based on TIME principles (Tissue, Infection, Moisture balance, Epidermal). There are many ways to treat such ulcers, for example through use of advanced dressings, negative pressure, surgical toilets, dermal substitutes, autologous skin grafting, and free or local flaps. In vitro and in vivo pre-clinical models hold widely acknowledged potential yet complex limitations. Tissue bioengineering could be an ideal approach to foster innovative strategies in wound healing. Our observational study reports on an in vitro and in vivo evaluation of a bio-functionalized scaffold composed of platelet-rich plasma (PRP) and hyaluronic acid (HA) used in 182 patients affected by chronic ulcers (diabetic and vascular), comparing the results with a control group of 182 patients treated with traditional dressings (HA alone). After 30 days the patients who had undergone the combined treatment (PRP + HA), showed 96.8% ± 1.5% re-epithelialization, as compared to 78.4% ± 4.4% in the control group (HA only). Within 80 days, they had 98.4% ± 1.3% re-epithelialization as compared to 87.8% ± 4.1% in the control group (HA only; p < 0.05). No local recurrence was observed during the follow-up period. PRP + HA treatment showed stronger regenerative potential in terms of epidermal proliferation and dermal renewal compared with HA alone.

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Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Wound Healing: In Vitro and In Vivo Evaluation of a Bio-Functionalized Scaffold Based on Hyaluronic Acid and Platelet-Rich Plasma in Chronic Ulcers

Angelis

D'Autilio

Orlandi

et al. 2019

JCM

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“…PRP has a wide range of application in wound healing, cartilage, bone, musculoskeletal regeneration, oral surgery, dentistry, and cosmetics [8,9,10,11,12,13]. Moreover, PRP has been successfully applied in treating endometriosis, chronic skin ulcer and vitiligo in earlier studies [14,15]. Nevertheless, there are some drawbacks of using PRP [16].…”

Section: Introductionmentioning

confidence: 99%

The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method

Kardos

Simon

Vácz

et al. 2019

IJMS

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Autologous blood derived products, such as platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are widely applied in regenerative therapies, in contrast to the drawbacks in their application, mainly deriving from the preparation methods used. Eliminating the disadvantages of both PRP and PRF, hyperacute serum (HAS) opens a new path in autologous serum therapy showing similar or even improved regenerative potential at the same time. Despite the frequent experimental and clinical use of PRP and HAS, their protein composition has not been examined thoroughly yet. Thus, we investigated and compared the composition of HAS, serum, PRP and plasma products using citrate and EDTA by simple laboratory tests, and we compared the composition of HAS, serum, EDTA PRP and plasma by Proteome Profiler and ELISA assays. According to our results the natural ionic balance was upset in both EDTA and citrate PRP as well as in plasma. EDTA PRP contained significantly higher level of growth factors and cytokines, especially platelet derived angiogenic and inflammatory proteins, that can be explained by the significantly higher number of platelets in EDTA PRP. The composition analysis of blood derivatives revealed that although the preparation method of PRP and HAS were similar, the ionic and protein composition of HAS could be advantageous for cell function.

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“…PRP is a product derived from the centrifugation of whole blood that is rich in structural proteins and is used to promote the production of large amounts of growth factors, which stimulate collagen and extracellular matrix production with minimal amounts of plasma, which is an important alternative in cases where conventional treatments are not successful …”

Section: Introductionmentioning

confidence: 99%

Epidermal growth factor vs platelet‐rich plasma: Activity against chronic wound microbiota

Oliveira

Teixeira

et al. 2019

International Wound Journal

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The objective was to evaluate Staphylococcus aureus and Pseudomonas aeruginosa colonisation of wounds treated with recombinant epidermal growth factor (EGF) and platelet‐rich plasma (PRP); to analyse the susceptibility profiles of S. aureus and P. aeruginosa isolates from wounds treated with EGF and PRP; and to describe the presence of infection in EGF‐treated and PRP‐treated wounds. Experimental study was performed using clinical specimens collected with swabs. Patients were treated with PRP and EGF in the outpatient clinic of a university hospital. Forty‐three isolates were obtained from 31 patients, 41.9% (13/31) of whom had been treated with EGF and 58.0% (18/31) with PRP. Ten of the 43 isolates were identified as S. aureus, 60.0% (6/10) of which were isolated from PRP‐treated wounds. Among the 33 P. aeruginosa isolates, 66.6% (22/33) were isolated from PRP‐treated wounds. Regarding antimicrobial susceptibility, only one strain isolated from an EGF‐treated wound was identified as methicillin‐resistant S. aureus (MRSA). Among the P. aeruginosa isolates, one obtained from a patient treated with EGF was multidrug‐resistant. Patients treated with EGF had no infections during the follow‐up period, and there was a significant difference between the 1st and 12th week in wound infection improvement in patients treated with PRP (P = .0078).

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Application of platelet-rich plasma in the treatment of chronic skin ulcer - Case report

Cited by 16 publications

References 5 publications

Wound Healing: In Vitro and In Vivo Evaluation of a Bio-Functionalized Scaffold Based on Hyaluronic Acid and Platelet-Rich Plasma in Chronic Ulcers

Wound Healing: In Vitro and In Vivo Evaluation of a Bio-Functionalized Scaffold Based on Hyaluronic Acid and Platelet-Rich Plasma in Chronic Ulcers

The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method

Epidermal growth factor vs platelet‐rich plasma: Activity against chronic wound microbiota

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