Potential of Pandanus odoratissimus as a CNS depressant in Swiss albino mice

Abstract

In this study, several neuropharmacological effects of methanolic leaf extract of Pandanus odoratissimus (PO) (family; Pandanaceae) were studied in albino mice using various experimental models. The effect of PO on the CNS was studied by using different neuropharmacological paradigms including spontaneous motor activity, rota-rod performance and potentiation of Pentobarbital sodium sleeping time in albino mice. Preliminary phytochemical evaluation and acute toxicity studies were also carried out where LD 50 >2000 mg/kg was considered non-toxic through acute exposure in rats by the oral route. The methanolic leaf extract (50,100 and 200 mg/kg i. p.) produced a reduction in spontaneous motor activity, motor coordination and prolonged Pentobarbital sodium sleeping time. Preliminary qualitative chemical studies indicated the presence of steroids, saponins, terpinoids, glycosides, tannins, flavonoids and phenolics in the extract. These observations suggest that the leaf of Pandanus odoratissimus contains some active principles which possess potential CNS-depressant action Uniterms: Pandanus odoratissimus/pharmacognosy. CNS-depressant action/experimental study. Spontaneous motor activity. Rota-rod performance. Sodium pentobarbital/potentiation of sleeping time.Estudaram-se alguns efeitos neurofarmacológicos do extrato metanólico de Pandanus odoratissimus (PO) (família Pandanaceae) em camundongos albinos, usando vários modelos experimentais. O efeito do PO no SNC foi estudado por meio de diferentes paradigmas neurofarmacológicos, como atividade motora espontânea, desempenho na haste rotatória e a potenciação do tempo de sono em camundongos albinos pelo pentobarbital sódico. A avaliação fitoquímica preliminar e os estudos de toxicidade aguda foram realizados e a DL 50 >2000 mg/kg é considerada não tóxica, por meio da exposição aguda, por via oral, em ratos. O extrato metanólico de folha (50,100 e 200 mg/kg i. p.) produziu redução da atividade motora espontânea, da coordenação motora e tempo prolongado de sono pelo pentobarbital sódico. Estudos químicos qualitativos preliminares indicaram a presença de esteróide, saponinas, terpenóides, glicosídios, taninos, flavonóides e fenólicos no extrato. As observações sugerem que a folha de Pandanus odoratissimus contém alguns princípios ativos com atividade potencial como depressores do SNC. Unitermos:Pandanus odoratissimus/farmacognosia. Ação depressor do SNC/estudo experimental. Atividade motora espontânea. Desempenho na haste rotatória. Pentobarbital sódico/potenciação no tempo de sono.

Pharmacological Activities of Streptomyces Species PO-178 Isolated from Rhizosphere Soil of Agumbe, Karnataka, India

“…A reduction in the motor activity is considered to be depressant function of the drug. Gammaaminobutyric acid (GABA) is the major inhibitory neurotransmitter in CNS and different drugs act via GABA (Khatun et al, 2011;Raju et al, 2011;Sugavanam et al, 2012;Kazmi et al, 2012). In this study, the crude extract of isolate PO-178 showed reduction in locomotor activity but the activity was lower when compared to standard drug.…”

“…Spontaneous locomotor activity of Swiss Albino mice was studied using Actophotometer in order to evaluate CNS depressant activity of extract (Raju et al, 2011). The test groups, standard group and control group animals were administered with extract (100 and 200 mg/kg body weight), diazepam (5 mg/kg b.w) and normal saline (2 ml/kg b.w) respectively.…”

Hepatoprotective Effect of <i>Pandanus odoratissimus</i> L Inflorescence Extracts in Acetaminophen-treated Guinea Pigs

“…Chemical component analysis of the root parts led to the isolation of two phenolic compounds, four lignan type compounds plus a new benzofuran derivative which showed strong antioxidative activities [12]. P. odoratissimus L. (whole plant) exhibit significant antitumor and antioxidant activities [13]; and its leaves possess potential CNS-depressant action [14] as well as anti-inflammatory activity [15]. Moreover, the leaves are reported to have natural antioxidant properties [16,17] and moderate activity against gram-positive bacteria [15].…”

Comparative study of antidiarrheal activity of methanol extracts from leaf and fruit of Pandanus odoratissimus Linn

“…It is experimentally validated that P. odoratissimus possess antibacterial (Wei et al 2008;Kumar et al 2010;El-Shaibany 2014), anti-inflammatory (Londonkar et al 2010), antioxidant (Jong and Chau 1998;Londonkar and Kamble 2009;Sasikumar et al 2009;El-Shaibany 2014), antitumor (Panigrahi et al 2011), antidiabetic , antiepileptic (Adkar et al 2014), anti-hyperlipidemic (Zhang et al 2013), CNS depressant (Raju et al 2011), nocturnal enuresis (El-Shaibany 2014), hepatoprotective and hepatocurative (Ilanchezhian and Joseph 2010;Londonkar and Kamble 2011) activities. The principle constituent of P. odoratissimus oil, b-phenyl ethyl methyl ether, is responsible for the characteristic aroma of spadices.…”