Effect of neem seed extracts on the development of the Soybean Cysts Nematode

Silva, Júlio C.T.; Oliveira, R. D. L.; Jham, Gulab N.; Aguiar, Naylor D.C.

doi:10.1590/s1982-56762008000300001

Cited by 10 publications

(12 citation statements)

References 14 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results obtained by SILVA et al (2008) show that occured a reduction of 90% in the number of eggs and 84% in the number of cysts caused in soybean by the nematode H. glycines (ICHINOHE, 1952), when used the aqueous extract and methanolic, obtained from neem plants.…”

Section: Resultsmentioning

confidence: 99%

Nematicidal activityin vitroandin vivoof neem oil onMeloidogyne incognita

Dourado¹,

Lima²,

Muraishi³

2013

Revista Brasileira De Tecnologia Aplicada Nas Ciências Agrárias

View full text Add to dashboard Cite

Considering the losses caused by nematodes in the different crops, chemical substances with nematicidal effect have been investigated and found in various plants. The neem oil is presented as an alternative to manage this pathogen. The aim of this study was to evaluate in vitro, the action of different doses of neem oil on the hatching and immobility of Meloidogyne incognita juveniles and in vivo in order to verify the data about the efficacy of neem oil to control nematodes of galls in tomato plants. In vitro assays were installed in laboratory conditions and in a completely randomized design with four replications. The experiment consisted of the following treatments: 1. Distilled water (100%); 2. Furadan (5%); 3. Neem oil (1%); 4. Neem oil (5%); 5. Neem oil (10%). For the experiment done in greenhouse, the experimental design was completely randomized with five replicates. The experiment consisted of the following treatments: 1. Control; 2. Neem oil in the soil; 3. Neem oil foliar; 4. Neem oil in the soil + leaf. The use of neem oil in vitro was effective only in the immobility of M. incognita at concentrations of 1%. The neem oil reduces the number of galls of M. incognita when in foliar application + soil at concentrations of 1%.Key words: Azadirachta indica A. Juss, immobility, nematode galls, hatching rate. Atividade nematicida in vitro e in vivo do óleo de nim sobre Meloidogyne incógnita ResumoConsiderando as perdas causadas por nematoides às diversas culturas, substâncias químicas com efeito nematicida têm sido pesquisadas e encontradas em várias plantas. O óleo de nim se apresenta como uma alternativa para manejar esse patógeno. O objetivo desta pesquisa foi avaliar in vitro a ação de diferentes doses do óleo de nim sobre a eclosão e imobilidade de juvenis de Meloidogyne incognita e in vivo com a finalidade de comprovar os dados relativos à eficácia do óleo de nim no controle de nematoides de galhas em plantas de tomate. Os ensaios in vitro foram instalados em condições de laboratório e em delineamento inteiramente casualizado com quatro repetições. O experimento constou dos seguintes tratamentos: 1. Água destilada (100%); 2. Furadan (5%); 3. Óleo de nim (1%); 4. Óleo de nim (5%); 5. Óleo de nim (10%). Para o experimento realizado em casa de vegetação, o delineamento experimental utilizado foi o inteiramente casualizado, com cinco repetições. O experimento constou dos seguintes tratamentos: 1. Testemunha; 2. Óleo de nim via solo; 3. Óleo de nim via foliar; 4. Óleo de nim via solo + foliar. A utilização do óleo de nim in vitro foi efetivo apenas na imobilidade de M. incognita nas concentrações de 1%. O óleo de nim reduz o número de galhas de M. incognita quando aplicado via foliar + solo nas concentrações de 1%. Palavras-chave:Azadirachta indica A. Juss, imobilidade, nematoides das galhas, taxa de eclosão. Actividad nematicida in vitro e in vivo de Aceite de Neem sobre Meloidogyne incognita ResumenTeniendo en cuenta las pérdidas causadas por nematodos en diferentes cultivos, distintas sustancias...

