A quantitative TaqMan PCR assay for the detection of Mycoplasma suis

Guimarães, Ana M. S.; Vieira, Rafael Felipe da Costa; Poletto, Rosangela; Vemulapalli, Ramesh; Santos, Andrea Pires dos; Moraes, Wanderlei de; Cubas, Zalmir Silvino; Santos, Leonilda Correia dos; Marchant‐Forde, Jeremy N.; Timenetsky, Jorge; Biondo, Alexander Welker; Messick, Joanne B.

doi:10.1111/j.1365-2672.2011.05053.x

Cited by 31 publications

(54 citation statements)

References 26 publications

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“…Molecular techniques, mainly qPCR, have been shown to be accurate and quick tests for the diagnosis of M. suis, which enable its detection and quantification (GUIMARÃES et al, 2011). Despite the apparent importance of M. suis to the swine industry worldwide, reports of this hemoplasma species in swine herds around the world have been rare.…”

Section: Introductionmentioning

confidence: 99%

High occurrence of Mycoplasma suis infection in swine herds from non-technified farms in Mossoró, state of Rio Grande do Norte, Northeastern Brazil

Toledo

Leite

Gonçalves

et al. 2016

Rev. Bras. Parasitol. Vet.

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Mycoplasma suis, the etiological agent of swine hemoplasmosis, has been neglected in swine herds around the world. Swine hemoplasmosis is frequently associated with hemolytic anemia, disgalacty, infertility and immunosuppression, and it results in significant economic losses. This study investigates the occurrence of M. suis in non-technified swine herds in the northeastern region of Brazil using quantitative PCR (qPCR) based on the 16S rRNA gene. Between March and August 2013, blood samples from 147 swine were collected during slaughter in the city of Mossoró, state of Rio Grande do Norte, northeastern Brazil. One hundred and twelve samples (76.19%) were positive for M. suis by qPCR assays. The range of Cqs and quantification (copies of a M. suis-16S rRNA gene fragment/µL) was 20.86–37.89 and 1.64×101–6.64×107, respectively. One can conclude that M. suis infection have high occurrence (76,19%) in non-technified swine-rearing systems in Mossoró in the state of Rio Grande do Norte, Brazil.

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Section: Introductionmentioning

confidence: 99%

High occurrence of Mycoplasma suis infection in swine herds from non-technified farms in Mossoró, state of Rio Grande do Norte, Northeastern Brazil

Toledo

Leite

Gonçalves

et al. 2016

Rev. Bras. Parasitol. Vet.

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show abstract

“…The detection of carrier animals is correlated with sensitivity of the method (1,4,8,12,15,17). In the present study, it was possible to detect a massive presence of M. suis in blood by examination of stained blood smears.…”

Section: Discussionmentioning

confidence: 66%

“…In the present study, it was possible to detect a massive presence of M. suis in blood by examination of stained blood smears. On the basis of worldwide knowledge and due to the use of sensitive PCR methods (4,15), the widespread prevalence of asymptomatic carriers were confirmed. This provides an explanation why two blood samples derived from control animals (not experimentally infected) from the standard farm were the ones with low positive results available for this method.…”

Section: Discussionmentioning

confidence: 99%

“…The primers and Taqman probe for M. suis were based on the sequence of 16S rRNA gene (3), previously described by Guimares et al (4).…”

Section: Methodsmentioning

confidence: 99%

“…Diagnosis of infection is based on clinical symptoms and the observation of microorganisms on the surface of red blood cells in blood smears. Another recently developed method involves the detection of microorganisms by the use of molecular methods (4,10,12,14). Blood smear methods, however, are too imprecise when compared to the molecular methods used for detecting infections.…”

Section: Introductionmentioning

confidence: 99%

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Application of quantitative PCR for detection of Mycoplasma suis in blood samples

Jabłoński

Borowska

Zębek

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was to develop and validate a real-time PCR method, using a TaqMan probe, for quantification of Mycoplasma suis in porcine blood. No PCR signals with closely related non-haemotrophic mycoplasmas were obtained. The detection limit of PCR for plasmid combined with blood DNA was determined to be 10 3 /reaction (5 µL of DNA) (1.2x10 5 target copies in 1 mL of blood). The linearity of real-time PCR (near 1) indicates its use as a quantitative method. Real-time and quantitative PCR were sensitive and specific for the detection and quantification of M. suis in the blood of animals with acute and chronic form of eperythrozoonosis. Developed quantitative PCR cannot be used to detect carrier animals with a small amount of M. suis in their blood. The validity of real-time PCR used in the studies was confirmed by the low inter-and intra-assay coefficients of variation. This fact confirms the applicability of the assay in other laboratories.

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Establishment of molecular diagnostics targeting the 23S ribosomal RNA gene for the detection of Mycoplasma suis infection in Thai domestic pigs

Thongmeesee

Sri-in²,

Kaewthamasorn³

et al. 2023

Acta Tropica

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A quantitative TaqMan PCR assay for the detection of Mycoplasma suis

Abstract: TaqMan qPCR is an accurate and quick test for detection of Myc. suis infected pigs, which can be used on varied instrumentation platforms.

Cited by 31 publications

References 26 publications

High occurrence of Mycoplasma suis infection in swine herds from non-technified farms in Mossoró, state of Rio Grande do Norte, Northeastern Brazil

High occurrence of Mycoplasma suis infection in swine herds from non-technified farms in Mossoró, state of Rio Grande do Norte, Northeastern Brazil

Application of quantitative PCR for detection of Mycoplasma suis in blood samples

Establishment of molecular diagnostics targeting the 23S ribosomal RNA gene for the detection of Mycoplasma suis infection in Thai domestic pigs

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