Genetic and phenotypic diversity of Magnaporthe oryzae from leaves and panicles of rice in commercial fields in the State of Goiás, Brazil

Silva, Gisele Barata da; Prabhu, A. S.; Filippi, Marta Cristina Corsi de; Trindade, M. da G.; Araújo, Leila Garcês de; Zambolim, Laércio

doi:10.1590/s1982-56762009000200002

Cited by 13 publications

(12 citation statements)

References 23 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results showed a high level of genetic diversity, and blast strains could be clustered based on geographic distribution and collection time. Silva et al [18] used rep-PCR analysis with two primer sequences from Pot2 to assess genetic diversity of rice blast strains from Brazil. Xu et al [19] used RAPD, REMAP, rep-PCR markers, and AVR gene sequences to examine the genetic diversity of rice blast fungus strains from China.…”

Section: Genetic Diversity Of Pyricularia Oryzae Using Srap and Issr mentioning

confidence: 99%

Morphological Characterization and Genetic Diversity of Rice Blast Fungus, Pyricularia oryzae, from Thailand Using ISSR and SRAP Markers

Longya

Talumphai

Jantasuriyarat

2020

JoF

View full text Add to dashboard Cite

Rice blast disease is caused by the ascomycete fungus Pyricularia oryzae and is one of the most destructive rice diseases in the world. The objectives of this study were investigating various fungal morphological characteristics and performing a phylogenetic analysis. Inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers were used to examine the genetic variation of 59 rice blast fungus strains, including 57 strains collected from different fields in Thailand and two reference strains, 70-15 and Guy11. All isolates used in this study were determined to be P. oryzae by internal transcribed spacer (ITS) sequence confirmation. A total of 14 ISSR primers and 17 pairs of SRAP primers, which produced clear and polymorphic bands, were selected for assessing genetic diversity. A total of 123 polymorphic bands were generated. The similarity index value for the strains ranged from 0.25 to 0.95. The results showed that the blast fungus population in Thailand has both morphological and genetic variations. A high level of genetic variation, or genome adaptation, is one of the fungal mechanisms that could overcome host resistance to avoid host recognition. Results from this research study could bring substantial benefits and ultimately help to understand the blast fungal pathogen genome and the population structure in Thai blast fungus.regions with primers targeting open reading frames (ORFs) [5]. The molecular markers differ in cost, speed, complication, labor, degree of polymorphism, and repeatability. ISSR and SRAP marker techniques are fast and low cost, they do not require sequence information, have high repeatability, and use only one-step PCR. They provide highly discriminating information with good reproducibility and are relatively abundant, while AFLP and RAPD are more labor intensive and time consuming [4,6]. We characterized blast fungal morphology, performed DNA sequence analyses of the internal transcribed spacer (ITS), and used 20 ISSR and 30 SRAP markers to assess the genetic diversity of 59 rice blast strains, including 57 strains collected from Thai rice-growing areas and two reference strains, 70-15 and Guy11. The information obtained from this study will help to understand the population structure and evolution of rice blast fungus in Thailand. Materials and Methods Rice Blast MaterialsA total of 59 rice blast strains were used, including 57 strains collected from Thai rice-growing areas representing central, northern, and north-eastern parts of Thailand. Two rice blast strains, 70-15 and Guy11, were used as reference strains (Table 1). The stock of each blast strain as in filter paper was cultured and DNA was extracted using the cetyl-trimethyl-ammonium-bromide (CTAB) method described by Longya et al. [7].

show abstract

Section: Genetic Diversity Of Pyricularia Oryzae Using Srap and Issr mentioning

confidence: 99%

Morphological Characterization and Genetic Diversity of Rice Blast Fungus, Pyricularia oryzae, from Thailand Using ISSR and SRAP Markers

Longya

Talumphai

Jantasuriyarat

2020

JoF

View full text Add to dashboard Cite

show abstract

“…The first results indicated that each locus had a range of different alleles from 2 to 10 and the results of SSR showed genes which were polymorphic in nature [Liu et al 2008]. Silva et al [2009] evaluated the genetic diversity of P. grisea on rice in Brazil. The genetic analysis of isolates showed the highest genetic diversity population belonged to 103 haplotypes in Bonanca and 49 in Primavera.…”

