Wound Healing: In Vitro and In Vivo Evaluation of a Bio-Functionalized Scaffold Based on Hyaluronic Acid and Platelet-Rich Plasma in Chronic Ulcers

Angelis, Bárbara De; D'Autilio, Margarida Fernandes Lopes Morais; Orlandi, Fabrizio; Pepe, Gerardo; Garcovich, Simone; Scioli, Maria Giovanna; Orlandi, Augusto; Cervelli, Valerio; Gentile, Pietro

doi:10.3390/jcm8091486

Cited by 78 publications

(83 citation statements)

References 46 publications

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“…Technologies have evolved from PDWHF to spraying technologies to deliver PRF or the use of combination products (i.e., PRP and Hyaluronan, Regen Lab SA, Mont-sur-Lausanne, Switzerland) [31]. At present, advanced therapies based on bioprinting technologies to create dermal constructs are being investigated.…”

Section: Discussionmentioning

confidence: 99%

Wound Dressing Selection Is Critical to Enhance Platelet-Rich Fibrin Activities in Wound Care

Amo

Perez‐Valle

Pérez-Zabala

et al. 2020

IJMS

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The use of platelet-rich fibrin (PRF) is investigated in ulcer management because it provides a healing milieu rich in growth factors and cytokines. Although crucial, the relevance of secondary dressings is under-researched and no data support the use of any particular dressing in preference to another. We assessed the properties of different dressing categories, including alginates, hydrocolloids, foams, hydrofibers, films, meshes and gauzes, in terms of affinity for PRF, releasate management (retention/extrusion) and the kinetics of cytokine release as well as the influence of each combination product, [PRF + dressing], on dermal cell behaviour, aiming to provide useful information for choosing the most adequate dressing for each particular patient. Active dressings including alginates, hydrofibers, foams and hydrocolloids blend with PRF, creating a diverse combination of products with different performances. Alginate and hydrofiber showed the highest affinity but moderate retention of releasate, without interfering with cell functions. Instead, the foam sequestered the releasate and hindered the release of growth factors, thereby compromising cell activities. Film and mesh presented very poor releasate retention and performed similarly to PRF by itself. Affinity index and releasate management explained 79% of platelet-derived growth factor (PDGF-BB) concentration variability, p < 0.001. Cell proliferation depended on the ability of the combination product to retain/release supernatant, PDGF-BB concentration and cell adhesion R 2 = 0.91, p = 0.014.

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Section: Discussionmentioning

confidence: 99%

Wound Dressing Selection Is Critical to Enhance Platelet-Rich Fibrin Activities in Wound Care

Amo

Perez‐Valle

Pérez-Zabala

et al. 2020

IJMS

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“…In wound healing, GFs may reduce bleeding and can accelerate healing time [12]. In vitro, the EGF, TGF-beta and FGF family, VEGF, granulocyte-macrophage colony-stimulating factor (GM-CSF), PDGF, connective tissue growth factor (CTGF), interleukin (IL) and the tumour necrosis factor-alpha (TNF-alpha) family can stimulate neoangiogenesis, cell recruitment, growth and morphogenesis, constituting a three-dimensional matrix that allows cellular arrangement into a correct three-dimensional organization [12,16]. For this reason, PRP may be considered a possible biostimulator.…”

Section: Discussionmentioning

confidence: 99%

“…Another mechanism that induces wound healing is the acceleration of hyaluronic acid (HA) production [12]. HA is a biopolymer identified in the ECM of skin, cartilage, bone and brain, among other tissues [22].…”

Section: Discussionmentioning

confidence: 99%

“…Combined treatment in chronic ulcers with PRP as a bio-stimulator and HA as a scaffold engaged in bio-functionalized scaffold could be considered as "gold standard" in vivo practice, especially where autograft or allograft tissue might not be available in sufficient quantity for reconstruction [12]. Several studies displaying the combined use of PRP and HA in wound healing have been reported [2,12].…”

Section: Discussionmentioning

confidence: 99%

“…MyCells ® A-PRP preparation involved centrifuging a patient's blood sample (10 mL). A vial of blood is centrifuged for 10 min at 3000 RPM [12].…”