show abstract

Section: Resultsmentioning

confidence: 99%

Nematicidal activityin vitroandin vivoof neem oil onMeloidogyne incognita

Dourado¹,

Lima²,

Muraishi³

2013

Revista Brasileira De Tecnologia Aplicada Nas Ciências Agrárias

View full text Add to dashboard Cite

show abstract

“…A similar trend was observed by Neves et al (2009), in which the ketone extracts and the garlic chloroform, mustard and pepper, showed no significant reduction in the population of nematodes and the number of galls of M. incognita in tomato. According to Silva et al (2008), the hexane extract of neem seeds was not effective in increasing Heterodera glycines I. J2 mortality, when compared to aqueous and methanolic extracts, and this was explained by the hexane extract's composition which is essentially lipids. The Cinnamomum cassia P. chloroform extract also showed no nematicide effect against the pine nematode Bursaphelenchus xylophilus K. (NGUYEN et al, 2009).…”

Section: Experiments 1: Effect Of Crambe Extractsmentioning

confidence: 99%

“…Even the balanced use of chemicals can have long-term effects, leading to selection of resistant plant pathogens and pollution, resulting in adverse consequences for the environment and society. However, many studies have been conducted in the search for new active ingredients of natural origin, such as plant extracts, which have secondary metabolites with antimicrobial activity or inducing resistance (NEVES et al, 2009;STANGARLIN et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Several plants show a resistance against nematodes and this is attributed to the presence of certain active compounds, such as flavonoids and rosmarinic acid (CABONI et al, 2013), allyl isothiocyanate, acryloyl isothiocyanate, bezyl isothiocyanate (NEVES et al, 2009;WU et al, 2011;AISSANI et al, 2013), azadirachtin, desacetylnimbim, desacetylsalannin, nimbin, and salannin (SILVA et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…These plant extracts have shown potential as raw material for the production of natural nematicides, as described by Franzener et al (2005), Elbadri et al (2008), andSilva et al (2008). The nematicidal activity can depend on both the plant used for the preparation of the extract and the solvent used in the extraction (AMARAL et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nematicidal activity of crambe extracts on Meloidogyne spp.

Coltro-Roncato

Stangarlin

Gonçalves

et al. 2016

SCA

View full text Add to dashboard Cite

Alternative methods for the control of nematodes, such as the use of plant secondary metabolites, can be explored for integrated pest management systems. The objective of this work was to assess the best solvent for obtaining allyl isothiocyanate from Crambe abyssinica leaves, and the effects of this extract on Meloidogyne incognita and M. javanica. Dry leaves of C. abyssinica at 200 mg L -1 were used to prepare extracts by using water (by infusion and grinding), acetone, water + ethanol (hydroalcoholic extraction), methanol, hexane, and chloroform as solvents. Following the evaporation of the solvents, the residue was resuspended in water for use in the experiments. Distilled water and chemical nematicide were used as control treatments. Once the most effective extracts were defined, the following dosages of dried crambe leaves were used: 0, 200, 300, 400, and 500 mg L -1 . High performance liquid chromatography (HPLC) was used to quantify the allyl isothiocyanate present in the extracts. After the solvents evaporated, the residues were eluted with water and used in assays with 200 eggs for the hatching test or 200 second stage juveniles (J2) for mobility and mortality tests. The hydroalcoholic extract was the most effective in reducing the hatching of M. incognita and M. javanica juveniles, by 71.6 and 74.4 percentage points, respectively. The mortality of M. incognita and M. javanica in the hydroalcoholic extract was 93.2 and 64.4%, respectively, followed by the methanol extract (17.6 and 34%) and the extract obtained by grinding (9.2 and 28%). The hydroalcoholic extract at 250 mg L -1 showed high nematicidal effect. The HPLC analysis of the extracts revealed that only the methanol and hydroalcoholic extracts had allyl isothiocyanate, indicating that the inhibitory effects on the hatching, mobility, and mortality were not solely attributed to the presence of this compound.

show abstract