Section: Introductionmentioning

confidence: 99%

GENETIC DIVERSITY OF RICE BLAST FUNGUS POPULATIONS (Pyricularia grisea) USING MOLECULAR MARKERS

El-Wahsh

El–Refaee

Emeran³

et al. 2016

Journal of Agricultural Chemistry and Biotechnology

View full text Add to dashboard Cite

Pyricularia grisea fungus which causing rice blast disease is one of the main pathological races threats to rice crop in Egypt and worldwide. Thirty isolates out of known 144 strains of this fungus were selected according to geographical locations. Isolates of P. grisea, were analyzed by SSR markers to determine the amount of genetic variability among these races. Sixteen primers out of 26 were amplified and produced a total of 83 alleles . Number of alleles per marker ranged from 3 for C7-1.4, P9-0.4 and P9-1.9 to 9 for H6-0.8-5 primer, with an average of 5.2. The polymorphic information content (PIC) values were high for all markers with an average of 0.70 and ranged from as low as 0.51 for C7-1. 4 to high of 0.83 for H6-0.8-5 marker. UPGMAcluster-analysis based on genetic distance coefficients clearly separated all isolates of P. grisea. The results showed that the studied isolates were classified into 4 lineages by cutting off the dendrogram at 0.70 similar linkage level. Group D was the major group and represented most of those isolates. The average similarities within cluster was greater than the average similarities between clusters. In general, the results of this investigation confirmed that microsatellite markers would be useful and suitable for determining the genetic diversity in P. grisea populations.

show abstract

“…This fungus is considered as highly variable and high degree of pathogenic variation (pathotypes) is observed in the field [8,9]. Sexual reproduction can enhance and influence the genotypic variability of M. oryzae populations where the recombinant progenies may have new capability to infect different host cultivars.…”

Section: Introductionmentioning

confidence: 99%

Mating Types Analysis of Magnaporthe oryzae Populations by Molecular Methods

Samanta¹,

Dhua²,

Nayak³

et al. 2014

TOBIOTJ

View full text Add to dashboard Cite

Rice blast is a devastating disease which is caused by the heterothallic fungus Magnaporthe oryzae. Compatible sexual recombination which occurs between two M. oryzae strains of different mating types, can enhance genetic variability. Assessment of mating type alleles is used as a marker to measure population diversity. Forty six isolates of M. oryzae were collected from infected rice leaves from various ecosystems of coastal Odisha, India, and the mating type analysis using molecular markers was carried out. MAT1-1 mating type was dominating in all the ecosystems and MAT1-2 was found to be present in uplands as well as in irrigated fields. Both mating types could be found in the same field in irrigated ecosystem. The disease spread was very fast vertically as well as horizontally in those fields resulting in blast lesions looking as 'green islands (gi) produced in senescence leaves', and MAT1-2 was found to be associated with all gi lesions. Consequently, the management of the disease in those plots was very difficult. Interestingly, ribosomal RNA IGS region could not be amplified in MAT1-2 isolates but consistent amplification was obtained in MAT1-1 mating type isolates.

show abstract

Genetic and phenotypic diversity of Magnaporthe oryzae from leaves and panicles of rice in commercial fields in the State of Goiás, Brazil

Cited by 13 publications

References 23 publications

Morphological Characterization and Genetic Diversity of Rice Blast Fungus, Pyricularia oryzae, from Thailand Using ISSR and SRAP Markers

Morphological Characterization and Genetic Diversity of Rice Blast Fungus, Pyricularia oryzae, from Thailand Using ISSR and SRAP Markers

GENETIC DIVERSITY OF RICE BLAST FUNGUS POPULATIONS (Pyricularia grisea) USING MOLECULAR MARKERS

Mating Types Analysis of Magnaporthe oryzae Populations by Molecular Methods

Contact Info

Product

Resources

About