GENETIC DIVERSITY OF RICE BLAST FUNGUS POPULATIONS (Pyricularia grisea) USING MOLECULAR MARKERS

El-Wahsh, S.; El–Refaee, Y. Z.; Emeran, Amero A.; Mashaal, S.F.; Arafa, Ramadan A.

doi:10.21608/jacb.2016.40781

Cited by 4 publications

(5 citation statements)

References 13 publications

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“…As shown in Table 9 , the H e values for all SSR markers used in this study varied from 0.94 to 1.00, with an average of 0.98. The findings were in agreement with the observation of [ 44 , 45 , 46 ]. The highest H e value (1.00) was recorded for RM23 and 518.…”

Section: Resultssupporting

confidence: 93%

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Genetic Diversity of Selected Rice Genotypes under Water Stress Conditions

Gaballah

Metwally

Skalický

et al. 2020

Plants

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Drought is the most challenging abiotic stress for rice production in the world. Thus, developing new rice genotype tolerance to water scarcity is one of the best strategies to achieve and maximize high yield potential with water savings. The study aims to characterize 16 rice genotypes for grain and agronomic parameters under normal and drought stress conditions, and genetic differentiation, by determining specific DNA markers related to drought tolerance using Simple Sequence Repeats (SSR) markers and grouping cultivars, establishing their genetic relationship for different traits. The experiment was conducted under irrigated (normal) and water stress conditions. Mean squares due to genotype × environment interactions were highly significant for major traits. For the number of panicles/plants, the genotypes Giza179, IET1444, Hybrid1, and Hybrid2 showed the maximum mean values. The required sterility percentage values were produced by genotypes IET1444, Giza178, Hybrid2, and Giza179, while, Sakha101, Giza179, Hybrid1, and Hybrid2 achieved the highest values of grain yield/plant. The genotypes Giza178, Giza179, Hybrid1, and Hybrid2, produced maximum values for water use efficiency. The effective number of alleles per locus ranged from 1.20 alleles to 3.0 alleles with an average of 1.28 alleles, and the He values for all SSR markers used varied from 0.94 to 1.00 with an average of 0.98. The polymorphic information content (PIC) values for the SSR were varied from 0.83 to 0.99, with an average of 0.95 along with a highly significant correlation between PIC values and the number of amplified alleles detected per locus. The highest similarity coefficient between Giza181 and Giza182 (Indica type) was observed and are susceptible to drought stress. High similarity percentage between the genotypes (japonica type; Sakha104 with Sakha102 and Sakha106 (0.45), Sakha101 with Sakha102 and Sakha106 (0.40), Sakha105 with Hybrid1 (0.40), Hybrid1 with Giza178 (0.40) and GZ1368-S-5-4 with Giza181 (0.40)) was also observed, which are also susceptible to drought stress. All genotypes are grouped into two major clusters in the dendrogram at 66% similarity based on Jaccard’s similarity index. The first cluster (A) was divided into two minor groups A1 and A2, in which A1 had two groups A1-1 and A1-2, containing drought-tolerant genotypes like IET1444, GZ1386-S-5-4 and Hybrid1. On the other hand, the A1-2 cluster divided into A1-2-1 containing Hybrid2 genotype and A1-2-2 containing Giza179 and Giza178 at coefficient 0.91, showing moderate tolerance to drought stress. The genotypes GZ1368-S-5-4, IET1444, Giza 178, and Giza179, could be included as appropriate materials for developing a drought-tolerant variety breeding program. Genetic diversity to grow new rice cultivars that combine drought tolerance with high grain yields is essential to maintaining food security.

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Section: Resultssupporting

confidence: 93%

“…This marker could be useful in MAS for these characters in rice. The results were similar to those obtained by [ 46 , 54 ].…”

Section: Resultssupporting

confidence: 92%

Genetic Diversity of Selected Rice Genotypes under Water Stress Conditions

Gaballah

Metwally

Skalický

et al. 2020

Plants

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“…DNA markers could be used to differentiate genes involved in rice drought tolerance (Kumar et al 2016;Barik et al 2019;Prakash et al 2019;Upadhyaya and Panda 2019;Panda et al 2021). The PIC value provided information regarding determining marker values to detect polymorphisms (El-Wahsh et al 2016). There was one marker that was classified as low (RM 248) and two markers classified as moderate (RM 72 and RM 228), while the other 17 markers were classified as highly informative.…”

