Mating Types Analysis of Magnaporthe oryzae Populations by Molecular Methods

Samanta, Soma; Dhua, Urmila; Nayak, Shubhransu; Behera, Lopamudra; Mukherjee, Arup

doi:10.2174/1874070701408010006

Cited by 8 publications

(4 citation statements)

References 22 publications

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“…Also, MAT1‐1 and MAT1‐2 were observed in rain‐fed as well as irrigated fields. The invasive manner of the fungus can be explained through the fact that in the irrigated ecosystem, both mating types are present inside one field which is in agreement with our findings (Samanta et al., 2014).…”

Section: Discussionsupporting

confidence: 92%

Sexual recombination and genetic diversity in Iranian populations of Pyrenophora teres

Dokhanchi

Arzanlou

Abed-Ashtiani

2021

Journal of Phytopathology

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Pyrenophora teres is an economically important pathogen of barley worldwide causing net blotch diseases. In this study, the frequency and mating-type alleles's distribution as well as genetic diversity of P. teres were assessed on micro-spatial scale and also on macro-geographical scale to find out whether a sexual cycle is possible within its Iranian populations. A multiplex PCR assay was developed for simultaneous identification and recognition of P. teres f. teres and P. teres f. maculata and to screen their mating-type alleles. 118 isolates of P. teres were procured from barley fields of East Azerbaijan province during 2017-2018. Among 89 isolates of P. teres f. teres examined, 39 isolates were found as MAT1-1 and 50 isolates were found to be MAT1-2.Among 29 isolates of P. teres f. maculata examined, 17 isolates were found as MAT1-1 and 12 isolates were found to be MAT1-2 with the dominancy belonging to MAT1-1.Nearly same mating-type alleles distribution within and between various populations of P. teres f. teres (1:1 ratio; χ 2 = 1.36) and P. teres f. maculata (1:1 ratio; χ 2 = 0.862) was observed. Genetic diversity assessment of 118 P. teres isolates was carried out through inter-simple sequence repeat (ISSR) analysis and results revealed that there was 70.83% and 63.89% polymorphism among the isolates of P. teres f. teres and P. teres f. maculata, respectively. No relation was observed between the isolates and their mating as well as geographical type. Among collected isolates (89 P. teres f. teres and 29 P. teres f. maculata isolates), the gene diversity was generally similar (h = 0.280 and h = 0.238 for P. teres f. teres and P. teres f. maculata, respectively). Based on our findings and referring to the prior data on the structure of P. teres population, we conclude that in the majority of tested areas, this fungal species might have the potential to undergo regular sexual recombination cycles.

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Section: Discussionsupporting

confidence: 92%

Sexual recombination and genetic diversity in Iranian populations of Pyrenophora teres

Dokhanchi

Arzanlou

Abed-Ashtiani

2021

Journal of Phytopathology

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“…Nearly all rice blast isolates in the field are males and thus are unable to cross with one another, however many isolates from other grasses are hermaphrodites (Bonman, 1992), therefore it is a possibility that the isolates from rice and from other grasses can cross and produce fertile strains. These fertile strains can enhance the genotypic variability of M. oryzae populations where the progenies may have new capabilities to infect different rice cultivars (Samanta et al, 2014).…”

Section: Biology Of Rice Blast Pathogenmentioning

confidence: 99%

The pathogen and control management of rice blast disease

Zakaria¹,

Misman²

2018

MJM

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Outbreak of rice blast disease is a serious problem in all rice growing regions worldwide and is a threat to rice production. In Malaysia, the outbreak of rice blast is a recurrent problem where rice plant remain vulnerable to the disease. Nowadays, the generic name of rice blast pathogen is Pyricularia oryzae and Magnaporthe oryzae is the synonym. Rice pathogen infects mainly aerial parts of rice plant causing rotting and blast on the leaves, stems, peduncles, panicles and seeds leading to crop failure. Rice blast pathogen population is regarded as highly variable and consisted of multiple pathotypes which contribute to genetic diversity as well virulence diversity of the pathogen. The pathotypes can only be determined through pathogenicity test using a set of differential rice cultivars which generally differ by one or more resistance genes. Knowledge on the rice blast virulence and host resistant is important to manage the disease. Among the control methods, utilizing resistant cultivars is considered as the most effective approach to manage rice blast disease. Therefore, development of blast resistant cultivar becomes a priority.

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“…The presence of MAT1 ‐ 2 allele in the population at a higher rate (>90%) was also reported by Tansian and Parinthawong (2018) in Thai isolates. Samanta et al (2014) reported the occurrence of MAT1 ‐ 2 population in the uplands and irrigated ecosystems of Central India, and they also reported the occurrence of both mating types in the irrigated ecosystem. A higher MAT1‐2 mating type distribution was also reported from North‐East and Eastern India (Imam et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Multilocus sequence analysis and identification of mating-type idiomorphs distribution in Magnaporthe oryzae population of Karnataka state of India

Chittaragi

Pramesh

Naik

et al. 2022

Journal of Applied Microbiology

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Aims:To investigate the genetic diversity, population structure and mating-type distribution among the eco-distinct isolates of Magnaporthe oryzae from Karnataka, India. Methods and Results:A set of 38 isolates of M. oryzae associated with leaf blast disease of rice were collected from different rice ecosystems of Karnataka, India, and analysed for their diversity at actin, β-tubulin, calmodulin, translation elongation factor 1α (TEF-1α), and internal transcribed spacer (ITS) genes/region. The isolates were grouped into two clusters based on the multilocus sequence diversity, the majority being in cluster-IA (n = 37), and only one isolate formed cluster-IB. Population structure was analysed using 123 SNP data to understand the genetic relationship.Based on K = 2 and ancestry threshold of >70%, blast strains were classified into two subgroups (SG1 and SG2) whereas, based on K = 4 and ancestry threshold of >70%, blast strains were classified into four subgroups (SG1, SG2, SG3 and SG4).We have identified 13 haplotype groups where haplotype group 2 was predominant (n = 20) in the population. The Tajima's and Fu's Fs neutrality tests exhibited many rare alleles. Further, the mating-type analysis was also performed using MAT1 genespecific primers to find the potentiality of sexual reproduction in different ecosystems. The majority of the isolates (54.5%) had MAT1-2 idiomorph, whereas 45.5% of the isolates possessed MAT1-1 idiomorph.Conclusions: The present study found the genetically homogenous population of M. oryzae by multilocus sequence analysis. Both mating types, MAT1-1 and MAT1-2, were found within the M. oryzae population of Karnataka. Significance and impact of study:The study on the population structure and sexual mating behaviour of M. oryzae is important in developing region-specific blastresistant rice cultivars. This is the first report of MAT1 idiomorphs distribution in the M. oryzae population in any Southern state of India.

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Mating Types Analysis of Magnaporthe oryzae Populations by Molecular Methods

Cited by 8 publications

References 22 publications

Sexual recombination and genetic diversity in Iranian populations of Pyrenophora teres

Sexual recombination and genetic diversity in Iranian populations of Pyrenophora teres

The pathogen and control management of rice blast disease

Multilocus sequence analysis and identification of mating-type idiomorphs distribution in Magnaporthe oryzae population of Karnataka state of India

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