Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari)

Abstract

<jats:p>Mycoplasma suis is a hemotropic bacteria of red blood cells and the causative agent of swine eperythrozoonosis. Diagnosis of infection may be reached by direct examination of blood smears; however, the use of polymerase chain reaction (PCR) of the 16S RNA gene of M. suis improves the sensitivity and specificity of detection. The aim of this study was to screen peccaries (Tayassu tajacu and T. pecari) for M. suis infection using a specific conventional PCR. A total of 28 blood samples from captive collared and white-lipped peccaries were collected, DNA extracted and a specific M. suis PCR assay performed. All samples were negatives by both blood smear examination and PCR testing. To verify the presence of amplifiable DNA, PCR for beta-actin gene was performed in all samples. This study was part of an active surveillance program, which is crucial for monitoring animal health status, particularly in wildlife species.</jats:p>

Hemotropic mycoplasmas in little brown bats (Myotis lucifugus)

“…appear to have co-evolved with many animal species. The development of molecular assays, primarily targeting the 16S rRNA gene of these microbes, has resulted in the recent recognition of several novel animal and human hemoplasmas [15,17-19,44-47]. This study represents the first report of a novel, and as yet incompletely characterized hemotropic Mycoplasma species in little brown bats, with an overall prevalence of 47%.…”

A quantitative TaqMan PCR assay for the detection of Mycoplasma suis

“…Blood DNA samples from 22 captive collared peccaries ( Tayassu tajacu ) and six white‐lipped peccaries ( Tayassu pecari ) that were previously negative by a cPCR for Myc. suis (Vieira et al. 2011) were also analysed.…”

PCR and qPCR for detection of Porcine Circovirus type 2 (PCV2) in captive white-lipped (Tayassu pecari) and collared (Tayassu tajacu) peccaries from Southern Brazil

“…A standard curve constructed with plasmid (pGEM Promega) was also included with four dilutions points from 2.96x10 5 to 2.96x10 2 . Although peccaries may be potential reservoirs of infectious diseases, captive animals from the present study were also previously negative for Mycoplasma suis by PCR (VIEIRA et al, 2011).…”

“…Accordingly, the aim of this study was to identify the PCV2 in whole blood samples by conventional and quantitative PCR (qPCR) of previously surveyed approved under IBAMA and ethical committee for other pathogens (VIEIRA et al, 2011) of adults captive peccaries from Curitiba Zoo in Paraná State, Brazil (16 collared and 6 white-lipped).…”

“…EDTA-blood samples previously surveyed for hemotropic mycoplasmas (VIEIRA et al, 2011) were used in this study. DNA was extracted using an available commercial kit (Ilustra TM GFX TM Genomic Blood DNA Purification Kit, GE Healthcare, Buckinghamshire, UK), according to the manufacturers´ instructions.…”