Asymbiotic germination, multiplication and development of Alatiglossum fuscopetalum (Orchidaceae) as affected by culture medium, sucrose and growth regulators

Abstract

-This investigation aimed at studying the germination and initial growth of Alatiglossum fuscopetalum (Hoehne) Baptista under in vitro conditions. The eff ects of Murashige/Skoog (MS and ½MS), Knudson (KN), and Vacin/Went (VW) culture media on the in vitro germination of A. fuscopetalum were analyzed. The infl uence of these media on the multiplication and initial growth of 90-day-old seedlings, and of diff erent sucrose, benzyladenine [BA] and indole-butyric acid [AIB] concentrations on the multiplication and growth of 120-day-old plants were also evaluated. The results revealed that ½MS was the most appropriate medium for germination. Regarding the growth of 90-day-old seedlings, MS provided the best outcome. The most eff ective medium for the multiplication and growth of 120-day-old plants was ½MS. Sucrose ranging from 1% to 3% favored plant growth. BA at 1 mg L -1 and IBA at 2 mg L -1 were the best growth regulator treatments for shoot proliferation and root growth, respectively.

Asymbiotic germination, initial development in vitro and acclimatization of Cyrtopodium paludicolum Hoehne, a Brazilian Savanna orchid species

“…Four microscope slides per replication containing protocorms/seedlings were prepared and analyzed by means of a dissecting microscope. The percentages of individuals obtained for each developmental stage and for each replication were multiplied by 1, 2, 3 and 4 (weights) according to their respective stages, so that the growth index could be calculated according to Spoerl (1948), as modified by Ferreira et al (2017). The growth index of each replication was the sum of all stages of their individuals.…”

“…The most adequate conditions for orchid seed germination and, consequently, seedling growth in vitro vary according to the genus and species (Suzuki et al 2009;Abrão et al 2014;Ferreira et al 2017), which demonstrates the importance of studies regarding culture medium composition, especially its mineral nutrients, carbohydrate concentration and hormonal makeup. Such studies would allow for a better understanding of the physiological processes involved in the development of these species.…”

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

“…It could be noted that Brazil has the highest number of published works both on propagation and germplasm conservation, while for some countries only researches referring to propagation of native orchid species are reported and for other countries (Guyana, Paraguay, Trinidad and Tobago, Suriname, Uruguay) there were no registered works in any of the topics. Almeida et al 2017;Caovila et al 2016;Cardoso and Ono 2011;Cavalcante Martini et al 2001;Corrêa Pereira et al 2011;2015;Da Silva et al 2014;Da Silva Júnior et al 2012;De Carvalho et al 2018;De Conti et al 2018;De Melo Ferreira et al 2017;De Menezes Gonçalves et al 2012;Dezan et al 2012;Dignart et al 2009;Dorneles and Trevelin 2011;Endres Júnior et al 2014;Favetta et al 2017;Galdiano Júnior et al 2011;2012a,b;Gomes de Araújo et al 2009;Hosomi et al 2017;Lando et al 2016;Lone et al 2010;Mantovani et al 2016;Massaro et al 2012;Monteiro do Rêgo et al 2009;Moraes et al 2005;Pasqual et al 2011;Pedroso-de-Moraes et al 2012;Pereira et al 2018;Pereira Gomes et al 2015;Prizão et al 2012;Rodrigues et al 2015;Rodrigues Guimarães et al 2013;Sasamori et al 2015;…”

CARACTERIZAÇÃO ANATÔMICA E HISTOQUÍMICA DA LÂMINA FOLIAR DE SIDA rhombifolia L.

“…As espécies de Orchidaceae, de uma maneira geral, são ornamentais e, devido a isso, possuem grande importância econômica e isso vem favorecendo a realização de coletas indiscriminadas das espécies dos seus ambientes naturais, os quais também vem sofrendo fortemente com a degradação por meio da ação antrópica. Sendo assim, as técnicas de propagação vegetativa, que permitem a produção de mudas em larga escala, podem ser uma excelente opção para atender as demandas comerciais e para programas de conservação que visem à reintrodução destas plantas na natureza (DUTRA et al, 2009;SUZUKI et al, 2012;FERREIRA et al, 2017).…”

“…Na micropropagação de orquídeas diferentes tipos de explantes podem ser utilizados como, por exemplo, os protocormos (GOMES et al, 2015) os quais podem ser seccionados e induzir a formação de "protocorm-like-bodies" (PBLs) sob a influência de reguladores de crescimento, principalmente das auxinas e citocininas (FRANCESCHI, 2013). A técnica é conhecida como "thin cell layer" (TCL) e consiste na excisão de diferentes órgãos da planta que são seccionados transversal ou longitudinalmente (NHUT et al, 2006).…”