Cytological characterization of Jatropha curcas callus in different periods of cultivation

Santos, Dalilhia Nazaré dos; Nunes, Claudinéia Ferreira; Soares, Joyce Dória Rodrigues; Valente, Thaís Cainã Teixeira; Alves, Eduardo; Labory, Cláudia Regina Gontijo; Pasqual, Moacir

doi:10.1590/s1984-70332013000400002

Cited by 4 publications

(5 citation statements)

References 22 publications

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“…In the present study, starch grains could be observed until the 28 th day of culture, after which cell masses lose their embryogenic characteristics ( Figure 4F and H). Embryogenic structures do not continue their development during the stationary phase because cells go into decline and lose their intracellular organization due to the process of senescence (Santos et al, 2013). Therefore, transfer to a new culture medium can maintain the continuity of development, resulting in a greater proliferation of cells with embryogenic potential.…”

Section: Discussionmentioning

confidence: 99%

Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg

Rossato

Schumacher

Netto

et al. 2019

Acta Sci. Biol. Sci.

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Campomanesia adamantium is a native plant species of Brazilian Cerrado with diverse economic potential and great medicinal importance. Its sexual propagation is impaired by the recalcitrance of its seeds, which prevents effective and profitable propagation. With the purpose of establishing commercial crops and minimizing the extractive use of vegetal resources, the aim of the present study was to induce embryogenic calli in nodal segments of gabirobeira, and to determine and characterize their embryogenic phase through the establishment of a growth curve based on cellular characteristics. Calli were induced using nodal segments inoculated in WPM culture medium without the addition of hormones (control) and with different concentrations of 2,4-D, IAA, IBA, NAA or picloram. Cytochemical and SEM analyses revealed cellular characteristics of the formation of meristematic centers that indicated 4.14 μM of picloram to be the best treatment for induction of embryogenic calli, and demonstrating their embryogenic potential. The treatment was used to establish a callus growth curve, from which it was inferred that calli should be transferred to new culture media on the 28th day to maintain cell viability

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Section: Discussionmentioning

confidence: 99%

Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg

Rossato

Schumacher

Netto

et al. 2019

Acta Sci. Biol. Sci.

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“…In the linear phase, few cells were capable of multiplication, and the observed decrease in the mitotic index is indicative that the cells are prepared for cell differentiation (Santos et al 2013). However, in this period, cell growth and development are more evident (Landa et al 2000), because the cells grow in volume (Feitosa et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…These pigments may be related to senescence of callus, due to stress caused by the possible shortage of nutrients, water and/or growth regulators, resulting in an increase of free radicals, toxic to the plant (Valle 2003). The low cell viability and mitotic index are because cells are not able to divide, become senescent and die (Santos et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Cell Viability, Mitotic Index and Callus Morphology of Byrsonima verbascifolia (Malpighiaceae)

Chiavegatto

Castro

Marçal

et al. 2015

Tropical Plant Biol.

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The combined analysis of cell viability, mitotic index and callus morphology of species of commercial interest may favor the identification of cell masses with embryogenic characteristics at early stages of cultivation, allowing the development or improvement of protocols for in vitro plant regeneration through indirect embryogenesis. Byrsonima verbascifolia L. (DC.) (Malpighiaceae) is a Brazilian medicinal species native to the Cerrado, which has a low seed germination rate due to tegument dormancy. This study aimed to identify cells with embryogenic characteristics during growth of callus of B. verbascifolia, by assessing cell viability, mitotic index and cell morphology. Callus was induced from leaf explants and inoculated on MS medium with 4.52 μ M 2,4-D and 4.44 μ M benzylaminopurine (BAP). Friable calli were obtained at four distinct growth phases: lag (until the 30th), exponential (from 31st to the 70th day), linear (from 71st to the 100th day) and decline (from 101st to the 120th day). Calli with 20 and 30 days showed cells with embryogenic characteristics, that is, isodiametric shape, juxtaposed, with small vacuoles, thin cell wall and presence of amyloplasts.After 120 days, calli were mostly elongated cells with lower cell viability and mitotic index.

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“…Calos advindos de um dos cotilédones, cultivados em meio MS, suplementado com 0,5 mg L -1 de 2,4-D + 2,0 mg L -1 de picloram + 0,5 mg L -1 de cinetina + 2,0 mg L -1 de BAP, foram repicados duas vezes, em intervalos de 30 dias. Após a segunda repicagem, foi estabelecida a curva de crescimento dos calos por meio da determinação da massa fresca a partir do dia da inoculação (tempo 0), em intervalos de sete dias, durante 112 dias (Santos et al, 2013).…”

Section: Metodologiaunclassified

Curva de crescimento de calos de Enterolobium contortisiliquum induzidos in vitro

Rodrigues

Cavalcanti

Soares

et al. 2022

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O objetivo do trabalho foi determinar in vitro a curva de crescimento de calos de Enterolobium contortisiliquum. Foram utilizados calos advindos dos cotilédones de E. contortisiliquum cultivados em meio MS, suplementado com 0,5 mg L-1 de 2,4-D + 2,0 mg L-1 de picloram + 0,5 mg L-1 de cinetina + 2,0 mg L-1 de BAP e repicados duas vezes, em intervalos de 30 dias. Após a segunda repicagem, foi estabelecida a curva de crescimento dos calos por meio da determinação da massa fresca (mg) a partir do dia da inoculação (tempo 0). O delineamento experimental foi inteiramente casualizado com 17 tratamentos, constituídos pelos intervalos de avaliação (0, 7, 14, 21, 28, 35, 42, 49, 56, 63, 70, 77, 84, 91, 98, 105 e 112 dias), e 24 repetições. O E. contortisiliquum apresentou cinco fases de desenvolvimento de calos: exponencial, linear, desaceleração, estacionário e declínio. A curva de crescimento do calo para a espécie E. contortisiliquum começou com a fase exponencial e permaneceu nesta fase dos dias 0 a 7. Já o período de crescimento linear em que diminuiu a taxa de divisão celular e aumentou a área celular foi observado entre os dias 7 e 14. Enquanto a fase de desaceleração ocorreu dos 14 aos 28 dias. Conclui-se que a curva de crescimento dos calos de E. contortisiliquum é uma sigmoide com cinco fases e ao final da desaceleração é o momento de se realizar a repicagem dos calos, em média aos 28 dias de cultivo in vitro.

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Cytological characterization of Jatropha curcas callus in different periods of cultivation

Abstract: -The aimed was characterization and determining the time for

Cited by 4 publications

References 22 publications

Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg

Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg

Cell Viability, Mitotic Index and Callus Morphology of Byrsonima verbascifolia (Malpighiaceae)

Curva de crescimento de calos de Enterolobium contortisiliquum induzidos in vitro

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