Germinação e crescimento in vitro de Cattleya bicolor Lindley (Orchidaceae)

Suzuki, Rogério Mamoru; Almeida, Vanessa de Oliveira; Pescador, Rosete; Ferreira, Wagner de Melo

doi:10.1590/s2236-89062010000400004

Cited by 21 publications

(21 citation statements)

References 17 publications

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“…originated protocorms 10 days after in vitro inoculation. Suzuki et al (2010) verified that Cattleya bicolor Lindl. seeds germinated ca.…”

Section: Germination and Initial Developmentmentioning

confidence: 86%

Asymbiotic germination, multiplication and development of Alatiglossum fuscopetalum (Orchidaceae) as affected by culture medium, sucrose and growth regulators

Ferreira¹,

Vasconcelos²,

Silva³

et al. 2017

Iheringia Ser Bot

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-This investigation aimed at studying the germination and initial growth of Alatiglossum fuscopetalum (Hoehne) Baptista under in vitro conditions. The eff ects of Murashige/Skoog (MS and ½MS), Knudson (KN), and Vacin/Went (VW) culture media on the in vitro germination of A. fuscopetalum were analyzed. The infl uence of these media on the multiplication and initial growth of 90-day-old seedlings, and of diff erent sucrose, benzyladenine [BA] and indole-butyric acid [AIB] concentrations on the multiplication and growth of 120-day-old plants were also evaluated. The results revealed that ½MS was the most appropriate medium for germination. Regarding the growth of 90-day-old seedlings, MS provided the best outcome. The most eff ective medium for the multiplication and growth of 120-day-old plants was ½MS. Sucrose ranging from 1% to 3% favored plant growth. BA at 1 mg L -1 and IBA at 2 mg L -1 were the best growth regulator treatments for shoot proliferation and root growth, respectively.

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“…originated protocorms 10 days after in vitro inoculation. Suzuki et al (2010) verified that Cattleya bicolor Lindl. seeds germinated ca.…”

Section: Germination and Initial Developmentmentioning

confidence: 86%

Asymbiotic germination, multiplication and development of Alatiglossum fuscopetalum (Orchidaceae) as affected by culture medium, sucrose and growth regulators

Ferreira¹,

Vasconcelos²,

Silva³

et al. 2017

Iheringia Ser Bot

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“…Stewart & Kane (2006), estudando a germinação e o desenvolvimento inicial de Habenaria macroceratitis Willd., verificaram que não houve sequer a formação do protomeristema quando utilizaram esse meio de cultura, ocasionando a morte dos protocormos. Sorace et al (2008) Suzuki et al (2010) constataram em Cattleya bicolor Lindl. que o meio MS foi o que promoveu aumento significativo do número de folhas, das massas de matéria fresca e seca de caules e da massa de matéria seca de raízes.…”

Section: Crescimento In Vitro De Cattleya Harrisonianaunclassified

Germinação, crescimento e desenvolvimento in vitro de orquídeas (Cattleya spp., Orchidaceae)

