Pharmacological Activities of Streptomyces Species PO-178 Isolated from Rhizosphere Soil of Agumbe, Karnataka, India

Prashith, Ktr; R, Onkarappa; Raghavendra, HL

doi:10.4314/star.v2i2.98892

Cited by 8 publications

(6 citation statements)

References 30 publications

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“…A and B ). This result similar to that obtained by Prashith et al, (2012) [16,17] who isolated Streptomyces isolate from Agumbe soils in India that able to depletion the growth of several bacterial and fungal species . KGL -13 : A-against bacterial test organisms (1-E. coli, 2-P. aeruginosa, S. aureus, M.…”

Section: Table (1): Numbers Of Actinomycetes Colonies Per Gram Growinsupporting

confidence: 88%

Screening for Antimicrobial Agents Producing Actinomycetes Isolated from Agricultural Soils in Hilla/Iraq

Jarallah¹

2014

IOSRJPBS

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Twelve agricultural soil samples were collected from different sites of Hilla city in Iraq . Out of these samples, 30 Actinomycetes isolates were isolated and purified in order to be tested for their antibiotics production ability . Primary screening exhibited there was one active isolate that posses antibacterial activity against four pathogenic bacterial species : Pseudomonas aeruginosa ,Escherichia coli , Morganella sp. and Staphyloccocus aureus , in addition to antifungal activity towards two fungal species : Candida albicans and Candida glabrata . This active isolate was encoded as Streptomyces KGL-13 ,then it was identified by culturing on different International Streptomycetes Project media (ISP-1 to ISP-7 ) and characterizing by several biochemical tests.partial purification of antimicrobial substances was done using ethyl acetate (at ratio 1:1) and evaporated at 45 ο C to get pasty mass. The zone of inhibition (ZOI ) diameter in mm of extract was determined on Muller Hinton agar .The results showed the highest ZOI happened towards E.coli and P. aeruginosa when gave 22 mm and 21 mm ,respectively. While it show a good activity against C.albicans ,but it had less activity towards C.glabrata.

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Section: Table (1): Numbers Of Actinomycetes Colonies Per Gram Growinsupporting

confidence: 88%

Screening for Antimicrobial Agents Producing Actinomycetes Isolated from Agricultural Soils in Hilla/Iraq

Jarallah¹

2014

IOSRJPBS

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“…A large number of globally threatened species of flora and fauna are found in the Western Ghats [38][39][40] . Bio activities such as antimicrobial, antioxidant, enzyme inhibitory, anthelmintic, insecticidal, cytotoxic, analgesic, antipyretic, anti-inflammatory and CNS depressant activity of soil actinomycetes from different parts of Western Ghats of Karnataka, India such as Thirthahalli, Agumbe, Mahishi, Kodachadri, Talakaveri, Dandeli and Kudremukh have been investigated 12,13,[41][42][43][44][45][46][47][48][49][50] . In the present study, bioactive Streptomyces species MPPO-02 isolated from rhizosphere soil of Mahishi, Karnataka, India displayed inhibitory activity against bacterial strains isolated from burn, dental caries and urinary tract infections.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Activity of Bioactive Streptomyces Species Mppo-02 Against Clinical Isolates of Burn, Dental Caries and Urinary Tract Infections

Manasa¹,

Sharma²,

R³

et al. 2014

J Biol Sci Opin

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The present study was conducted to determine antibacterial activity of ethyl acetate extract obtained from the fermentation broth of bioactive Streptomyces species MPPO-02 previously isolated from a rhizosphere soil of Mahishi, Karnataka, India. Agar well diffusion assay was performed to investigate inhibitory effect of ethyl acetate extract against a total of 15 clinical isolates from burn, dental caries and urinary tract infection. The extract was found inhibitory against all test bacteria but to a varied extent. Gram positive bacteria were found more susceptible to extract when compared to Gram negative bacteria. The isolate MPPO-02 can be exploited for the development of agents active against pathogenic microorganisms. Further studies on purification of active principles from solvent extract, their characterization and inhibitory activity are to be carried out.

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“…The selected actinomycetes isolates were grown in 10 mL M1 medium at 28°C for 48 h and confirmed as actinomycetes by Gram staining [21]. Actinomycetes were harvested from 1.5 mL of culture after centrifugation at 7500 x g for 10 min.…”

Section: Molecular Characterization Of the Selected Actinomycete Isolates Extraction Of The Actinomycetes Dnamentioning

confidence: 99%

Molecular Characterization of Four Antibiotic Producing Actinomycetes Isolated from Menengai Crater

Ltd¹

2020

PPPS

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Multi-drug resistant pathogens are a leading cause of human morbidity and mortality all over the world. This study aimed at isolating actinomycetes from the soils of Menengai crater and characterizing selected actinomycetes using morphological, biochemical, and molecular techniques. The study involved isolation of actinomycetes using Starch Casein (SC), Luria Bertani (M1) and Starch Nitrate (SN) agar media. The isolated actinomycetes were characterized using morphological and biochemical techniques. The screening for antibiotic activity was carried out via primary screening using perpendicular method and secondary screening using agar plug technique against Staphylococcus aureus (ATCC 25923), Streptococcus pneumoniae (ATCC 49617), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Proteus vulgaris (ATCC 49990), Alternaria citri (ATCC 1015), Candida albicans (ATCC 10231), Fusarium oxysporum (ATCC 16608) and Ustilago maydis (ATCC 14826). The best actinomycetes based on size of inhibition and broad spectrum of activity coded PAN 25, PAN 41, PAN 75 and PAN 110 were selected for further bioassay. Extraction of antibiotics was carried out using ethyl acetate. The selected actinomycetes were characterized using molecular technique that involved extraction of 16S rRNA gene, PCR and phylogenetic studies. The data obtained was analyzed using Statistical Package of Social Sciences (SPSS) version 25 software. The number of the isolated actinomycetes varied significantly (F=3.315 P=0.04218). There was no significant difference in the zones of inhibition between the isolates PAN 25, PAN 41, PAN 75 and PAN 110 (F=0.8928 P=0.5431). In addition, there was no significant difference in the zones of inhibition between the extracted antibiotics (F=1.726424 P=0.126281). The 16S rRNA gene sequence and nucleotide BLAST from NCBI revealed that isolate PAN 25 had similarity with Streptomyces variabilis, isolate PAN 41 (S. indiaensis), isolate PAN 75 (S. luteogriseus strain ZG728) and isolate PAN 110 (S. acrimycini strain K30). Menengai crater had actinomycetes that inhibited growth of the selected actinomycetes. There is need of isolating actinomycetes from other unique regions in Kenya. Large scale production of antibiotics from the selected actinomycetes need also to be carried out.

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Pharmacological Activities of Streptomyces Species PO-178 Isolated from Rhizosphere Soil of Agumbe, Karnataka, India

Cited by 8 publications

References 30 publications

Screening for Antimicrobial Agents Producing Actinomycetes Isolated from Agricultural Soils in Hilla/Iraq

Screening for Antimicrobial Agents Producing Actinomycetes Isolated from Agricultural Soils in Hilla/Iraq

Antibacterial Activity of Bioactive Streptomyces Species Mppo-02 Against Clinical Isolates of Burn, Dental Caries and Urinary Tract Infections

Molecular Characterization of Four Antibiotic Producing Actinomycetes Isolated from Menengai Crater

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