Embryogenic potential of the callus of gabirobeira, Campomanesia adamantium (Cambess) O. Berg

Abstract

Campomanesia adamantium is a native plant species of Brazilian Cerrado with diverse economic potential and great medicinal importance. Its sexual propagation is impaired by the recalcitrance of its seeds, which prevents effective and profitable propagation. With the purpose of establishing commercial crops and minimizing the extractive use of vegetal resources, the aim of the present study was to induce embryogenic calli in nodal segments of gabirobeira, and to determine and characterize their embryogenic phase through the establishment of a growth curve based on cellular characteristics. Calli were induced using nodal segments inoculated in WPM culture medium without the addition of hormones (control) and with different concentrations of 2,4-D, IAA, IBA, NAA or picloram. Cytochemical and SEM analyses revealed cellular characteristics of the formation of meristematic centers that indicated 4.14 μM of picloram to be the best treatment for induction of embryogenic calli, and demonstrating their embryogenic potential. The treatment was used to establish a callus growth curve, from which it was inferred that calli should be transferred to new culture media on the 28th day to maintain cell viability

Indirect organogenesis for high frequency shoot regeneration of two cultivars of Sansevieria trifasciata Prain differing in fiber production

“…5 c,e) (Table S1 , S2 ). This high efficiency of callogenesis in snake plant is similar to that observed in Campomanesia adamantium 57 . In monocots, callogenesis performance has been quantified for only a few plant species so far.…”

Cytokinin induces the development of gabirobeira root cuttings

“…however, it presents high genetic variability by sexual propagation and has recalcitrant seeds that do not tolerate storage, which makes the uniform reproduction of progenies difficult (EMER et al, 2018a). An alternative is the employ of vegetative propagation techniques, it includes air layering (TELEGINSKI et al, 2018), cuttings (MARTINS et al, 2015EMER et al, 2016;EMER et al, 2018b) or in vitro (ROSSATO et al, 2015;GOELZER et al, 2019;ROSSATO et al, 2019;MACHADO et al, 2020), always associated with plant growth regulators.…”

Cultivo >i<in vitro>/i< de cerejeira-do-Rio-Grande (>i<Eugenia involucrata>/i< DC.) e de cambucizeiro (>i<Campomanesia phaea>/i< (O. Berg) Landrum)

“…Em espécies da família Myrtaceae nativas do Brasil, estudos de cultivo in vitro vêm sendo conduzidos com o objetivo de entender o comportamento de cada espécie quando dispostas aos mais diversos componentes do meio de cultura e aprimorar o uso da técnica. São encontrados na literatura relatos promissores do uso da cultura de tecidos para essas espécies, como, por exemplo, a indução de embriogênese somática em camucamuzeiro (Myrciaria dubia (Kunth) McVaugh) (ARAÚJO et al, 2015); jaboticabeira (Plinia peruviana (Poir) Govaerts) (SILVEIRA et al, 2020); pitangueira (Eugenia uniflora L.) (STEFENON et al, 2020); e goiabeira serrana (STEFANELLO et al, 2005;CANGAHUALA-INOCENTE et al, 2007;FRAGA et al, 2012); de micropropagação de explantes provenientes de plantas adultas em pitangueira (Eugenia uniflora L.) (FREIRE et al, 2018); camucamuzeiro (Myrciaria dubia (Kunth) McVaugh) (ARAUJO et al, 2016); e araçazeiro (Psidium cattleyanum) ) e de multiplicação por explantes provenientes de plântulas germinadas in vitro em guabijuzeiro (Myrcianthes pungens O. Berg) D. Legrand (SOUZA et al, 2011;SOUZA et al, 2020); pitangueira (Eugenia uniflora L.) (SILVA et al, 2014); araçazeiro do campo (Psidium schenckianum Kiaersk) e araçazeiro verdadeiro (Psidium guineense Swartz) (SANTOS et al, 2021); casaqueira (Campomanesia rufa (O. Berg) Nied) ; gabirobeira (Campomanesia adamantium (Cambess) O. Berg) (ROSSATO et al, 2019); guabirobeira (Campomanesia xanthocarpa O. Berg) (MACHADO et al, 2020); e cambucizeiro (Myrciaria tenella O. Berg.)…”