Section: A-prp and Aa-prp Devicesmentioning

confidence: 99%

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Impact of the Different Preparation Methods to Obtain Autologous Non-Activated Platelet-Rich Plasma (A-PRP) and Activated Platelet-Rich Plasma (AA-PRP) in Plastic Surgery: Wound Healing and Hair Regrowth Evaluation

Gentile

Calabrese²,

Angelis

et al. 2020

IJMS

Self Cite

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Autologous therapies using platelet-rich plasma (PRP) need meticulous preparation—currently, no standardised preparation technique exists. Processing Quantitative Standards (PQSs) define manufacturing quantitative variables (such as time, volume and pressure). Processing Qualitative Standards (PQLSs) define the quality of the materials and methods of manufacturing. The aim of this review is to use existing PQSs and PQLs to report the in vivo/in vitro results obtained by using different Kits, that utilise different procedures (classified as Closed-Technique and Opened-Technique) to isolate autologous human activated (AA-PRP) or non-activated PRP (A-PRP). PQSs included the volumes of blood collected as well as the reagents used, the time/gravity of centrifugation, and the duration, temperature and tilt level/speed of centrifugation. PQLSs included the use of Calcium Chloride CaCl2, Kit weight, transparency of Kit components, the maintenance of a closed sterile processing environment and the use of a small centrifuge. Eight CE marked devices for PRP extraction were evaluated: Angel®, Biomed®, Cascade® and Selphyl®, Mag-18®, i-Stem®, MyCells® and Regenlab®. Using a Kit with the PQSs and PQLSs described in this study enables the isolation of A-PRP, thereby meeting consensus quality criteria. As our understanding of Critical Quality Attributes (CQAs) of A-PRP continues to evolve, especially with respect to purity and potency, adjustments to these benchmark PQSs and PQLs will hopefully help isolate A-PRP of desired CQAs with greater reproducibility, quality, and safety. Confirmatory studies will no doubt need to be completed.

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Platelet‐rich plasma enhances mechanical strength of strattice in rat model of ventral hernia repair

Fernandez-Moure

Eps

Scherba

et al. 2021

J Tissue Eng Regen Med

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Incisional hernia is a common complication of hernia repair despite the development of various synthetic and bio‐synthetic repair materials. Poor long‐term mechanical strength, leading to high recurrence rates, has limited the use of acellular dermal matrices (ADMs) in ventral hernia repair (VHR). Biologically derived meshes have been an area of increasing interest. Still these materials bring the risk of more aggressive immune response and fibrosis in addition to the mechanical failures suffered by the synthetic materials. Platelet‐rich plasma (PRP), a growth‐factor‐rich autologous blood product, has been shown to improve early neovascularization, tissue deposition, and to decrease the rates of recurrence. Here, we demonstrate that PRP promotes the release of growth factors stromal derived factor (SDF)‐1, transforming growth factor‐beta, and platelet‐derived growth factor in a dose‐dependent manner. Additionally, we utilize an aortic ring angiogenesis assay to show that PRP promotes angiogenesis in vitro. A rat model of VHR using StratticeTM ADM demonstrates similar findings in vivo, corresponding with the increased expression of vascular endothelial growth factor and collagen type 1 alpha 1. Finally, we show that the molecular and cellular activity initiated by PRP results in an increased mechanical stiffness of the hernia repair mesh over time. Collectively, these data represent an essential step in demonstrating the utility and the mechanism of platelet‐derived plasma in biomaterial‐aided wound healing and provide promising preclinical data that suggest such materials may improve surgical outcomes.

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Wound Healing: In Vitro and In Vivo Evaluation of a Bio-Functionalized Scaffold Based on Hyaluronic Acid and Platelet-Rich Plasma in Chronic Ulcers

Cited by 78 publications

References 46 publications

Wound Dressing Selection Is Critical to Enhance Platelet-Rich Fibrin Activities in Wound Care

Wound Dressing Selection Is Critical to Enhance Platelet-Rich Fibrin Activities in Wound Care

Impact of the Different Preparation Methods to Obtain Autologous Non-Activated Platelet-Rich Plasma (A-PRP) and Activated Platelet-Rich Plasma (AA-PRP) in Plastic Surgery: Wound Healing and Hair Regrowth Evaluation

Platelet‐rich plasma enhances mechanical strength of strattice in rat model of ventral hernia repair

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