Schneiders¹,

Pescador

Booz

et al. 2012

Rev. Ceres

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Germination, growth and development in vitro of orchids of the genus CattleyaOrchids are over-exploited in their natural environment due to their ornamental importance, which is leading some species to extinction. In vitro culture is an alternative for ex-situ conservation. Our objective was to determine an efficient medium for in vitro seed germination and initial seedling growth of Cattleya forbesii and Cattleya harrisoniana. Seeds of Cattleya forbesii were incubated in Murashige & Skoog (MS) medium (T1) and MS added with 2,5 g L -1 of activated charcoal (T2). For the last, plants with 1 ± 0,2 cm of height were submitted to the following treatments: T1, T2, MS reduced to the half strenght (T3) and MS reduced to the half strenght supplemented with 1,25 g L -1 of activated charcoal (T4). Thirty days after seed inoculation of C. forbesii, it was verified 45% and 90% of germination in T1 and T2, respectively. The addition of activated charcoal also promoted an increase in the height of the C. forbesii seedlings Germinação, crescimento e desenvolvimento in vitro de orquídeas (Cattleya spp., Orchidaceae)As orquídeas no ambiente natural sofrem exploração devido a sua importância ornamental, levando algumas espécies à extinção. O cultivo in vitro é uma forma alternativa para a conservação ex-situ. Procurou-se determinar um meio de cultura eficiente para a germinação in vitro de sementes e o crescimento inicial de plântulas de Cattleya forbesii, bem como para o crescimento de plântulas in vitro de Cattleya harrisoniana. No primeiro caso, sementes foram inoculadas em meio de cultura básico de Murashige & Skoog (MS) = T1 e MS básico acrescido de 2,5 g L -1 de carvão ativado (CA) = T2. No segundo, plântulas com 1 ± 0,2 cm de altura foram submetidas aos tratamentos T1, T2, MS com a metade da concentração original de macro-micronutrientes (T3) e MS com a metade da concentração original de macro-micronutrientes suplementado com 1,25 g L -1 de CA (T4). Verificou-se aos 30 dias em C. forbesii uma porcentagem de germinação de 45% em T1 e 90% em T2. A adição de CA ao meio de cultura trouxe aumento na altura de plântulas de C. forbesii de acordo com análises realizadas aos 180 dias de cultivo. Em relação ao crescimento de C. harrisoniana, aos 240 dias observou-se que todos os parâmetros médios avaliados (altura da parte aérea, massa de matéria fresca total, número de raízes e folhas, comprimento da maior raiz e diâmetro do pseudocaule) foram significativamente maiores em T2. Dessa forma, sugere-se o uso do meio MS acrescido de 2,5 g L -1 de CA (T2), uma vez que é significativamente favorável tanto para a germinação de sementes quanto para o crescimento de ambas as espécies.Palavras-chave: carvão ativado, meio de cultura, micropropagação, orquídeas.

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“…For commercial cultivation, using quality substrate, which supports the development of these flowering, becomes extremely important (Assis et al, 2010). However, orchids growth happens slowly (Suzuki et al, 2010), exposing the substrate for long periods to colonization by weeds such as Pilea microphylla (brilhantina), which affects seedlings development, increasing the cost of production and reducing their commercial value (Freitas et al, 2007b).…”

Section: Introductionmentioning

confidence: 99%

Chemical control of Pileamicrophylla in cattleya seedlings (Cattleyatenebrosa x Cattleyaleopoldy)

et al. 2014

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-Species of the orchidaceae family are grown for marketing flowers and among them the genus Cattleya stands out. However, due to its slow growth, the substrate is subjected to weed infestation. Therefore, this study aims to evaluate the selectivity and efficiency of herbicides in controlling Pilea microphylla in Cattleya orchid seedlings (Cattleya tenebrosa x Cattleya leopoldy). We used a completely randomized design with four replications. The evaluated herbicides were oxyfluorfen (0, 120, 240 and 480 g ha ) and metsulfuron-methyl (0, 1.2, 1.8 and 2.4 g ha -1). At post-emergence applying time, the Cattleya plants had three bulb sand were 10 cm tall, while P. microphylla, where 5 cm tall. Nicosulfuron, mesotrione and clethodim herbicides did not control P. microphylla, while oxyfluorfen and flumioxazin showed over 90% efficiency level sin controlling P. microphyllafrom 14 days after application (DAA). As to metsulfuron-methyl, it showed efficiency superior to 90% from the control dose of 1.8 g ha -1at 28 DAA. All herbicides were selective plants of Cattleya, however, only oxyfluorfen, flumioxazin and metsulfuronmethyl were effective in controlling P. microphylla.Keywords: weed, herbicide, floriculture, selectivity. ), flumioxazin (0, 12,5, 25 e 40 g ha -1 ), nicosulfuron (0, 20, 40 e 80 g ha -1 ), mesotrione (0, 96, 144 e 192 g ha -1 ), clethodim (0, 60, 84 e 108 g ha -1 ) e metsulfuron-methyl (0, 1,2, 1,8 RESUMO -Espécies da família

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Germinação e crescimento in vitro de Cattleya bicolor Lindley (Orchidaceae)

Cited by 21 publications

References 17 publications

Asymbiotic germination, multiplication and development of Alatiglossum fuscopetalum (Orchidaceae) as affected by culture medium, sucrose and growth regulators

Asymbiotic germination, multiplication and development of Alatiglossum fuscopetalum (Orchidaceae) as affected by culture medium, sucrose and growth regulators

Germinação, crescimento e desenvolvimento in vitro de orquídeas (Cattleya spp., Orchidaceae)

Chemical control of Pileamicrophylla in cattleya seedlings (Cattleyatenebrosa x Cattleyaleopoldy)

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