Section: Discussionmentioning

confidence: 99%

Selection of drought-tolerant rice genotypes under cajuput (Melaleuca cajuputi subsp. cajuputi) agroforestry system

TARYONO,

SUPRIYANTA,

WULANDARI

et al. 2023

Biodiversitas

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Abstract. Taryono, Supriyanta, Wulandari RA, Nurmansyah, Ambarwati E, Arsana IGKD, Aristya VE, Purba AE, Aisya AW, Alam T. 2023. Selection of drought-tolerant rice genotypes under cajuput (Melaleuca cajuputi subsp. cajuputi) agroforestry system. Biodiversitas 24: 4791-4802. Rice production can be increased by expanding rice cultivation area on agroforestry land. However, planting rice in agroforestry system, which is generally rainfed, might cause drought stress and eventually can reduce rice yields. The study aimed to select rice genotypes under drought stress conditions in a rainfed agroforestry system with cajuput or kayu putih (Melaleuca cajuputi subsp. cajuputi Powell). Ten promising rice lines and four control cultivars were grown during the dry season from March-July 2022 at the Menggoran Forest Management Resort, Playen Forest Section, Yogyakarta Forest Management, Indonesia. The observation was conducted on drought stress tolerance (sensitivity and recovery), molecular identification, and yield of rice cultivars. The results showed that G5 was the genotype with drought tolerance and had the best recovery compared to the other promising rice lines and control cultivars. The molecular identification of the 14 rice genotypes using 20 SSR markers detected 108 alleles with a PIC value of 0.707. The G5, G4, and G9 had higher yields than the control cultivars. The BLUE showed that the yield of G5, G4, and G9 were 6.59, 5.93 and 5.50 tons ha-1, respectively, while for BLUP by 6.45, 5.87 and 5.43 tons ha-1, respectively. Furthermore, there were four clusters consisting of cluster 1 (G9), cluster 2 (G7), cluster 3 (G5 and Inpago 12), and cluster 4 (Inpari 42, G6, Inpari 30 Ciherang Sub 1, Situ Bagendit, G3, G4, G10, G2, G1, and G8). The findings of this study recommend G4, G5, and G9 as promising rice lines which tolerant to drought stress under M. cajuputi agroforestry system and can be utilized in future plant breeding programs.

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“…PIC value defines the value of marker for detecting polymorphism within a population under investigation, depending number of detectable alleles and their frequency distribution, providing an estimate of discriminating power of the marker [43]. As it shown in Table 2, the PIC values for the SSR used in this study varied from 0.83 to 0.99 with an average of 0.94.…”

Section: Polymorphic Information Content Value (Pic)mentioning

confidence: 98%

Identification of genetic diversity among some promising lines of rice under drought stress using SSR markers

Gaballah

Fiaz

Wang

et al. 2021

Journal of Taibah University for Science

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Fourteen rice lines and one cultivar were investigated for morphological traits and genetic diversity under normal and drought stress condition. Mean squares of years, environment and lines × environments were significant for all traits under observation, except for days to heading and grain yield/plant in years, days to heading in environment and days to heading and harvest index in lines × environments, respectively. The 10 SSR markers, covering seven chromosomes (1, 2, 4, 5, 6, 8 and 9) generated polymorphic alleles with sum of 72 alleles on seven chromosomes with 9 markers across the fifteen rice lines. Furthermore, genetic diversity values for all SSR markers varied from 0.94 to 1.00 with an average of 0.98. In addition, the PIC values ranged from 0.83 to 0.99 with an average value of 0.94. The promising lines of present investigation can be utilized in future plant breeding programs.

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GENETIC DIVERSITY OF RICE BLAST FUNGUS POPULATIONS (Pyricularia grisea) USING MOLECULAR MARKERS

Cited by 4 publications

References 13 publications

Genetic Diversity of Selected Rice Genotypes under Water Stress Conditions

Genetic Diversity of Selected Rice Genotypes under Water Stress Conditions

Selection of drought-tolerant rice genotypes under cajuput (Melaleuca cajuputi subsp. cajuputi) agroforestry system

Identification of genetic diversity among some promising lines of rice under drought stress using SSR markers

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